NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4960R-3

Symbol CG4960 Full Name CG4960

CG No CG4960 Old CG No CG4960

Synonyms BcDNA:AT31258, CG4960

Accession No (Link to NCBI)

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCCCAGGTAAGTCAGGTGCAG

Primer Seq. 3'

aatctagaggtaccCCAGCGGACATCATGTCGACG

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


4960R-3.IR full       1   --CCAGGTAAATCAGGTGCAGCGGTTCTTCAACGGCTACAAGCAGGACATTAACCGATCA 60
                            |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     1   GCCCAGGTAAGTCAGGTGCAGCGGTTCTTCAACGGCTACAAGCAGGACATTAACCGATCA 60


4960R-3.IR full       61  CTGCGCGATGCGTCCAAGCCGTGGACCAAAGCCTTTGATAGCCTGGAAGAGAAGACCGGT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     61  CTGCGCGATGCGTCCAAGCCGTGGACCAAAGCCTTTGATAGCCTGGAAGAGAAGACCGGT 120


4960R-3.IR full       121 GTGGAGAGGGTGAATATTTTCGTGGGTAGTATTTTGTTTTGCGCTTTCTACCTGGTCTTT 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     121 GTGGAGAGGGTGAATATTTTCGTGGGTAGTATTTTGTTTTGCGCTTTCTACCTGGTCTTT 180


4960R-3.IR full       181 GGCTGGTGCGCTCAGCTACTGTGCAACATCATTGGGGTTCTTTACCCTGCTTACATTTCC 240
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     181 GGCTGGTGCGCTCAGCTATTGTGCAACATCATTGGGGTTCTTTACCCTGCTTACATTTCC 240


4960R-3.IR full       241 ATCCATGGCATCGAGTCCAGTACGAAGCAGGACGACATCAGGTGGCTGATCTACTGGGTC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     241 ATCCATGGCATCGAGTCCAGTACGAAGCAGGACGACATCAGGTGGCTGATCTACTGGGTC 300


4960R-3.IR full       301 ACTTTTGGCATCTTCACTGTGATTGAATTCTATCCAAGTCTGCTAACTTCGATGATTCCC 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4960R-3.in silico     301 ACTTTTGGCATCTTCACTGTGATTGAATTCTATCCAAGTCTGCTAACTTCGATGATTCCC 360


4960R-3.IR full       361 TTCTACTGGCTGTTGAAGTGTACTTTCCTCATCTGGTGNATGCTGCCCACCGAACGGAAT 420
                          |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
4960R-3.in silico     361 TTCTACTGGCTGTTGAAGTGTACTTTCCTCATCTGGTGCATGCTGCCCACCGAACGGAAT 420


4960R-3.IR full       421 GGTTCTACCCTTATCTACCACAAGCTGGTCCGACCCTACTTCCTGAAGCTTCACGATCCC 480
                          ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
4960R-3.in silico     421 GGTTCTACCCTTATCTACCACAAGCTGGTCCGACCATACTTCCTGAAGCTTCACGATCCC 480


4960R-3.IR full       481 GTCGACATGATGTNCGCTGG 500
                          ||||||||||||| ||||||
4960R-3.in silico     481 GTCGACATGATGTCCGCTGG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc