NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

32371R-1

Symbol

CG32371

Full Name

CG32371

CG No

CG32371

Old CG No

CG32371

Synonyms

EB1, BEST:GH23475, CG32371

Accession No (Link to NCBI)

NM_168231.1

Inserted Chr.

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCAGTTTTTCAAGGTCGAGGA

Primer Seq. 3'

aatctagaggtaccATCTGTTTGAGCAGAGGCTTC

Predicted Fragment Size

IR fragment full Seq

0001 GCAGTTTTTC AAGGTCGAGG AATTGTGCAC CGGAGCGGCA TACTGCCAGT TAATGGACAT 
0061 ACTGTTTGCC CGGTCGATCC CGATGCAGCG AGTCAAGTTT CGCACAAACG TTGAGTATGA 
0121 GTACATCCAG AATTTCAAGC TTTTACAGGG ATGTTTCAAC AAATTTGTTG TTGATAAGAT 
0181 CATTCCCATC GACCGGCTGG TCAAGGGACG ATACCAGGAT AACTTCGAGT TCCTTCAGTG 
0241 GTTTCGCAAG TTCTTCGATG CCAACTATGA GAGTCGAGAA TATGATCCTG TGATAGCTCG 
0301 GAATGGAGCA ATGCTGGGAC TTGGCTCGCC TCCCATGGAA GCCAAGTTAC GCAAATCAGT 
0361 GAATAAGTCA AATCCCCAAA CTAAACCCAC CGAAGAATCC TCTGCTCAAA CAGATAGGGC 
0421 AACAACAGAA CCGAAAAATC AAGTGTATAA GTCAAATTCT GAAAATAAAA CCATCGAAGA 
0481 AGCCTCTGCT CAAACAGAT

in silico PCR Fragment

0001 GCAGTTTTTC AAGGTCGAGG AATTGTGCAC CGGAGCGGCA TACTGCCAGT TAATGGACAT 
0061 ACTGTTTGCC CGGTCGATCC CGATGCAGCG AGTCAAGTTT CGCACAAACG TTGAGTATGA 
0121 GTACATCCAG AATTTCAAGC TTTTACAGGG ATGTTTCAAC AAATTTGTTG TTGATAAGAT 
0181 CATTCCCATC GACCGGCTGG TCAAGGGACG ATACCAGGAT AACTTCGAGT TCCTTCAGTG 
0241 GTTTCGCAAG TTCTTCGATG CCAACTATGA GAGTCGAGAA TATGATCCTG TGATAGCTCG 
0301 GAATGGAGCA ATGCTGGGAC TTGGCTCGCC TCCCATGGAA GCCAAGTTAC GCAAATCAGT 
0361 GAATAAGTCA AATCCCCAAA CTAAACCCAC CGAAGAATCC TCTGCTCAAA CAGATAGGGC 
0421 AACAACAGAA CCGAAAAATC AAGTGTATAA GTCAAATTCT GAAAATAAAA CCATCGAAGA 
0481 AGCCTCTGCT CAAACAGAT

Assemble Data


32371R-1.IR_full       1   GCAGTTTTTCAAGGTCGAGGAATTGTGCACCGGAGCGGCATACTGCCAGTTAATGGACAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     1   GCAGTTTTTCAAGGTCGAGGAATTGTGCACCGGAGCGGCATACTGCCAGTTAATGGACAT 60


32371R-1.IR_full       61  ACTGTTTGCCCGGTCGATCCCGATGCAGCGAGTCAAGTTTCGCACAAACGTTGAGTATGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     61  ACTGTTTGCCCGGTCGATCCCGATGCAGCGAGTCAAGTTTCGCACAAACGTTGAGTATGA 120


32371R-1.IR_full       121 GTACATCCAGAATTTCAAGCTTTTACAGGGATGTTTCAACAAATTTGTTGTTGATAAGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     121 GTACATCCAGAATTTCAAGCTTTTACAGGGATGTTTCAACAAATTTGTTGTTGATAAGAT 180


32371R-1.IR_full       181 CATTCCCATCGACCGGCTGGTCAAGGGACGATACCAGGATAACTTCGAGTTCCTTCAGTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     181 CATTCCCATCGACCGGCTGGTCAAGGGACGATACCAGGATAACTTCGAGTTCCTTCAGTG 240


