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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 4988R-3 
 Symbol CG4988  Full Name CG4988 
 CG No CG4988  Old CG No CG4988 
 Synonyms CG4988 
 Accession No (Link to NCBI) NM_135670.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTGGCCAGCGGTGGGTTTACA 
 Primer Seq. 3' 
aatctagaggtaccCCCTGGATCGGACAGTTCTCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTGGCCAGCG GTGGGTTTAC ATTGGATGAG ACTACAGTCA ACCTGACTAG GAACGTTGAG 
0061 GGCCAGTTTC ATCTGCACGG TGGATTCTTG GGATTCGACA GCGTCATTTG GGAAGTGGTG 
0121 CAGAAGACAC CACAGGGTGT TGTATTTCGC CATGAATCCC AACATGGTCA CGAGGGCTAT 
0181 CCAGGCATTC TAACGTGCCT TATCACCTAC CGGCTGGACA ATGAGGACCG TTTGTCCATT 
0241 AGTTTCGAGG CCACCACTGA CCGCCAGACA ATTGTCAATC TCTCGAACCA TGCCTATTTT 
0301 AATCTAGCTG GCCATAATGC AGGACCCAAA GGTCTTGCAG AGCATACGGT GGAGATAGCA 
0361 TCCAGTGAGA TTGTAGAAAC CGATGACCGG CAGATTCCAA CAGGGGAGTT AACCCTTGTT 
0421 GACAACACGG CNTTATGATC TCAGGTCACN TGTGGTCCTG GGAGATCGCC TCAAGCAGTT 
0481 TGAGAACTGT CCGATTCCAG Gg 
 in silico PCR Fragment 
0001 GTGGCCAGCG GTGGGTTTAC ATTGGATGAG ACTACAGTAA ACCTGACTAG GAACGTTGAG 
0061 GGCCAGTTTC ATCTGCACGG TGGATTCTTG GGATTCGACA GCGTCATTTG GGAAGTGGTG 
0121 CAGAAGACAC CACAGGGTGT TGTATTTCGC CATGAATCCC AACATGGTCA CGAGGGCTAT 
0181 CCAGGCATTC TAACGTGCCT TATCACCTAT CGGCTGGACA ATGAGGACCG TTTGTCCATT 
0241 AGTTTCGAGG CCACCACTGA CCGCCAGACA ATTGTCAATC TCTCGAACCA TGCCTATTTT 
0301 AATCTAGCTG GCCATAATGC AGGACCCAAA GGTCTTGCAG AGCATACGGT GGAGATAGCA 
0361 TCCAGTGAGA TTGTAGAAAC GGATGACCGG CAGATTCCAA CAGGGGAGTT AACCCTTGTT 
0421 GACAACACGG C-TTATGATC TCAGGTCACC TGTGGTCCTG GGAGATCGCC TCAAGCAGTT 
0481 TGAGAACTGT CCGA-TCCAG GG 
 Assemble Data 

4988R-3.IR_full        1   TGGCCAGCGGTGGGTTTACATTGGATGAGACTACAGTCAACCTGACTAGGAACGTTGAG 59
                           ||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
4988R-3.in silico      1   TGGCCAGCGGTGGGTTTACATTGGATGAGACTACAGTAAACCTGACTAGGAACGTTGAG 59


4988R-3.IR_full       61  GGCCAGTTTCATCTGCACGGTGGATTCTTGGGATTCGACAGCGTCATTTGGGAAGTGGTG 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4988R-3.in silico     61  GGCCAGTTTCATCTGCACGGTGGATTCTTGGGATTCGACAGCGTCATTTGGGAAGTGGTG 119


4988R-3.IR_full       121 CAGAAGACACCACAGGGTGTTGTATTTCGCCATGAATCCCAACATGGTCACGAGGGCTAT 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4988R-3.in silico     121 CAGAAGACACCACAGGGTGTTGTATTTCGCCATGAATCCCAACATGGTCACGAGGGCTAT 179


4988R-3.IR_full       181 CCAGGCATTCTAACGTGCCTTATCACCTACCGGCTGGACAATGAGGACCGTTTGTCCATT 239
                          ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
4988R-3.in silico     181 CCAGGCATTCTAACGTGCCTTATCACCTATCGGCTGGACAATGAGGACCGTTTGTCCATT 239


4988R-3.IR_full       241 AGTTTCGAGGCCACCACTGACCGCCAGACAATTGTCAATCTCTCGAACCATGCCTATTTT 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4988R-3.in silico     241 AGTTTCGAGGCCACCACTGACCGCCAGACAATTGTCAATCTCTCGAACCATGCCTATTTT 299


