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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31817R-4 
 Symbol CG31817  Full Name CG31817 
 CG No CG31817  Old CG No CG31817 
 Synonyms DS02740.18, BG:DS02740.18, anon-WO03042407.99, anon-WO03042407.98, CG31817 
 Accession No (Link to NCBI) NM_165153.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCAGCTCTCGATCGCGAAATA 
 Primer Seq. 3' 
aatctagaggtaccAATTTCCGTTTCCGAGGGACT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCAGCTCTCG ATCGCGAAAT AGTACCAGCA ATTACCCATC CACGTGGCAA CTTAACATCA 
0061 GCAGAGCTAA AGAGGAACTG AAAAAGGTTT CTAAAGGATC GCTGGACGAG ATTATCAATT 
0121 GTTCGATTCT CAATCGTACC CCTTATGATC ATCAACTTAA TACGCCCCTG AAGTCGGATA 
0181 ATTCCTGTCA GTGCACTATC GACAAGAAGG CTAGAAAACG CTTCAAGAAA AACAAAGCGG 
0241 TTTCGGTGGC CGAAACCCAG ACAATCAGCG AAGATGTGAC AAGGAATTAC ACTGAAGATA 
0301 TGAAACCTCC AGCTCCAGAG GGATTTCAGA AATTGAAGAA GAGGGTCAAC AGCGGGGAGG 
0361 AATATAAGCC ACAGCGAAAG GTTTCCAAAA CTCCATCAAG AAGTAGCGGA AACTCTACGG 
0421 ATTTTTGTAG ATGTAATCAA CACATTTCAG GACCAACTTA CAAGGACTAT GGGGGTTATA 
0481 GTCCCTCGGA AACGGAAATT  
 in silico PCR Fragment 
0001 GCAGCTCTCG ATCGCGAAAT AGTACCAGCA ATTACCCATC CACGTGGCAA CTTAACATCA 
0061 GCAGAGCTAA AGAGGAACTG AAAAAGGTTT CTAAAGGATC GCTGGACGAG ATTATCAATT 
0121 GTTCGATTCT CAATCGTACC CCTTATGATC ATCAACTTAA TACGCCCCTG AAGTCGGATA 
0181 ATTCCTGTCA GTGCACTATC GAAAAGAAGG CTAGAAAACG CTTTAAGAAA AACAAAGCGG 
0241 TTTCGGTGGC CGAAACCCAG ACAATTAGCG AAGATGTGAC AAGGAATTAT ACTGAAGATA 
0301 TGAAATCTCC AGCTCCAGAG GGATTTCAGA AATTGAAGAA GAGGGTCAAC AGCGGGGAGG 
0361 AATATAAGCC ACAGCGAAAG GTTTCCAAAA CTCCATCAAG AAGTAGCGGA AACTCTACGG 
0421 ATTTTTGTAG ATGTAATCAA CACATTTCAG GACCAACTTA CAAGGACTAT GGTGGTTATA 
0481 GTCCCTCGGA AACGGAAATT  
 Assemble Data 

31817R-4.IR_full       1   GCAGCTCTCGATCGCGAAATAGTACCAGCAATTACCCATCCACGTGGCAACTTAACATCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31817R-4.in silico     1   GCAGCTCTCGATCGCGAAATAGTACCAGCAATTACCCATCCACGTGGCAACTTAACATCA 60


31817R-4.IR_full       61  GCAGAGCTAAAGAGGAACTGAAAAAGGTTTCTAAAGGATCGCTGGACGAGATTATCAATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31817R-4.in silico     61  GCAGAGCTAAAGAGGAACTGAAAAAGGTTTCTAAAGGATCGCTGGACGAGATTATCAATT 120


31817R-4.IR_full       121 GTTCGATTCTCAATCGTACCCCTTATGATCATCAACTTAATACGCCCCTGAAGTCGGATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31817R-4.in silico     121 GTTCGATTCTCAATCGTACCCCTTATGATCATCAACTTAATACGCCCCTGAAGTCGGATA 180


31817R-4.IR_full       181 ATTCCTGTCAGTGCACTATCGACAAGAAGGCTAGAAAACGCTTCAAGAAAAACAAAGCGG 240
                           |||||||||||||||||||||| |||||||||||||||||||| ||||||||||||||||
31817R-4.in silico     181 ATTCCTGTCAGTGCACTATCGAAAAGAAGGCTAGAAAACGCTTTAAGAAAAACAAAGCGG 240