32371R-1.IR_full       241 GTTTCGCAAGTTCTTCGATGCCAACTATGAGAGTCGAGAATATGATCCTGTGATAGCTCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     241 GTTTCGCAAGTTCTTCGATGCCAACTATGAGAGTCGAGAATATGATCCTGTGATAGCTCG 300


32371R-1.IR_full       301 GAATGGAGCAATGCTGGGACTTGGCTCGCCTCCCATGGAAGCCAAGTTACGCAAATCAGT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     301 GAATGGAGCAATGCTGGGACTTGGCTCGCCTCCCATGGAAGCCAAGTTACGCAAATCAGT 360


32371R-1.IR_full       361 GAATAAGTCAAATCCCCAAACTAAACCCACCGAAGAATCCTCTGCTCAAACAGATAGGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     361 GAATAAGTCAAATCCCCAAACTAAACCCACCGAAGAATCCTCTGCTCAAACAGATAGGGC 420


32371R-1.IR_full       421 AACAACAGAACCGAAAAATCAAGTGTATAAGTCAAATTCTGAAAATAAAACCATCGAAGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
32371R-1.in silico     421 AACAACAGAACCGAAAAATCAAGTGTATAAGTCAAATTCTGAAAATAAAACCATCGAAGA 480


32371R-1.IR_full       481 AGCCTCTGCTCAAACAGAT 499
                           |||||||||||||||||||
32371R-1.in silico     481 AGCCTCTGCTCAAACAGAT 499

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100.2	482	23	44	63	NM_168231.1	CG32371-RA (CG32371), mRNA
0.2	1	3	3	4	NM_166606.1	CG30181-RA (CG30181), mRNA
0	0	2	24	40	NM_165487.2	CG3265-RC, transcript variant C (Eb1), mRNA
0	0	2	13	32	NM_136389.3	CG3265-RB, transcript variant B (Eb1), mRNA
0	0	2	13	32	NM_206030.1	CG3265-RE, transcript variant E (Eb1), mRNA
0	0	2	13	32	NM_165489.1	CG3265-RD, transcript variant D (Eb1), mRNA
0	0	2	13	32	NM_165488.1	CG3265-RA, transcript variant A (Eb1), mRNA
0	0	2	4	3	NM_164685.1	CG31642-RA (CG31642), mRNA
0	0	2	2	4	NM_079594.2	CG5242-RA (mRpL40), mRNA
0	0	2	2	3	NM_134758.2	CG7420-RA (CG7420), mRNA
0	0	2	2	2	NM_176359.1	CG9614-RK, transcript variant K (pip), mRNA
0	0	1	8	7	NM_078670.2	CG7092-RA (Dhc16F), mRNA
0	0	1	4	3	NM_164943.1	CG6720-RB, transcript variant B (UbcD2), mRNA
0	0	1	4	3	NM_057789.3	CG6720-RA, transcript variant A (UbcD2), mRNA
0	0	1	4	2	NM_138050.2	CG3363-RA (CG3363), mRNA
0	0	1	3	3	NM_130596.2	CG14804-RA (CG14804), mRNA
0	0	1	3	2	NM_142630.1	CG10881-RA (CG10881), mRNA
0	0	1	2	6	NM_079355.2	CG6988-RA, transcript variant A (Pdi), mRNA
0	0	1	2	6	NM_168613.1	CG6988-RB, transcript variant B (Pdi), mRNA
0	0	1	2	6	NM_168614.2	CG6988-RD, transcript variant D (Pdi), mRNA
0	0	1	2	3	NM_140251.2	CG10907-RA (CG10907), mRNA
0	0	1	2	2	NM_169140.1	CG10277-RC, transcript variant C (CG10277), mRNA
0	0	1	2	2	NM_164959.1	CG6320-RB, transcript variant B (Ca-beta), mRNA
0	0	1	2	2	NM_134726.2	CG4715-RA (Iris), mRNA
0	0	1	2	2	NM_205962.1	CG6320-RD, transcript variant D (Ca-beta), mRNA
0	0	1	2	2	NM_205963.1	CG6320-RC, transcript variant C (Ca-beta), mRNA
0	0	1	2	2	NM_078822.2	CG6320-RA, transcript variant A (Ca-beta), mRNA
0	0	0	8	15	NM_137540.1	CG18190-RA (CG18190), mRNA
0	0	0	5	4	NM_142354.1	CG4090-RA (CG4090), mRNA
0	0	0	5	3	NM_080362.2	CG31868-RA (Samuel), mRNA

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