4988R-3.IR_full       301 AATCTAGCTGGCCATAATGCAGGACCCAAAGGTCTTGCAGAGCATACGGTGGAGATAGCA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4988R-3.in silico     301 AATCTAGCTGGCCATAATGCAGGACCCAAAGGTCTTGCAGAGCATACGGTGGAGATAGCA 359


4988R-3.IR_full       361 TCCAGTGAGATTGTAGAAACCGATGACCGGCAGATTCCAACAGGGGAGTTAACCCTTGTT 419
                          |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
4988R-3.in silico     361 TCCAGTGAGATTGTAGAAACGGATGACCGGCAGATTCCAACAGGGGAGTTAACCCTTGTT 419


4988R-3.IR_full       421 GACAACACGGCNTTATGATCTCAGGTCACNTGTGGTCCTGGGAGATCGCCTCAAGCAGTT 479
                          ||||||||||| ||||||||||||||||| ||||||||||||||||||||||||||||||
4988R-3.in silico     421 GACAACACGGC-TTATGATCTCAGGTCACCTGTGGTCCTGGGAGATCGCCTCAAGCAGTT 479


4988R-3.IR_full       481 TGAGAACTGTCCGATTCCAGGG 501
                          |||||||||||||| |||||||
4988R-3.in silico     481 TGAGAACTGTCCGA-TCCAGGG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135670.1  CG4988-RA (CG4988), mRNA 
0   0  4  21  7  NM_168931.1  CG32445-RA (CG32445), mRNA 
0   0  2  38  78  NM_168930.1  CG32444-RA (CG32444), mRNA 
0   0  1  4  2  NM_142549.1  CG11626-RA (CG11626), mRNA 
0   0  1  3  2  NM_169374.1  CG31299-RA, transcript variant A (nocturnin), mRNA 
0   0  1  2  5  NM_137267.1  CG9068-RA (CG9068), mRNA 
0   0  1  2  4  NM_136834.1  CG13204-RA, transcript variant A (CG13204), mRNA 
0   0  1  2  4  NM_206363.2  CG17839-RB, transcript variant B (CG17839), mRNA 
0   0  1  2  3  NM_170264.1  CG6058-RD, transcript variant D (Ald), mRNA 
0   0  1  2  3  NM_206404.1  CG33287-RA (CG33287), mRNA 
0   0  1  2  3  NM_170261.1  CG6058-RF, transcript variant F (Ald), mRNA 
0   0  1  2  3  NM_170263.1  CG6058-RC, transcript variant C (Ald), mRNA 
0   0  1  2  3  NM_170262.1  CG6058-RB, transcript variant B (Ald), mRNA 
0   0  1  2  2  NM_206577.1  CG6058-RH, transcript variant H (Ald), mRNA 
0   0  1  2  2  NM_170266.2  CG6058-RG, transcript variant G (Ald), mRNA 
0   0  1  2  2  NM_001043141.1  CG17839-RC, transcript variant C (CG17839), mRNA 
0   0  1  2  2  NM_170265.2  CG6058-RA, transcript variant A (Ald), mRNA 
0   0  1  2  2  NM_132621.1  CG12715-RA (CG12715), mRNA 
0   0  1  2  2  NM_079791.3  CG6058-RE, transcript variant E (Ald), mRNA 
0   0  1  2  2  NM_057374.2  CG3722-RA (shg), mRNA 
0   0  1  2  2  NM_169098.2  CG2095-RA (CG2095), mRNA 
0   0  0  7  5  NM_132245.1  CG1387-RA (CG1387), mRNA 
0   0  0  6  12  NM_139769.1  CG10467-RA (CG10467), mRNA 
0   0  0  4  7  NM_141677.2  CG8383-RA (Pnn), mRNA 
0   0  0  4  3  NM_140377.1  CG17687-RA (CG17687), mRNA 
0   0  0  4  3  NM_137449.2  CG5661-RA (Sema-5c), mRNA 
0   0  0  4  3  NM_136802.1  CG13227-RA (CG13227), mRNA 
0   0  0  3  8  NM_167753.1  CG1417-RB, transcript variant B (slgA), mRNA 
0   0  0  3  8  NM_167752.1  CG1417-RD, transcript variant D (slgA), mRNA 
0   0  0  3  5  NM_078688.2  CG14226-RA (dome), mRNA 

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