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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5999R-2 
 Symbol CG5999  Full Name CG5999 
 CG No CG5999  Old CG No CG5999 
 Synonyms CG5999 
 Accession No (Link to NCBI) NM_141972.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACCGTGCTGAAGCCAGTGGTT 
 Primer Seq. 3' 
aatctagaggtaccTTACGCCGCTCGGAAAGATGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACCGTGCTGA AGCCAGTGGT TAACCATAAA AACATAACCG TGATCCAAGT GCCGCTTAGT 
0061 AAGGAAGAAG CCCAGCAAAT GAGCGATACG ATTGGAGCGA TGTCCAAGAA CGACAATAGC 
0121 AACATGGCCT TATCCCTGCT CCGGATGTCG GATCAAATGG ATTTCATGAT CAGAAAGAAT 
0181 GCTGAGACCC TGATGGACGA TCGAGTGAGG GATCTGTACC TGAACCGTGG AAACAAGTTC 
0241 GACCTGGTTA TTTCTGGATA CTTTATGAAC GATTTCCAGC TCGGATTCGC AAGGAAAGTC 
0301 AACGCACCCG TCATCGTATT GGCCACAATG CCTCCCAATC AACTATTGAA CCCCCTGATC 
0361 GGTAATCCCC TGGAGGTTTC CTATGCCGGA ATCAGTAATC CTGCAGAGGG CAGTAAAGCC 
0421 GTAACCTTCC AACGGCGTTT ATCCAGCCTA TATGCAAAGC CTCGGTTTCG GTGTCTTTTC 
0481 TCATCTTTCC GAGCGGCGTA A 
 in silico PCR Fragment 
0001 ACCGTGCTGA AGCCAGTGGT TAACCATAAA AACATAACCG TGATCCAAGT GCCGCTTAGT 
0061 AAGGAAGAAG CCCAGCAAAT GAGCGATACG ATTGGAGCGA TGTCCAAGAA CGACAATAGC 
0121 AACATGGCCT TATCCCTGCT CCGGATGTCG GATCAAATGG ATTTCATGAT CAGAAAGAAT 
0181 GCCGAGACCC TGATGAACGA TCGAGTGAGG GATCTGTACC TGAACCGTGG AAACAAGTTC 
0241 GACCTGGTTA TTTCTGGATA CTTTATGAAC GATTTCCAGC TCGGATTCGC AAGGAAAGTC 
0301 AACGCACCCG TCATCGTATT GGCCACAATG CCTCCCAATC ATCTATTGAA CCCCCTGATC 
0361 GGTAATCCCC TGGAGGTTTC CTATGCCGGA ATCAGTAATC CTGCAGAGGG CAGTAAAGCC 
0421 GTAACCTTCC AACGGCGTTT ATCCAG-CTA TATGCAAAGC CTCGGTTTCG GTGTCTTTTC 
0481 TCATCTTTCC GAGCGGCGTA A 
 Assemble Data 

5999R-2.IR_full       1   ACCGTGCTGAAGCCAGTGGTTAACCATAAAAACATAACCGTGATCCAAGTGCCGCTTAGT 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     1   ACCGTGCTGAAGCCAGTGGTTAACCATAAAAACATAACCGTGATCCAAGTGCCGCTTAGT 60


5999R-2.IR_full       61  AAGGAAGAAGCCCAGCAAATGAGCGATACGATTGGAGCGATGTCCAAGAACGACAATAGC 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     61  AAGGAAGAAGCCCAGCAAATGAGCGATACGATTGGAGCGATGTCCAAGAACGACAATAGC 120


5999R-2.IR_full       121 AACATGGCCTTATCCCTGCTCCGGATGTCGGATCAAATGGATTTCATGATCAGAAAGAAT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     121 AACATGGCCTTATCCCTGCTCCGGATGTCGGATCAAATGGATTTCATGATCAGAAAGAAT 180


5999R-2.IR_full       181 GCTGAGACCCTGATGGACGATCGAGTGAGGGATCTGTACCTGAACCGTGGAAACAAGTTC 240
                          || |||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     181 GCCGAGACCCTGATGAACGATCGAGTGAGGGATCTGTACCTGAACCGTGGAAACAAGTTC 240


5999R-2.IR_full       241 GACCTGGTTATTTCTGGATACTTTATGAACGATTTCCAGCTCGGATTCGCAAGGAAAGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     241 GACCTGGTTATTTCTGGATACTTTATGAACGATTTCCAGCTCGGATTCGCAAGGAAAGTC 300


