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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 6289R-3 
 Symbol CG6289  Full Name CG6289 
 CG No CG6289  Old CG No CG6289 
 Synonyms CG6289 
 Accession No (Link to NCBI) NM_140950.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTCGGATTTCTGGGCCTTTTTG 
 Primer Seq. 3' 
aatctagaggtaccGATAATTATACTCGCCCCCTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TCGGATTTCT GGGCCTTTTT GGCATGGTGC TCAGTTTGCG GGCCCATATA TGTGCAAATA 
0061 CTGTTGATAC GAAGAGTCTA TTACAAAAAA ATGACGGACG CAAGGCTTCA ATTTGCCCTC 
0121 AACCTGCTGC AAATGGAATC CACGCATTTA AACCTGGAAA ACTTTGCGAT GACGCCATTT 
0181 TCCACGTGGT CACTTATGAT CATGTTCTAC GAGGGAGCAG AGGGGACTAC CTTGAAGCAG 
0241 ATACGTGATG TCCTGGCGAT CTATGTCGAT TATCCAACAC TGCGCAGGTG GTACGAGGAT 
0301 GTCCGAGCTT ATCACTATTT AAATTCGGAA AACACAAAAC TGTTTTCGCT GCGTTACGCT 
0361 TACTACGACG ACGTAGGAGA TTTAGAACTC GTTAAAGGGT ACAACAGTGT AGTCCTAGAA 
0421 GGAGTAGGAG AAGGAAATGT GGTTCTTCGA GAGGGTCGGC CAAGGGGTGT GGACTTCGAC 
0481 CAGGGGGCGA GTATAATTAT C 
 in silico PCR Fragment 
0001 TCGGATTTCT GGGCCTTTTT GGCATGGTGC TCAGTTTGCG GGCCCATATA TGTGCAAATA 
0061 CTGTTGATAC GAAGAGTCTA TTAC-AAAAA ATGACGGACG CAAGGCTTCA ATTTGCCCTC 
0121 AACCTGCTGC AAATGGAATC CACGCATTTA AACCTGGAAA ACTTTGCGAT GACGCCATTT 
0181 TCCACGTGGT CACTTATGAT CATGTTCTAC GAGGGAGCAG AGGGGACTAC CTTGAAGCAG 
0241 ATACGTGATG TCCTGGCGAT CTATGTCGAT TATCCAACAC TGCGCAGGTG GTACGAGGAT 
0301 GTCCGAGCTT ATCACTATTT AAATTCGGAA AACACAAAAC TGTTTTCGCT GCGTTACGCT 
0361 TACTACGACG ATGTAGGAGA TTTAGAACTC GTGAAAGGGT ACAACAGTGT AGTCCTAGAA 
0421 GGAGTAGGAG AAGGAAATGT GGTTCTTCGA GAGGGTCGGC CAAGGGGTGT GGACTTCGAC 
0481 CAGGGGGCGA GTATAATTAT C 
 Assemble Data 

6289R-3.IR_full       1   TCGGATTTCTGGGCCTTTTTGGCATGGTGCTCAGTTTGCGGGCCCATATATGTGCAAATA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     1   TCGGATTTCTGGGCCTTTTTGGCATGGTGCTCAGTTTGCGGGCCCATATATGTGCAAATA 60


6289R-3.IR_full       61  CTGTTGATACGAAGAGTCTATTACAAAAAAATGACGGACGCAAGGCTTCAATTTGCCCTC 120
                          |||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||
6289R-3.in silico     61  CTGTTGATACGAAGAGTCTATTAC-AAAAAATGACGGACGCAAGGCTTCAATTTGCCCTC 120


6289R-3.IR_full       121 AACCTGCTGCAAATGGAATCCACGCATTTAAACCTGGAAAACTTTGCGATGACGCCATTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     121 AACCTGCTGCAAATGGAATCCACGCATTTAAACCTGGAAAACTTTGCGATGACGCCATTT 180


6289R-3.IR_full       181 TCCACGTGGTCACTTATGATCATGTTCTACGAGGGAGCAGAGGGGACTACCTTGAAGCAG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     181 TCCACGTGGTCACTTATGATCATGTTCTACGAGGGAGCAGAGGGGACTACCTTGAAGCAG 240


6289R-3.IR_full       241 ATACGTGATGTCCTGGCGATCTATGTCGATTATCCAACACTGCGCAGGTGGTACGAGGAT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     241 ATACGTGATGTCCTGGCGATCTATGTCGATTATCCAACACTGCGCAGGTGGTACGAGGAT 300


