National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock discarded request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31332R-1 
 Symbol unc-115  Full Name unc-115 
 CG No CG31332  Old CG No CG31332 
 Synonyms dunc-115, CG9489, CG31332, anon-WO0140519.9, unc-115 
 Accession No (Link to NCBI) NM_169302.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGTTTTCGTCCCCTTTTCCCC 
 Primer Seq. 3' 
aatctagaggtaccAGATCACACTGCCGTGCTGCT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGTTTTCGTC CCCTTTTCCC CGCAAACAGG TAAACAAAAA ATCTACTGTG CGAAATGCAC 
0061 GAAGAAGTGC TCCGGcGAGG TGCTCCgNGT GgcGGNCAAC CACTTCCACA AGGCCTGCTT 
0121 CCAGTGCTGC CAGTGCAAGA AGTCCCTGGC CACCGGCGGA TTCTTCACAA AGGACAACGC 
0181 CTACTATTGC ATACCGGACT ATCAGCGGCT GTACGGCACC AAGTGCGCAA ATTGCCAGCA 
0241 GTATGTGGAG GGCGAGGTGG TCAGCACCAT GGGCAAGACC TATCACCAGA AGTGCTTCAC 
0301 CTGCTCCAAG TGCAAGCAGC CCTTCAAGTC GGGCAGTAAG GTGACCAACA CCGGAAAGGA 
0361 GGTGCTTTGC GAGCAATGCG TCACGGGTGC TCCAGTGTCG CCCAGtcgCC AGGCGACGGG 
0421 TGGAGGCGTC TCCTCGCCNG CTcNTCCGGC GGAGANTCCG ACGAGATGCC ACTGCCCACC 
0481 AGCAGCACGG CAGTGTGNTC T 
 in silico PCR Fragment 
0001 CGTTTTCGTC CCCTTTTCCC CGCAAACAGG TAAACAAAAA ATCTACTGTG CGAAATGCAC 
0061 GAAGAAGTGC TCCGGCGAGG TGCTCCGCGT GGCGGACAAC CACTTCCACA AGGCCTGCTT 
0121 CCAGTGCTGC CAGTGCAAGA AGTCCCTGGC CACCGGCGGA TTCTTCACAA AGGACAACGC 
0181 CTACTATTGC ATACCGGACT ATCAGCGGCT GTACGGCACC AAGTGCGCAA ATTGCCAGCA 
0241 GTATGTGGAG GGCGAGGTGG TCAGCACCAT GGGCAAGACC TATCACCAGA AGTGCTTCAC 
0301 CTGCTCCAAG TGCAAGCAGC CCTTCAAGTC GGGCAGTAAG GTGACCAACA CCGGAAAGGA 
0361 GGTGCTTTGC GAGCAATGCG TCACGGGTGC TCCAGTGTCG CCCAGTCGCC AGGCGACGGG 
0421 TGGAGGCGTC TCCTCGCCAG CTCCTCCGGC GGAGAGTCCG ACGAGA-GCC ACTGCCCACC 
0481 AGCAGCACGG CAGTGTGATC T 
 Assemble Data 

31332R-1.IR_full       1   CGTTTTCGTCCCCTTTTCCCCGCAAACAGGTAAACAAAAAATCTACTGTGCGAAATGCAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     1   CGTTTTCGTCCCCTTTTCCCCGCAAACAGGTAAACAAAAAATCTACTGTGCGAAATGCAC 60


31332R-1.IR_full       61  GAAGAAGTGCTCCGGCGAGGTGCTCCGNGTGGCGGNCAACCACTTCCACAAGGCCTGCTT 120
                           ||||||||||||||||||||||||||| ||||||| ||||||||||||||||||||||||
31332R-1.in silico     61  GAAGAAGTGCTCCGGCGAGGTGCTCCGCGTGGCGGACAACCACTTCCACAAGGCCTGCTT 120


31332R-1.IR_full       121 CCAGTGCTGCCAGTGCAAGAAGTCCCTGGCCACCGGCGGATTCTTCACAAAGGACAACGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     121 CCAGTGCTGCCAGTGCAAGAAGTCCCTGGCCACCGGCGGATTCTTCACAAAGGACAACGC 180


31332R-1.IR_full       181 CTACTATTGCATACCGGACTATCAGCGGCTGTACGGCACCAAGTGCGCAAATTGCCAGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     181 CTACTATTGCATACCGGACTATCAGCGGCTGTACGGCACCAAGTGCGCAAATTGCCAGCA 240


31332R-1.IR_full       241 GTATGTGGAGGGCGAGGTGGTCAGCACCATGGGCAAGACCTATCACCAGAAGTGCTTCAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     241 GTATGTGGAGGGCGAGGTGGTCAGCACCATGGGCAAGACCTATCACCAGAAGTGCTTCAC 300


31332R-1.IR_full       301 CTGCTCCAAGTGCAAGCAGCCCTTCAAGTCGGGCAGTAAGGTGACCAACACCGGAAAGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     301 CTGCTCCAAGTGCAAGCAGCCCTTCAAGTCGGGCAGTAAGGTGACCAACACCGGAAAGGA 360


31332R-1.IR_full       361 GGTGCTTTGCGAGCAATGCGTCACGGGTGCTCCAGTGTCGCCCAGTCGCCAGGCGACGGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31332R-1.in silico     361 GGTGCTTTGCGAGCAATGCGTCACGGGTGCTCCAGTGTCGCCCAGTCGCCAGGCGACGGG 420


31332R-1.IR_full       421 TGGAGGCGTCTCCTCGCCNGCTCNTCCGGCGGAGANTCCGACGAGATGCCACTGCCCACC 480
                           |||||||||||||||||| |||| ||||||||||| |||||||||| |||||||||||||
31332R-1.in silico     421 TGGAGGCGTCTCCTCGCCAGCTCCTCCGGCGGAGAGTCCGACGAGA-GCCACTGCCCACC 480


31332R-1.IR_full       481 AGCAGCACGGCAGTGTGNTCT 501
                           ||||||||||||||||| |||
31332R-1.in silico     481 AGCAGCACGGCAGTGTGATCT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_169303.2  CG31332-RB, transcript variant B (unc-115), mRNA 
100   482  0  0  1  NM_169302.1  CG31332-RA, transcript variant A (unc-115), mRNA 
94.39   455  1  1  2  NM_169301.2  CG31332-RC, transcript variant C (unc-115), mRNA 
94.19   454  1  2  2  NM_141673.4  CG31352-RA (CG31352), mRNA 
94.19   454  1  1  2  NM_169300.2  CG31332-RD, transcript variant D (unc-115), mRNA 
0.2   1  2  2  5  NM_080712.5  CG1693-RA, transcript variant A (tty), mRNA 
0.2   1  2  2  4  NM_167744.3  CG1693-RB, transcript variant B (tty), mRNA 
0   0  4  11  15  NM_137979.2  CG13560-RA (CG13560), mRNA 
0   0  3  3  12  NM_001043082.1  CG11798-RC, transcript variant C (chn), mRNA 
0   0  3  3  11  NM_137169.3  CG11798-RA, transcript variant A (chn), mRNA 
0   0  3  3  11  NM_206119.1  CG11798-RB, transcript variant B (chn), mRNA 
0   0  3  2  6  NM_001043081.1  CG11798-RD, transcript variant D (chn), mRNA 
0   0  3  2  3  NM_137101.2  CG8531-RA (CG8531), mRNA 
0   0  2  4  4  NM_170454.1  CG11504-RA, transcript variant A (CG11504), mRNA 
0   0  2  4  4  NM_143501.2  CG11504-RB, transcript variant B (CG11504), mRNA 
0   0  2  4  4  NM_176584.1  CG11504-RC, transcript variant C (CG11504), mRNA 
0   0  2  3  8  NM_078999.2  CG3830-RA (vg), mRNA 
0   0  2  3  6  NM_139905.2  CG8111-RA (CG8111), mRNA 
0   0  2  3  3  NM_166664.1  CG4057-RA, transcript variant A (tamo), mRNA 
0   0  2  3  3  NM_138045.2  CG4057-RB, transcript variant B (tamo), mRNA 
0   0  2  2  4  NM_079679.2  CG5557-RA (sqz), mRNA 
0   0  2  2  3  NM_169919.1  CG31203-RB, transcript variant B (CG31203), mRNA 
0   0  2  2  3  NM_169921.1  CG31203-RE, transcript variant E (CG31203), mRNA 
0   0  2  2  3  NM_169918.1  CG31203-RA, transcript variant A (CG31203), mRNA 
0   0  2  2  3  NM_169920.1  CG31203-RD, transcript variant D (CG31203), mRNA 
0   0  1  9  7  NM_168420.1  CG32067-RA, transcript variant A (simj), mRNA 
0   0  1  9  7  NM_140150.2  CG32067-RC, transcript variant C (simj), mRNA 
0   0  1  9  7  NM_168421.1  CG32067-RB, transcript variant B (simj), mRNA 
0   0  1  4  4  NM_079031.1  CG8322-RA, transcript variant A (ATPCL), mRNA 
0   0  1  4  4  NM_166131.1  CG8322-RB, transcript variant B (ATPCL), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
++ 
DM
+ 
VM
 
VB
 
Dcompartment   Penetrance
     

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.