NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 31362R-2

Symbol Jon99Ciii Full Name Jonah 99Ciii

CG No CG31362 Old CG No CG31362

Synonyms Ser99Db, 99Calpha, Jon99Cbeta, Jon99Calpha, Jon99Cbetaiii, Jon99Calphaii, SP143, CG15519, SER2, Ser5, Ser2, Jon99C1, Jon99C2, Jon99C, CG31362, JonC, Jon99B, Jon99Ciii

Accession No (Link to NCBI) NM_170451.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGACGCCCATCAAGGACATTCA

Primer Seq. 3'

aatctagaggtaccAGGTGCGGCTGCAATCACTTT

Predicted Fragment Size

IR fragment full Seq

0001 GACGCCCATC AAGGACATTC AGGGTCGCAT CACCAAACGG CTACCCAGCC TACGAGGGCA 
0061 AGGTGCCTTA CATCGTGGGT CTGCTCTTCA GCGGCAACGG AAACTGGTGG TGCGGTGGCT 
0121 CGATCATCGG CAACACCTGG GTCCTGACCG CCGCTCACTG CACCAACGGA GCCAGTGGAG 
0181 TGACCATCAA CTACGGAGCC AGCATCCGCA CCCAGCCCCA GTACAcCCAC TGGGTGGGCA 
0241 GTGGCGACAT CATCCAGCAC CACCACTACA ACAGCGGCAA CCTGCACAAC GACATCTCCC 
0301 TGATCCGTAC CCCGCACGTC GACTTCTGGA GCCTGGTCAA CAAGGTTGAG CTGCCCAGCT 
0361 ACAACGACCG CTACCAgGAC TACGCCGGAT GGTgGGCCGT GGcCTCCGGA TGGGGCGGCA 
0421 CCTACGATgG CAGCCCACTG CCCGACTGGC TCCAGTCCGT CGATGTCCAG ATCATTTCCC 
0481 AAAGTGATTG CAgNCGCACC T

in silico PCR Fragment

0001 GACGCCCATC AAGGACATTC AGGGTCGCAT CACC-AACGG CTACCCAGCC TACGAGGGCA 
0061 AGGTGCCTTA CATCGTGGGT CTGCTCTTCA GCGGCAACGG AAACTGGTGG TGCGGTGGCT 
0121 CGATCATCGG CAACACCTGG GTCCTGACCG CCGCTCACTG CACCAACGGA GCCAGTGGAG 
0181 TGACCATCAA CTACGGAGCC AGCATCCGCA CCCAGCCCCA GTACACCCAC TGGGTGGGCA 
0241 GTGGCGACAT CATCCAGCAC CACCACTACA ACAGCGGCAA CCTGCACAAC GACATCTCCC 
0301 TGATCCGTAC CCCGCACGTC GACTTCTGGA GCCTGGTCAA CAAGGTTGAG CTGCCCAGCT 
0361 ACAACGACCG CTACCAGGAC TACGCCGGAT GGTGGGCCGT GGCCTCCGGA TGGGGCGGCA 
0421 CCTACGATGG CAGCCCACTG CCCGACTGGC TCCAGTCCGT CGATGTCCAG ATCATTTCCC 
0481 AAAGTGATTG CAGCCGCACC T

Assemble Data


31362R-2.IR_full       1   GACGCCCATCAAGGACATTCAGGGTCGCATCACCAAACGGCTACCCAGCCTACGAGGGCA 60
                           |||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||
31362R-2.in silico     1   GACGCCCATCAAGGACATTCAGGGTCGCATCACC-AACGGCTACCCAGCCTACGAGGGCA 60


31362R-2.IR_full       61  AGGTGCCTTACATCGTGGGTCTGCTCTTCAGCGGCAACGGAAACTGGTGGTGCGGTGGCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     61  AGGTGCCTTACATCGTGGGTCTGCTCTTCAGCGGCAACGGAAACTGGTGGTGCGGTGGCT 120


31362R-2.IR_full       121 CGATCATCGGCAACACCTGGGTCCTGACCGCCGCTCACTGCACCAACGGAGCCAGTGGAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     121 CGATCATCGGCAACACCTGGGTCCTGACCGCCGCTCACTGCACCAACGGAGCCAGTGGAG 180


31362R-2.IR_full       181 TGACCATCAACTACGGAGCCAGCATCCGCACCCAGCCCCAGTACACCCACTGGGTGGGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     181 TGACCATCAACTACGGAGCCAGCATCCGCACCCAGCCCCAGTACACCCACTGGGTGGGCA 240


31362R-2.IR_full       241 GTGGCGACATCATCCAGCACCACCACTACAACAGCGGCAACCTGCACAACGACATCTCCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     241 GTGGCGACATCATCCAGCACCACCACTACAACAGCGGCAACCTGCACAACGACATCTCCC 300


31362R-2.IR_full       301 TGATCCGTACCCCGCACGTCGACTTCTGGAGCCTGGTCAACAAGGTTGAGCTGCCCAGCT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     301 TGATCCGTACCCCGCACGTCGACTTCTGGAGCCTGGTCAACAAGGTTGAGCTGCCCAGCT 360


31362R-2.IR_full       361 ACAACGACCGCTACCAGGACTACGCCGGATGGTGGGCCGTGGCCTCCGGATGGGGCGGCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     361 ACAACGACCGCTACCAGGACTACGCCGGATGGTGGGCCGTGGCCTCCGGATGGGGCGGCA 420


31362R-2.IR_full       421 CCTACGATGGCAGCCCACTGCCCGACTGGCTCCAGTCCGTCGATGTCCAGATCATTTCCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31362R-2.in silico     421 CCTACGATGGCAGCCCACTGCCCGACTGGCTCCAGTCCGTCGATGTCCAGATCATTTCCC 480


31362R-2.IR_full       481 AAAGTGATTGCAGNCGCACCT 501
                           ||||||||||||| |||||||
31362R-2.in silico     481 AAAGTGATTGCAGCCGCACCT 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc