NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31769R-2

Symbol CG31769 Full Name CG31769

CG No CG31769 Old CG No CG31769

Synonyms BcDNA:RH26533, CG31769

Accession No (Link to NCBI) NM_165083.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCTAGGCATTGGAACGTGCTTG

Primer Seq. 3'

aatctagaggtaccGTGTGTGGATGTTGGATTTGG

Predicted Fragment Size

IR fragment full Seq

0001 CTAgGCATTG GAACGTGCTT GAAACAGTCG TAAATAATAT GCGGATCACT AAAAGGCCGT 
0061 GGATAGTTAC AAAAACTAAA CCGTGCGGTG AGGTGATTAG GGAAACCTAC ATGTTGAGCC 
0121 CCAACGAAGA GGAACTCATA AGGAGAACGG AAGTCATGCT CTTGCAAGCT CAAGGAAAAT 
0181 TGGAATGTTT ACTTAGCCAG TTGCAATCGA CATTACGACT TGGCAGTGTC TACAATAATC 
0241 CCTTTCTAAA CCCGGTCGGT TTTGGTTTTC CTGACTATAA CGTCCTTCAA AATTTTCCGT 
0301 TTATCCCGGA ATTGAAGCCG CAACGTATTC CTCCCGAAGA AGGTCGCAGT AATGAAAATA 
0361 ATCCTCGCCG AGAAGATCCG CGGGTAAATG AGCAGGATGC GTGGAATTTA CGCCCTCAAG 
0421 ACGTCCCCAA AGANAATGAA CACATTCCNC NATAGTGAAT CGGATAGTGA TAGATGGCTG 
0481 CCAAATCCAA CATCCNCACA C

in silico PCR Fragment

0001 CTAGGCATTG GAACGTGCTT GAAACAGTCG TAAATAATAT GCGGATCACT AAAAGGCCGT 
0061 GGATAGTTAC AAAAACTAAA CCGTGCGGTG AGGTGATTAG GGAAACCTAC ATGTTGAGCC 
0121 CCAACGAAGA GGAACTCATA AGGAGAACGG AAGTCATGCT CTTGCAAGCT CAAGGAAAAT 
0181 TGGAATGTTT ACTTAGCCAG TTTCAACCGA CATTACGACT TGGCAGTGTC TACAATAATC 
0241 CCTTTCTAAA CCCGGTCGGT TTTGGTTTTC CTGACTATAA CGTCTTCCAA AATTTTCCGT 
0301 TTATCCCGGA ATTTAAGCCG CAACGTATTC CTCCCGAAGA AGATCGCAGT AATGAAAATA 
0361 ATCCTCGCCG AGAAGATCCG CGGGTAAATG AGCAGGATGC GTGGAATTTA CGCCCTCAAG 
0421 ACGTCCCCAA AGAAAATGAA CACATTCC-C AATAGTGAAT CGGATAGTGA TAGATGGCTG 
0481 CCAAATCCAA CATCCACACA C

Assemble Data


31769R-2.IR_full       1   CTAGGCATTGGAACGTGCTTGAAACAGTCGTAAATAATATGCGGATCACTAAAAGGCCGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31769R-2.in silico     1   CTAGGCATTGGAACGTGCTTGAAACAGTCGTAAATAATATGCGGATCACTAAAAGGCCGT 60


31769R-2.IR_full       61  GGATAGTTACAAAAACTAAACCGTGCGGTGAGGTGATTAGGGAAACCTACATGTTGAGCC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31769R-2.in silico     61  GGATAGTTACAAAAACTAAACCGTGCGGTGAGGTGATTAGGGAAACCTACATGTTGAGCC 120


31769R-2.IR_full       121 CCAACGAAGAGGAACTCATAAGGAGAACGGAAGTCATGCTCTTGCAAGCTCAAGGAAAAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31769R-2.in silico     121 CCAACGAAGAGGAACTCATAAGGAGAACGGAAGTCATGCTCTTGCAAGCTCAAGGAAAAT 180


31769R-2.IR_full       181 TGGAATGTTTACTTAGCCAGTTGCAATCGACATTACGACTTGGCAGTGTCTACAATAATC 240
                           |||||||||||||||||||||| ||| |||||||||||||||||||||||||||||||||
31769R-2.in silico     181 TGGAATGTTTACTTAGCCAGTTTCAACCGACATTACGACTTGGCAGTGTCTACAATAATC 240