6289R-3.IR_full       301 GTCCGAGCTTATCACTATTTAAATTCGGAAAACACAAAACTGTTTTCGCTGCGTTACGCT 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     301 GTCCGAGCTTATCACTATTTAAATTCGGAAAACACAAAACTGTTTTCGCTGCGTTACGCT 360


6289R-3.IR_full       361 TACTACGACGACGTAGGAGATTTAGAACTCGTTAAAGGGTACAACAGTGTAGTCCTAGAA 420
                          ||||||||||| |||||||||||||||||||| |||||||||||||||||||||||||||
6289R-3.in silico     361 TACTACGACGATGTAGGAGATTTAGAACTCGTGAAAGGGTACAACAGTGTAGTCCTAGAA 420


6289R-3.IR_full       421 GGAGTAGGAGAAGGAAATGTGGTTCTTCGAGAGGGTCGGCCAAGGGGTGTGGACTTCGAC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
6289R-3.in silico     421 GGAGTAGGAGAAGGAAATGTGGTTCTTCGAGAGGGTCGGCCAAGGGGTGTGGACTTCGAC 480


6289R-3.IR_full       481 CAGGGGGCGAGTATAATTATC 501
                          |||||||||||||||||||||
6289R-3.in silico     481 CAGGGGGCGAGTATAATTATC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140950.1  CG6289-RA (CG6289), mRNA 
43.98   212  56  6  10  NM_140949.1  CG6663-RA (CG6663), mRNA 
0   0  4  3  3  NM_079395.2  CG9695-RA (Dab), mRNA 
0   0  2  2  4  NM_170542.1  CG18729-RA (zwilch), mRNA 
0   0  2  2  2  NM_144467.1  CG3267-RA (CG3267), mRNA 
0   0  1  6  8  NM_134718.4  CG31795-RA, transcript variant A (ia2), mRNA 
0   0  1  5  6  NM_143314.2  CG5634-RA (dsd), mRNA 
0   0  1  5  5  NM_144352.2  CG32025-RA (desert), mRNA 
0   0  1  4  11  NM_132903.1  CG9906-RA (CG9906), mRNA 
0   0  1  4  5  NM_079382.5  CG4609-RA, transcript variant A (fax), mRNA 
0   0  1  4  5  NM_001014587.2  CG4609-RC, transcript variant C (fax), mRNA 
0   0  1  4  5  NM_176336.3  CG4609-RB, transcript variant B (fax), mRNA 
0   0  1  4  5  NM_001014586.2  CG4609-RD, transcript variant D (fax), mRNA 
0   0  1  3  11  NM_134516.2  CG32529-RA, transcript variant A (CG32529), mRNA 
0   0  1  3  7  NM_080045.2  CG6093-RA (abo), mRNA 
0   0  1  3  3  NM_078670.2  CG7092-RA (Dhc16F), mRNA 
0   0  1  3  2  NM_136617.1  CG8788-RA (CG8788), mRNA 
0   0  1  2  6  NM_164966.2  CG6464-RA (salm), mRNA 
0   0  1  2  2  NM_144130.1  CG18336-RA (CG18336), mRNA 
0   0  1  2  2  NM_057693.3  CG4799-RA (Pen), mRNA 
0   0  0  7  7  NM_001014682.1  CG33553-RB, transcript variant B (Doa), mRNA 
0   0  0  7  7  NM_001043300.1  CG33553-RF, transcript variant F (Doa), mRNA 
0   0  0  7  7  NM_001014680.1  CG33553-RA, transcript variant A (Doa), mRNA 
0   0  0  7  7  NM_001043299.1  CG33553-RG, transcript variant G (Doa), mRNA 
0   0  0  5  3  NM_132570.1  CG15927-RA (CG15927), mRNA 
0   0  0  4  7  NM_167226.1  CG32686-RB, transcript variant B (CG32686), mRNA 
0   0  0  4  7  NM_167227.1  CG32686-RA, transcript variant A (CG32686), mRNA 
0   0  0  4  5  NM_135007.1  CG3251-RA (CG3251), mRNA 
0   0  0  4  5  NM_164401.3  CG31795-RB, transcript variant B (ia2), mRNA 
0   0  0  4  5  NM_001043218.1  CG33097-RB, transcript variant B (CG33097), mRNA 

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