NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4791R-1

Symbol CG33301 Full Name CG33301

CG No CG33301 Old CG No CG4791

Synonyms CG4791, CG33301

Accession No (Link to NCBI) NM_205955.3

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

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Reference
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Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGCTAACCGCGGCGTATTTG

Primer Seq. 3'

aatctagaggtaccCGTTCCTGCAGAACTATGGAG

Predicted Fragment Size

IR fragment full Seq

0001 TGGCTAACCG CGGCGTATTT GCAGCCCAGA CTAAGGGCAT ACTGCCAGGA TGACCGGCTG 
0061 CAGGTCCTTA GAATCTGGGC CAAGCCANCG ACGGGGAAAG GAGAGAACTT TGTTGGCGTG 
0121 ATGAcCCGCA TCTATGTGGA CTATCAGCTG GGCGATGGAT CGGTTGTGAA TAAGACCTAC 
0181 ATAGTGAAAC AGGCTCTGTC CGCCGAAGTT NCCCCAGGCG GAGGTCTTCT TCGAATATGA 
0241 GCTGTACACC CGTGAAATGG ACATGTACGA GTTCATTCTG CCCAAGTTGA AGGAACTGCT 
0301 GCAAGAAGCT GGGCTGGACC AAAAACTAAC TGCAGATGCC ATCACCGTGG ACCGCGAGTA 
0361 CAACACCATG ATTCTGGAGG ATTTGGCACC GTACAAGTTC GTCAATNNGG ATCGCGTGAA 
0421 GCAGCTGGAC ATGGCACACA CCGAGCTGAC ACTGGAAATG CTGGCCAAAT TCCATGCCGC 
0481 CTCCATAGTT CTGCAGGAAC G

in silico PCR Fragment

0001 TGGCTAACCG CGGCGTATTT GCAGCCCAGA CTAAGGGCAT ACTGCCAGGA TGACCGGCTG 
0061 CAGGTCCTTA GAATCTGGGC CAAGCCAGCG ACGGGGAAAG GAGAGAACTT TGTTGGCGTG 
0121 ATGACCCGCA TCTATGTGGA CTATCAGCTG GGCGATGGAT CGGTTGTGAA TAAGACCTAC 
0181 ATAGTGAAAC AGGCTCTGTC CGCCGAAGTT -CCCCAGGCG GAGGTCTTTT TCGAATATGA 
0241 GCTGTACACT CGTGAAATGG ACATGTACGA GTTCATTCTG CCCAAGTTGA AGGAACTGCT 
0301 GCAAGAAGCT GGGCTGGACC AAAAACTAAC TGCAGATGCC ATCACCGTGG ACCGCGAGTA 
0361 CAACACCATG ATTCTGGAGG ATTTGGCACC GTACAAGTTC GTCAATGCGG ATCGCGTGAA 
0421 GCAGCTGGAC ATGGCACACA CCGAGCTGAC ACTGGAAATG CTGGCCAAAT TCCATGCCGC 
0481 CTCCATAGTT CTGCAGGAAC G

Assemble Data


4791R-1.IR_full       1   TGGCTAACCGCGGCGTATTTGCAGCCCAGACTAAGGGCATACTGCCAGGATGACCGGCTG 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4791R-1.in silico     1   TGGCTAACCGCGGCGTATTTGCAGCCCAGACTAAGGGCATACTGCCAGGATGACCGGCTG 60


4791R-1.IR_full       61  CAGGTCCTTAGAATCTGGGCCAAGCCANCGACGGGGAAAGGAGAGAACTTTGTTGGCGTG 120
                          ||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||
4791R-1.in silico     61  CAGGTCCTTAGAATCTGGGCCAAGCCAGCGACGGGGAAAGGAGAGAACTTTGTTGGCGTG 120


4791R-1.IR_full       121 ATGACCCGCATCTATGTGGACTATCAGCTGGGCGATGGATCGGTTGTGAATAAGACCTAC 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4791R-1.in silico     121 ATGACCCGCATCTATGTGGACTATCAGCTGGGCGATGGATCGGTTGTGAATAAGACCTAC 180


4791R-1.IR_full       181 ATAGTGAAACAGGCTCTGTCCGCCGAAGTTNCCCCAGGCGGAGGTCTTCTTCGAATATGA 240
                          |||||||||||||||||||||||||||||| ||||||||||||||||| |||||||||||
4791R-1.in silico     181 ATAGTGAAACAGGCTCTGTCCGCCGAAGTT-CCCCAGGCGGAGGTCTTTTTCGAATATGA 240