31769R-2.IR_full       241 CCTTTCTAAACCCGGTCGGTTTTGGTTTTCCTGACTATAACGTCCTTCAAAATTTTCCGT 300
                           |||||||||||||||||||||||||||||||||||||||||||| | |||||||||||||
31769R-2.in silico     241 CCTTTCTAAACCCGGTCGGTTTTGGTTTTCCTGACTATAACGTCTTCCAAAATTTTCCGT 300


31769R-2.IR_full       301 TTATCCCGGAATTGAAGCCGCAACGTATTCCTCCCGAAGAAGGTCGCAGTAATGAAAATA 360
                           ||||||||||||| |||||||||||||||||||||||||||| |||||||||||||||||
31769R-2.in silico     301 TTATCCCGGAATTTAAGCCGCAACGTATTCCTCCCGAAGAAGATCGCAGTAATGAAAATA 360


31769R-2.IR_full       361 ATCCTCGCCGAGAAGATCCGCGGGTAAATGAGCAGGATGCGTGGAATTTACGCCCTCAAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31769R-2.in silico     361 ATCCTCGCCGAGAAGATCCGCGGGTAAATGAGCAGGATGCGTGGAATTTACGCCCTCAAG 420


31769R-2.IR_full       421 ACGTCCCCAAAGANAATGAACACATTCCNCNATAGTGAATCGGATAGTGATAGATGGCTG 480
                           ||||||||||||| |||||||||||||| | |||||||||||||||||||||||||||||
31769R-2.in silico     421 ACGTCCCCAAAGAAAATGAACACATTCC-CAATAGTGAATCGGATAGTGATAGATGGCTG 480


31769R-2.IR_full       481 CCAAATCCAACATCCNCACAC 501
                           ||||||||||||||| |||||
31769R-2.in silico     481 CCAAATCCAACATCCACACAC 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_165083.2	CG31769-RA (CG31769), mRNA
0	0	3	2	2	NM_140580.1	CG5414-RA (CG5414), mRNA
0	0	2	3	2	NM_136815.1	CG13222-RA (CG13222), mRNA
0	0	1	3	3	NM_080152.2	CG5092-RA (Tor), mRNA
0	0	1	3	2	NM_079743.2	CG5163-RA (TfIIA-S), mRNA
0	0	0	5	2	NM_167261.1	CG32670-RA (CG32670), mRNA
0	0	0	4	4	NR_001945.1	CR32489, miscRNA
0	0	0	4	3	NM_144487.1	CG5518-RA (sda), mRNA
0	0	0	4	2	NM_167260.1	CG32671-RA (CG32671), mRNA
0	0	0	4	2	NM_132399.1	CG2885-RA (RabX2), mRNA
0	0	0	4	2	NM_132414.1	CG9807-RA (CG9807), mRNA
0	0	0	4	2	NM_167238.1	CG32673-RA (CG32673), mRNA
0	0	0	4	2	NM_167237.1	CG32678-RA (Rab9D), mRNA
0	0	0	3	3	NM_144357.2	CG13475-RA (HGTX), mRNA
0	0	0	3	3	NM_164430.2	CG31666-RD, transcript variant D (CG31666), mRNA
0	0	0	3	3	NM_175956.1	CG33003-RA (CG33003), mRNA
0	0	0	3	2	NM_166128.1	CG18255-RG, transcript variant G (Strn-Mlck), mRNA
0	0	0	3	2	NM_169973.1	CG31173-RA (Gr93c), mRNA
0	0	0	3	2	NM_166125.2	CG18255-RA, transcript variant A (Strn-Mlck), mRNA
0	0	0	3	2	NM_166126.1	CG18255-RC, transcript variant C (Strn-Mlck), mRNA
0	0	0	3	2	NM_138036.2	CG3209-RA, transcript variant A (CG3209), mRNA
0	0	0	3	2	NM_166658.1	CG3209-RB, transcript variant B (CG3209), mRNA
0	0	0	3	2	NM_166127.1	CG18255-RF, transcript variant F (Strn-Mlck), mRNA
0	0	0	3	2	NM_135100.2	CG7251-RA (CG7251), mRNA
0	0	0	3	2	NM_137227.2	CG8315-RA (CG8315), mRNA
0	0	0	3	2	NM_079030.2	CG18255-RB, transcript variant B (Strn-Mlck), mRNA
0	0	0	3	2	NM_137905.2	CG30194-RB, transcript variant B (CG30194), mRNA
0	0	0	3	2	NM_206203.1	CG30194-RC, transcript variant C (CG30194), mRNA
0	0	0	3	2	NM_206204.1	CG30194-RD, transcript variant D (CG30194), mRNA
0	0	0	3	2	NM_165368.1	CG18362-RE, transcript variant E (Mio), mRNA

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