NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4375R-4

Symbol CG4375 Full Name CG4375

CG No CG4375 Old CG No CG4375

Synonyms BcDNA:AT13819, CG4375

Accession No (Link to NCBI) NM_164402.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGATTTAAAACCCGCGTCCTTC

Primer Seq. 3'

aatctagaggtaccAAGGCACTACTGCGACTATGA

Predicted Fragment Size

IR fragment full Seq

0001 NATTTAAAAC CCGCGTCCTT CGAAGACTTG GCCAATCAGG TGTTCGAAAA GTCGAAACAG 
0061 ATGAGTATGT CAATGAAGGA GCAGTGCAAG TTGGTCCAGG AGTATCTGAA GGCTAGGCTC 
0121 GATGGGAGTG ATGGAANGCG ATGCAGAATA TAATAGTGAT TCCTATTCAG ATTTTGAATC 
0181 CGAGTCGGAT TCCGATTCCG ATTCCGATTT CGAGGAGGAT TCCCTTACTG ATTCCGATGA 
0241 GCAAACAGAN GAGAGCAGNG ATGAGTCCGA ATCGGAACAC AGTGAAGTCG AGGAGCGTAC 
0301 CAAACCATCA GATACTCACA AGACTACCAA CGGAGGTCTC TGCGAAAAAC CAATCTCCAA 
0361 GGTCGAGCCT GAACAATCNT CGGCTCGTGG CCTTTGTGGC TCCTCTCAAG TCCCCTGCCC 
0421 CCCNCATCCA CCGNCGACAT GACTATGGCT GGTGGCGTTA TAgCTCCTCA TAGTCGCAGT 
0481 AGTGCCTT

in silico PCR Fragment

0001 GATTTAAAAC CCGCGTCCTT CGAAGACTTG GCCAATCAGG TGTTCGAAAA GTCGAAACAG 
0061 ATGAGTATGT CAATGAAGGA GCAGTGCAAG TTGGTCCAGG AGTATTTGAA AGCTAGGCTC 
0121 GATGGGAGTG ATGGAA-GCG ATGCAGAATA TAATAGTGAT TCCTATTCAG ATTTTGAATC 
0181 CGAGTCGGAT TCCGATTCCG ATTCCGATTT CGAGGAGGAT TCCCTTACTG ATTCCGATGA 
0241 GCAAACAGAG GAGAGCAGTG ATGAGTCCGA ATCGGAACAC AGTGAAGTCG AGGAGCGTAC 
0301 CAAACCATCA GATACTCACA AGACTACCAA CGGAGGTCTC TGCGAAAAAC CAATCTCCAA 
0361 GGTCGAGCCT GAACAATC-T CGGCTCGTGG CCTTTGTGGC TCCTCTCAAG TCCCCTGCCC 
0421 CCCGCATCCA CCGTCGAC-T GACTATGGCT GGTGGCGTTA TAGCTCCTCA TAGTCGCAGT 
0481 AGTGCCTT

Assemble Data


4375R-4.IR_full        1   ATTTAAAACCCGCGTCCTTCGAAGACTTGGCCAATCAGGTGTTCGAAAAGTCGAAACAG 59
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico      1   ATTTAAAACCCGCGTCCTTCGAAGACTTGGCCAATCAGGTGTTCGAAAAGTCGAAACAG 59


4375R-4.IR_full       61  ATGAGTATGTCAATGAAGGAGCAGTGCAAGTTGGTCCAGGAGTATCTGAAGGCTAGGCTC 119
                          ||||||||||||||||||||||||||||||||||||||||||||| |||| |||||||||
4375R-4.in silico     61  ATGAGTATGTCAATGAAGGAGCAGTGCAAGTTGGTCCAGGAGTATTTGAAAGCTAGGCTC 119


4375R-4.IR_full       121 GATGGGAGTGATGGAANGCGATGCAGAATATAATAGTGATTCCTATTCAGATTTTGAATC 179
                          |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     121 GATGGGAGTGATGGAA-GCGATGCAGAATATAATAGTGATTCCTATTCAGATTTTGAATC 179


4375R-4.IR_full       181 CGAGTCGGATTCCGATTCCGATTCCGATTTCGAGGAGGATTCCCTTACTGATTCCGATGA 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     181 CGAGTCGGATTCCGATTCCGATTCCGATTTCGAGGAGGATTCCCTTACTGATTCCGATGA 239


