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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 5770R-1 
 Symbol CG5770  Full Name CG5770 
 CG No CG5770  Old CG No CG5770 
 Synonyms BcDNA:LP06072, CG5770 
 Accession No (Link to NCBI) NM_137438.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGAATATTCTGTGCACCGTGC 
 Primer Seq. 3' 
aatctagaggtaccGTTGTTCTGCGTTGTGTTGGT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TGAATATTCT GTGCACCGTG CTTATCTTCG CCAGCGTTTT GGCGGCCAAC GCCAAGCCCA 
0061 ATATACCAGA TCCAAAGTGC CTTGGATCAA TATATTGGTG CATGCTCTGC AATCTGGATA 
0121 CATTTGTTTC CGAGGATATA GACCACGCAG TGCTTCAACA TATACATAGC CCATGCTGAA 
0181 TCAAGTGGCT CGCCACTTTC TCTGAGTTCC TATACAGGAC TGCATACACC ACCGCCAATG 
0241 CCCAGATCGC CAATTTGACC GCCCAGGCGA GAGCAGCAGC AGAAGACCTA TCAGTCCGAC 
0301 GTTTCAACAC TCTGCAGTGC CTTTTACCGC CTGCGACAGC AACAATGATA CCGCCAACTT 
0361 CTTCAACTGC TACGCGAATG CAGCTGATAA TGACGTCTCT GTGATCTACA CCCTGGCCAG 
0421 CAACGCTGCC AGCTCGGCCA CCTCCTTCAG CTCGGGCATC CAAGCCATCC AGGACACCGA 
0481 GTACCAGTGC ACCAACACAA CGCAGAACAA C 
 in silico PCR Fragment 
0001 TGAATATTCT GTGCACCGTG CTTATCTTCG CCAGCGTTTT GGCGGCCAAC GCCAAGCCCA 
0061 ATA-ACCAGA TCCAGAGTGC CTTGGATCAG TAT-TTGGTG CATGCTC-GC AGTCTGGATA 
0121 CATTTGTTTC CGAGGATAT- GACCACGCAG TGCTTCAACT TATACAT-GC CTATGCTGAA 
0181 TCAAGTGGCT -GCCACTTTC TC-GAGTTCC TAT-CAGGAC TGCAT-CACC ACCGCCAATG 
0241 CCCAGATCGC CAATTTGACC GCCCAGGCG- GAGCAGCAGC AGAAGACCTA TCAGTCCGAC 
0301 GTTTCAACAC TCTGCAGTGC C-TTTACCGC CTGCGACAGC AACAATGATA CCGCAAACTT 
0361 CTTCAACTGC TACGCGAATG CTGCTGATAA TGACGTCTCT GTGATCTACA ACCTGGCCAG 
0421 CAACGCTGCC AGCTCGGCCA CCTCCTTCAG CTCGGGCATC CAAGCCATCC AGGACACCGA 
0481 GTACCAGTGC ACCAACACAA CGCAGAACAA C 
 Assemble Data 

5770R-1.IR_full       1   TGAATATTCTGTGCACCGTGCTTATCTTCGCCAGCGTTTTGGCGGCCAACGCCAAGCCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5770R-1.in silico     1   TGAATATTCTGTGCACCGTGCTTATCTTCGCCAGCGTTTTGGCGGCCAACGCCAAGCCCA 60


5770R-1.IR_full       61  ATATACCAGATCCAAAGTGCCTTGGATCAATATATTGGTGCATGCTCTGCAATCTGGATA 120
                          ||| |||||||||| |||||||||||||| ||| ||||||||||||| ||| ||||||||
5770R-1.in silico     61  ATA-ACCAGATCCAGAGTGCCTTGGATCAGTAT-TTGGTGCATGCTC-GCAGTCTGGATA 120


5770R-1.IR_full       121 CATTTGTTTCCGAGGATATAGACCACGCAGTGCTTCAACATATACATAGCCCATGCTGAA 180
                          ||||||||||||||||||| ||||||||||||||||||| ||||||| ||| ||||||||
5770R-1.in silico     121 CATTTGTTTCCGAGGATAT-GACCACGCAGTGCTTCAACTTATACAT-GCCTATGCTGAA 180


5770R-1.IR_full       181 TCAAGTGGCTCGCCACTTTCTCTGAGTTCCTATACAGGACTGCATACACCACCGCCAATG 240
                          |||||||||| ||||||||||| |||||||||| ||||||||||| ||||||||||||||
5770R-1.in silico     181 TCAAGTGGCT-GCCACTTTCTC-GAGTTCCTAT-CAGGACTGCAT-CACCACCGCCAATG 240