31817R-4.IR_full       241 TTTCGGTGGCCGAAACCCAGACAATCAGCGAAGATGTGACAAGGAATTACACTGAAGATA 300
                           ||||||||||||||||||||||||| ||||||||||||||||||||||| ||||||||||
31817R-4.in silico     241 TTTCGGTGGCCGAAACCCAGACAATTAGCGAAGATGTGACAAGGAATTATACTGAAGATA 300


31817R-4.IR_full       301 TGAAACCTCCAGCTCCAGAGGGATTTCAGAAATTGAAGAAGAGGGTCAACAGCGGGGAGG 360
                           ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
31817R-4.in silico     301 TGAAATCTCCAGCTCCAGAGGGATTTCAGAAATTGAAGAAGAGGGTCAACAGCGGGGAGG 360


31817R-4.IR_full       361 AATATAAGCCACAGCGAAAGGTTTCCAAAACTCCATCAAGAAGTAGCGGAAACTCTACGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31817R-4.in silico     361 AATATAAGCCACAGCGAAAGGTTTCCAAAACTCCATCAAGAAGTAGCGGAAACTCTACGG 420


31817R-4.IR_full       421 ATTTTTGTAGATGTAATCAACACATTTCAGGACCAACTTACAAGGACTATGGGGGTTATA 480
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
31817R-4.in silico     421 ATTTTTGTAGATGTAATCAACACATTTCAGGACCAACTTACAAGGACTATGGTGGTTATA 480


31817R-4.IR_full       481 GTCCCTCGGAAACGGAAATT 500
                           ||||||||||||||||||||
31817R-4.in silico     481 GTCCCTCGGAAACGGAAATT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_165153.1  CG31817-RA (CG31817), mRNA 
0   0  5  3  5  NM_141790.2  CG6666-RA (CG6666), mRNA 
0   0  3  5  4  NM_136456.2  CG1360-RA (CG1360), mRNA 
0   0  2  3  2  NM_166533.1  CG6339-RA (rad50), mRNA 
0   0  1  6  4  NM_058130.4  CG5025-RA, transcript variant A (Sps2), mRNA 
0   0  1  6  4  NM_058129.4  CG5025-RB, transcript variant B (Sps2), mRNA 
0   0  1  3  3  NM_142691.1  CG5798-RA (CG5798), mRNA 
0   0  0  5  9  NM_136864.2  CG13185-RA (CG13185), mRNA 
0   0  0  5  8  NM_134281.1  CG8651-RC, transcript variant C (trx), mRNA 
0   0  0  5  8  NM_057421.2  CG8651-RD, transcript variant D (trx), mRNA 
0   0  0  5  8  NM_057422.2  CG8651-RB, transcript variant B (trx), mRNA 
0   0  0  5  8  NM_134282.1  CG8651-RA, transcript variant A (trx), mRNA 
0   0  0  5  8  NM_001014621.1  CG8651-RE, transcript variant E (trx), mRNA 
0   0  0  4  11  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  0  4  3  NM_140324.2  CG10646-RA (CG10646), mRNA 
0   0  0  4  3  NM_143511.2  CG7946-RA (CG7946), mRNA 
0   0  0  4  2  NM_080271.2  CG4625-RA (Dhap-at), mRNA 
0   0  0  4  2  NM_140247.2  CG6004-RB (CG6004), mRNA 
0   0  0  4  2  NM_168737.1  CG7555-RB, transcript variant B (Nedd4), mRNA 
0   0  0  4  2  NM_206394.1  CG7555-RD, transcript variant D (Nedd4), mRNA 
0   0  0  3  7  NM_142478.2  CG14303-RA (CG14303), mRNA 
0   0  0  3  6  NM_001014681.1  CG33553-RD, transcript variant D (Doa), mRNA 
0   0  0  3  5  NM_143703.2  CG18214-RA, transcript variant A (trio), mRNA 
0   0  0  3  5  NM_167847.1  CG18214-RC, transcript variant C (trio), mRNA 
0   0  0  3  5  NM_057973.3  CG2864-RA (Parg), mRNA 
0   0  0  3  4  NM_001014592.1  CG12306-RB, transcript variant B (polo), mRNA 
0   0  0  3  4  NM_079455.3  CG12306-RA, transcript variant A (polo), mRNA 
0   0  0  3  4  NM_079741.2  CG10210-RA (tst), mRNA 
0   0  0  3  3  NM_170110.2  CG5405-RC, transcript variant C (KrT95D), mRNA 
0   0  0  3  3  NM_001014679.1  CG33553-RC, transcript variant C (Doa), mRNA 

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