4791R-1.IR_full       241 GCTGTACACCCGTGAAATGGACATGTACGAGTTCATTCTGCCCAAGTTGAAGGAACTGCT 300
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
4791R-1.in silico     241 GCTGTACACTCGTGAAATGGACATGTACGAGTTCATTCTGCCCAAGTTGAAGGAACTGCT 300


4791R-1.IR_full       301 GCAAGAAGCTGGGCTGGACCAAAAACTAACTGCAGATGCCATCACCGTGGACCGCGAGTA 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4791R-1.in silico     301 GCAAGAAGCTGGGCTGGACCAAAAACTAACTGCAGATGCCATCACCGTGGACCGCGAGTA 360


4791R-1.IR_full       361 CAACACCATGATTCTGGAGGATTTGGCACCGTACAAGTTCGTCAATNNGGATCGCGTGAA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||  ||||||||||||
4791R-1.in silico     361 CAACACCATGATTCTGGAGGATTTGGCACCGTACAAGTTCGTCAATGCGGATCGCGTGAA 420


4791R-1.IR_full       421 GCAGCTGGACATGGCACACACCGAGCTGACACTGGAAATGCTGGCCAAATTCCATGCCGC 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4791R-1.in silico     421 GCAGCTGGACATGGCACACACCGAGCTGACACTGGAAATGCTGGCCAAATTCCATGCCGC 480


4791R-1.IR_full       481 CTCCATAGTTCTGCAGGAACG 501
                          |||||||||||||||||||||
4791R-1.in silico     481 CTCCATAGTTCTGCAGGAACG 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_205955.3	CG33301-RA (CG33301), mRNA
0.41	2	4	3	4	NM_143114.1	CG10514-RA (CG10514), mRNA
0	0	10	19	49	NM_137608.2	CG16898-RA (CG16898), mRNA
0	0	7	11	13	NM_143112.1	CG11878-RA (CG11878), mRNA
0	0	5	5	2	NM_134800.1	CG12674-RA (CG12674), mRNA
0	0	2	6	9	NM_143609.2	CG31002-RA (CG31002), mRNA
0	0	2	5	3	NM_057842.2	CG16858-RA (vkg), mRNA
0	0	2	4	3	NM_165975.1	CG4712-RA, transcript variant A (CG4712), mRNA
0	0	2	4	3	NM_137012.1	CG4712-RB, transcript variant B (CG4712), mRNA
0	0	1	5	2	NM_130486.1	CG3176-RA (CG3176), mRNA
0	0	1	5	2	NM_166842.1	CG32817-RA (CG32817), mRNA
0	0	1	3	9	NM_166790.2	CG32019-RA, transcript variant A (bt), mRNA
0	0	1	3	9	NM_205877.1	CG32019-RD, transcript variant D (bt), mRNA
0	0	1	3	9	NM_205876.1	CG32019-RC, transcript variant C (bt), mRNA
0	0	1	3	9	NM_205875.1	CG32019-RE, transcript variant E (bt), mRNA
0	0	1	3	4	NM_139619.1	CG1332-RA (CG1332), mRNA
0	0	1	3	4	NM_001014640.1	CG14299-RB, transcript variant B (CG14299), mRNA
0	0	1	3	4	NM_142495.1	CG14299-RA, transcript variant A (CG14299), mRNA
0	0	1	3	3	NM_167806.1	CG16940-RB, transcript variant B (CG16940), mRNA
0	0	1	3	3	NM_167805.1	CG16940-RC, transcript variant C (CG16940), mRNA
0	0	1	3	3	NM_138168.2	CG16940-RA, transcript variant A (CG16940), mRNA
0	0	1	3	3	NM_134481.1	CG14220-RA (CG14220), mRNA
0	0	1	3	3	NM_138070.2	CG3492-RA (CG3492), mRNA
0	0	1	2	2	NM_168068.1	CG32252-RA (CG32252), mRNA
0	0	1	2	2	NM_166430.1	CG9441-RB, transcript variant B (Pu), mRNA
0	0	0	26	23	NM_143110.2	CG11889-RA, transcript variant A (CG11889), mRNA
0	0	0	26	23	NM_143111.2	CG11891-RA, transcript variant A (CG11891), mRNA
0	0	0	20	31	NM_169129.1	CG31562-RA (CG31562), mRNA
0	0	0	17	11	NM_001032041.1	CG11891-RB, transcript variant B (CG11891), mRNA
0	0	0	14	12	NM_001032040.1	CG11891-RC, transcript variant C (CG11891), mRNA

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