5770R-1.IR_full       241 CCCAGATCGCCAATTTGACCGCCCAGGCGAGAGCAGCAGCAGAAGACCTATCAGTCCGAC 300
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
5770R-1.in silico     241 CCCAGATCGCCAATTTGACCGCCCAGGCG-GAGCAGCAGCAGAAGACCTATCAGTCCGAC 300


5770R-1.IR_full       301 GTTTCAACACTCTGCAGTGCCTTTTACCGCCTGCGACAGCAACAATGATACCGCCAACTT 360
                          ||||||||||||||||||||| |||||||||||||||||||||||||||||||| |||||
5770R-1.in silico     301 GTTTCAACACTCTGCAGTGCC-TTTACCGCCTGCGACAGCAACAATGATACCGCAAACTT 360


5770R-1.IR_full       361 CTTCAACTGCTACGCGAATGCAGCTGATAATGACGTCTCTGTGATCTACACCCTGGCCAG 420
                          ||||||||||||||||||||| |||||||||||||||||||||||||||| |||||||||
5770R-1.in silico     361 CTTCAACTGCTACGCGAATGCTGCTGATAATGACGTCTCTGTGATCTACAACCTGGCCAG 420


5770R-1.IR_full       421 CAACGCTGCCAGCTCGGCCACCTCCTTCAGCTCGGGCATCCAAGCCATCCAGGACACCGA 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
5770R-1.in silico     421 CAACGCTGCCAGCTCGGCCACCTCCTTCAGCTCGGGCATCCAAGCCATCCAGGACACCGA 480


5770R-1.IR_full       481 GTACCAGTGCACCAACACAACGCAGAACAAC 511
                          |||||||||||||||||||||||||||||||
5770R-1.in silico     481 GTACCAGTGCACCAACACAACGCAGAACAAC 511



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137438.2  CG5770-RA (CG5770), mRNA 
4.56   22  39  74  125  NM_137439.1  CG5767-RA (CG5767), mRNA 
0   0  5  3  3  NM_080318.2  CG2715-RA (Syx4), mRNA 
0   0  5  2  3  NM_001042870.1  CG34126-RB (CG34126), mRNA 
0   0  4  3  7  NM_206501.1  CG3477-RA (Pxd), mRNA 
0   0  4  2  19  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
0   0  4  2  19  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
0   0  3  6  7  NM_132806.2  CG6340-RB, transcript variant B (CG6340), mRNA 
0   0  3  4  9  NM_132674.2  CG9938-RA (CG9938), mRNA 
0   0  3  4  5  NM_167449.1  CG6340-RD, transcript variant D (CG6340), mRNA 
0   0  3  2  4  NM_165996.1  CG6033-RD, transcript variant D (drk), mRNA 
0   0  2  5  6  NM_001042801.1  CG34104-RB, transcript variant B (CG34104), mRNA 
0   0  2  5  6  NM_001042802.1  CG34104-RA, transcript variant A (CG34104), mRNA 
0   0  2  5  3  NM_136557.2  CG8635-RA (CG8635), mRNA 
0   0  2  4  9  NM_168136.1  CG32418-RA (CG32418), mRNA 
0   0  2  3  8  NM_079309.2  CG5654-RA (yps), mRNA 
0   0  2  2  8  NM_169682.1  CG31291-RB, transcript variant B (CG31291), mRNA 
0   0  2  2  8  NM_169683.1  CG31291-RA, transcript variant A (CG31291), mRNA 
0   0  2  2  5  NM_143771.2  CG11546-RA, transcript variant A (kermit), mRNA 
0   0  2  2  5  NM_165590.1  CG11546-RB, transcript variant B (kermit), mRNA 
0   0  2  2  4  NM_139372.1  CG12104-RA (CG12104), mRNA 
0   0  1  6  8  NM_079861.2  CG1455-RA (CanA1), mRNA 
0   0  1  6  3  NM_139839.3  CG32381-RA (unc-13-4A), mRNA 
0   0  1  5  15  NM_169782.1  CG31243-RA, transcript variant A (cpo), mRNA 
0   0  1  5  15  NM_169781.1  CG31243-RE, transcript variant E (cpo), mRNA 
0   0  1  5  15  NM_169783.1  CG31243-RB, transcript variant B (cpo), mRNA 
0   0  1  5  14  NM_080105.2  CG31243-RF, transcript variant F (cpo), mRNA 
0   0  1  5  4  NM_078601.2  CG9533-RA (rut), mRNA 
0   0  1  5  4  NM_078746.4  CG11767-RA (Or24a), mRNA 
0   0  1  5  2  NM_001042901.1  CG5020-RG, transcript variant G (CLIP-190), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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