NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 6999R-3

Symbol CG6999 Full Name CG6999

CG No CG6999 Old CG No CG6999

Synonyms CG6999

Accession No (Link to NCBI) NM_132295.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGAAAGTGGAGGAGACTAAGC

Primer Seq. 3'

aatctagaggtaccCTAGAGCGATTTTTTTGGCCC

Predicted Fragment Size

IR fragment full Seq

0001 TGAAAGTGGA GGAGACTAAG CTAGACGAGG CGCGCTGGGG TAAGCCGCAG ACAAAGGAAG 
0061 GTGAGTCTGC AAATGGGATA GCAAATCCCT CAAATGACGA TAAAAAGGAG CTGGCCAATT 
0121 TCAAAGCCAC CTTCAATTCC TGGGCCCCCG AGAAGAAACG CGAGAAGATG CACAAGGTAG 
0181 GCGTCATCTT AATATCCAAC ATACCCAAGG ACATGGACGG GGACTGCCTG AAGGAAATCA 
0241 TGAACTTGCA CAGCGTCGTC GGCAGAGTTT ACGTGCAGTC GGAAACGCTG TCAAGTTTCA 
0301 AGACAAAGAA GAACATGCGT AAGGGCTGGG TGGAGTTCAT TTCGAAAAGT GGGGCCAAAA 
0361 AAATCGCTCT AG

in silico PCR Fragment

0001 TGAAAGTGGA GGAGACTAAG CTAGACGAGG CGCGCTGGGG TAAGCCGCAG ACAAAGGAAG 
0061 GTGAGTCTGC AAATGGGATA GCAAATCCCT CAAATGACGA TAAAAAGGAG CTGGCCAATT 
0121 TCAAAGCCAC CTTCAATTCC TGGGCCCCCG AGAAGAAACG CGAGAAGATG CACAAGGTAG 
0181 GCGTCATCTT AATATCCAAC ATACCCAAGG ACATGGACGG GGACTGCCTG AAGGAAATCA 
0241 TGAACTTGCA CAGCGTCGTC GGCAGAGTTT ACGTGCAGCC GGAAACGCTG TCAAGTTTCA 
0301 AGACAAAGAA GAACATGCGT AAGGGCTGGG TGGAGTTCAT TTCGAAAAGT GGGGCCAAAA 
0361 AAATCGCTCT AG

Assemble Data


6999R-3.IR_full       1   TGAAAGTGGAGGAGACTAAGCTAGACGAGGCGCGCTGGGGTAAGCCGCAGACAAAGGAAG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6999R-3.in silico     1   TGAAAGTGGAGGAGACTAAGCTAGACGAGGCGCGCTGGGGTAAGCCGCAGACAAAGGAAG 60


6999R-3.IR_full       61  GTGAGTCTGCAAATGGGATAGCAAATCCCTCAAATGACGATAAAAAGGAGCTGGCCAATT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6999R-3.in silico     61  GTGAGTCTGCAAATGGGATAGCAAATCCCTCAAATGACGATAAAAAGGAGCTGGCCAATT 120


6999R-3.IR_full       121 TCAAAGCCACCTTCAATTCCTGGGCCCCCGAGAAGAAACGCGAGAAGATGCACAAGGTAG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6999R-3.in silico     121 TCAAAGCCACCTTCAATTCCTGGGCCCCCGAGAAGAAACGCGAGAAGATGCACAAGGTAG 180


6999R-3.IR_full       181 GCGTCATCTTAATATCCAACATACCCAAGGACATGGACGGGGACTGCCTGAAGGAAATCA 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6999R-3.in silico     181 GCGTCATCTTAATATCCAACATACCCAAGGACATGGACGGGGACTGCCTGAAGGAAATCA 240


6999R-3.IR_full       241 TGAACTTGCACAGCGTCGTCGGCAGAGTTTACGTGCAGTCGGAAACGCTGTCAAGTTTCA 300
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
6999R-3.in silico     241 TGAACTTGCACAGCGTCGTCGGCAGAGTTTACGTGCAGCCGGAAACGCTGTCAAGTTTCA 300


6999R-3.IR_full       301 AGACAAAGAAGAACATGCGTAAGGGCTGGGTGGAGTTCATTTCGAAAAGTGGGGCCAAAA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6999R-3.in silico     301 AGACAAAGAAGAACATGCGTAAGGGCTGGGTGGAGTTCATTTCGAAAAGTGGGGCCAAAA 360


6999R-3.IR_full       361 AAATCGCTCTAG 372
                          ||||||||||||
6999R-3.in silico     361 AAATCGCTCTAG 372

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	354	0	0	0	NM_132295.1	CG6999-RA (CG6999), mRNA
85.87	304	29	4	10	NM_167175.1	CG32706-RA (CG32706), mRNA
0	0	4	48	73	NM_167174.1	CG32708-RA (CG32708), mRNA
0	0	3	3	2	NM_141826.2	CG10898-RA (CG10898), mRNA
0	0	3	2	4	NM_138189.2	CG6905-RA (CG6905), mRNA
0	0	2	9	3	NM_169926.1	CG17838-RD, transcript variant D (CG17838), mRNA
0	0	2	9	2	NM_169927.1	CG17838-RA, transcript variant A (CG17838), mRNA
0	0	2	9	2	NM_169928.1	CG17838-RC, transcript variant C (CG17838), mRNA
0	0	2	9	2	NM_142656.2	CG17838-RB, transcript variant B (CG17838), mRNA
0	0	2	9	2	NM_169929.1	CG17838-RF, transcript variant F (CG17838), mRNA
0	0	2	9	2	NM_169925.1	CG17838-RE, transcript variant E (CG17838), mRNA
0	0	2	6	5	NM_165522.1	CG11112-RB, transcript variant B (CG11112), mRNA
0	0	2	6	5	NM_136427.1	CG11112-RA, transcript variant A (CG11112), mRNA
0	0	2	2	3	NM_140524.2	CG12301-RA (CG12301), mRNA
0	0	1	3	3	NM_132107.2	CG3224-RA (CG3224), mRNA
0	0	1	2	4	NM_001031957.1	CG33796-RA (CG33796), mRNA
0	0	0	16	44	NM_132695.1	CG10993-RA (CG10993), mRNA
0	0	0	5	9	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	0	5	7	NM_057617.3	CG10497-RA, transcript variant A (Sdc), mRNA
0	0	0	5	5	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	0	4	7	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	0	4	7	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	0	4	7	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	0	4	6	NM_168992.2	CG10520-RA (tub), mRNA
0	0	0	4	5	NM_141278.2	CG2663-RB, transcript variant B (CG2663), mRNA
0	0	0	4	4	NM_164683.1	CG31641-RB, transcript variant B (stai), mRNA
0	0	0	4	4	NM_164682.1	CG31641-RC, transcript variant C (stai), mRNA
0	0	0	4	3	NM_135977.1	CG15133-RA (CG15133), mRNA
0	0	0	4	3	NM_078867.2	CG5526-RA (Dhc36C), mRNA
0	0	0	4	3	NM_169053.2	CG2663-RA, transcript variant A (CG2663), mRNA

Wing Disc

GFP

Puc

Caspase

DAPI

Merge

Figure

JNK signal

Caspase-3 signal

Dcompartment		Penetrance

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