5999R-2.IR_full       301 AACGCACCCGTCATCGTATTGGCCACAATGCCTCCCAATCAACTATTGAACCCCCTGATC 360
                          ||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||
5999R-2.in silico     301 AACGCACCCGTCATCGTATTGGCCACAATGCCTCCCAATCATCTATTGAACCCCCTGATC 360


5999R-2.IR_full       361 GGTAATCCCCTGGAGGTTTCCTATGCCGGAATCAGTAATCCTGCAGAGGGCAGTAAAGCC 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5999R-2.in silico     361 GGTAATCCCCTGGAGGTTTCCTATGCCGGAATCAGTAATCCTGCAGAGGGCAGTAAAGCC 420


5999R-2.IR_full       421 GTAACCTTCCAACGGCGTTTATCCAGCCTATATGCAAAGCCTCGGTTTCGGTGTCTTTTC 480
                          |||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
5999R-2.in silico     421 GTAACCTTCCAACGGCGTTTATCCAG-CTATATGCAAAGCCTCGGTTTCGGTGTCTTTTC 480


5999R-2.IR_full       481 TCATCTTTCCGAGCGGCGTAA 501
                          |||||||||||||||||||||
5999R-2.in silico     481 TCATCTTTCCGAGCGGCGTAA 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141972.1  CG5999-RA (CG5999), mRNA 
0.2   1  11  52  79  NM_141971.1  CG5724-RA (CG5724), mRNA 
0.2   1  3  3  2  NM_164900.1  CG5680-RB (bsk), mRNA 
0   0  1  6  2  NM_168898.1  CG32436-RA (CG32436), mRNA 
0   0  1  4  7  NM_001042796.1  CG6775-RC, transcript variant C (rg), mRNA 
0   0  1  4  7  NM_080023.1  CG6775-RA, transcript variant A (rg), mRNA 
0   0  1  4  7  NM_167028.1  CG6775-RB, transcript variant B (rg), mRNA 
0   0  1  3  2  NM_138059.1  CG13575-RA (CG13575), mRNA 
0   0  1  2  4  NM_142056.2  CG9312-RA (CG9312), mRNA 
0   0  1  2  4  NM_139501.2  CG2114-RA (FR), mRNA 
0   0  1  2  2  NM_136711.2  CG11866-RA (CG11866), mRNA 
0   0  0  7  6  NM_176056.2  CG15257-RB, transcript variant B (Tim17b2), mRNA 
0   0  0  6  8  NM_164906.1  CG31873-RA (CG31873), mRNA 
0   0  0  5  4  NM_175984.1  CG13388-RD, transcript variant D (Akap200), mRNA 
0   0  0  5  4  NM_058112.3  CG13388-RA, transcript variant A (Akap200), mRNA 
0   0  0  5  2  NM_132817.1  CG9215-RA (CG9215), mRNA 
0   0  0  4  7  NM_001043133.1  CG32045-RD, transcript variant D (fry), mRNA 
0   0  0  4  7  NM_168357.2  CG32045-RB, transcript variant B (fry), mRNA 
0   0  0  4  6  NM_165887.1  CG30047-RA (CG30047), mRNA 
0   0  0  4  4  NM_136845.1  CG9003-RA (CG9003), mRNA 
0   0  0  4  4  NM_136907.1  CG13169-RA (CG13169), mRNA 
0   0  0  4  3  NM_135553.2  CG5355-RA (CG5355), mRNA 
0   0  0  3  9  NM_206174.2  CG8201-RE, transcript variant E (par-1), mRNA 
0   0  0  3  9  NM_206176.2  CG8201-RD, transcript variant D (par-1), mRNA 
0   0  0  3  9  NM_206175.2  CG8201-RF, transcript variant F (par-1), mRNA 
0   0  0  3  7  NM_078647.3  CG9907-RA, transcript variant A (para), mRNA 
0   0  0  3  7  NM_001042815.1  CG9907-RB, transcript variant B (para), mRNA 
0   0  0  3  7  NM_001042816.1  CG9907-RC, transcript variant C (para), mRNA 
0   0  0  3  5  NM_001032067.1  CG6713-RH, transcript variant H (Nos), mRNA 
0   0  0  3  5  NM_169594.2  CG3389-RA (Cad88C), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
++ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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