4375R-4.IR_full       241 GCAAACAGANGAGAGCAGNGATGAGTCCGAATCGGAACACAGTGAAGTCGAGGAGCGTAC 299
                          ||||||||| |||||||| |||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     241 GCAAACAGAGGAGAGCAGTGATGAGTCCGAATCGGAACACAGTGAAGTCGAGGAGCGTAC 299


4375R-4.IR_full       301 CAAACCATCAGATACTCACAAGACTACCAACGGAGGTCTCTGCGAAAAACCAATCTCCAA 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     301 CAAACCATCAGATACTCACAAGACTACCAACGGAGGTCTCTGCGAAAAACCAATCTCCAA 359


4375R-4.IR_full       361 GGTCGAGCCTGAACAATCNTCGGCTCGTGGCCTTTGTGGCTCCTCTCAAGTCCCCTGCCC 419
                          |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     361 GGTCGAGCCTGAACAATC-TCGGCTCGTGGCCTTTGTGGCTCCTCTCAAGTCCCCTGCCC 419


4375R-4.IR_full       421 CCCNCATCCACCGNCGACATGACTATGGCTGGTGGCGTTATAGCTCCTCATAGTCGCAGT 479
                          ||| ||||||||| |||| |||||||||||||||||||||||||||||||||||||||||
4375R-4.in silico     421 CCCGCATCCACCGTCGAC-TGACTATGGCTGGTGGCGTTATAGCTCCTCATAGTCGCAGT 479


4375R-4.IR_full       481 AGTGCCTT 487
                          ||||||||
4375R-4.in silico     481 AGTGCCTT 487

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	467	6	14	35	NM_164402.1	CG4375-RA (CG4375), mRNA
0.85	4	17	17	18	NM_169432.2	CG31116-RA, transcript variant A (CG31116), mRNA
0.85	4	17	17	18	NM_169431.2	CG31116-RC, transcript variant C (CG31116), mRNA
0.85	4	15	57	62	NM_167339.1	CG32647-RB, transcript variant B (CG32647), mRNA
0.85	4	15	57	62	NM_167338.1	CG32647-RA, transcript variant A (CG32647), mRNA
0.64	3	18	35	43	NM_137751.3	CG10082-RB, transcript variant B (CG10082), mRNA
0.64	3	13	18	22	NM_001043290.1	CG34100-RB, transcript variant B (mld), mRNA
0.64	3	7	16	20	NM_132058.2	CG5966-RA (CG5966), mRNA
0.42	2	3	5	8	NM_135796.2	CG9377-RA (CG9377), mRNA
0.42	2	3	2	19	NM_168352.1	CG32048-RA, transcript variant A (CG32048), mRNA
0.21	1	3	13	16	NM_132122.2	CG3126-RA, transcript variant A (C3G), mRNA
0.21	1	3	13	15	NM_176694.1	CG3126-RB, transcript variant B (C3G), mRNA
0	0	13	20	31	NM_206654.1	CG18009-RA, transcript variant A (Trf2), mRNA
0	0	13	20	31	NM_078529.3	CG18009-RD, transcript variant D (Trf2), mRNA
0	0	7	15	31	NM_168505.1	CG10601-RB, transcript variant B (mirr), mRNA
0	0	7	15	31	NM_079323.2	CG10601-RA, transcript variant A (mirr), mRNA
0	0	7	13	27	NM_140315.3	CG4357-RA, transcript variant A (Ncc69), mRNA
0	0	5	6	14	NM_135175.2	CG9505-RA (CG9505), mRNA
0	0	5	6	10	NM_130542.2	CG11403-RA (CG11403), mRNA
0	0	4	16	17	NM_166865.2	CG32815-RA (CG32815), mRNA
0	0	4	12	19	NM_167598.1	CG32552-RA (CG32552), mRNA
0	0	4	7	17	NM_170659.1	CG7835-RA (CG7835), mRNA
0	0	3	10	21	NM_141823.2	CG5308-RA, transcript variant A (dpr5), mRNA
0	0	3	9	17	NM_135302.3	CG7191-RA (CG7191), mRNA
0	0	3	2	5	NM_080081.2	CG11450-RA (net), mRNA
0	0	3	2	2	NM_139893.2	CG7457-RA (CG7457), mRNA
0	0	2	17	23	NM_136984.3	CG13322-RA, transcript variant A (CG13322), mRNA
0	0	2	17	23	NM_165962.2	CG13322-RB, transcript variant B (CG13322), mRNA
0	0	2	17	23	NM_165963.1	CG13322-RC, transcript variant C (CG13322), mRNA
0	0	2	11	14	NM_143392.2	CG14521-RA (CG14521), mRNA

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