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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
31220R-4 |
| Symbol |
CG31220 |
Full Name |
CG31220 |
| CG No |
CG31220 |
Old CG No |
CG31220 |
| Synonyms |
CG16731, SP6, c-SP6, CG31220 |
| Accession No (Link to NCBI) |
NM_169868.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATAGGCGGCACGGAGCCGAAT |
| Primer Seq. 3' |
aatctagaggtaccGCAGCGTGTTTCAGCACCTTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATAGGCGGCA CGGAGCCGAA TCTCAACGAA TATCCGTGGC TGGCCATGCT TCTATATCGA
0061 AATCGTAGTG CCTTCAACCC GGATCGGGAA CTAGTCCCCA GTTGTGGTGG CTCCCTGATT
0121 AACACTCGCT ATGTCCTGAC CGCAGCCCAC TGTGTAACCG ATACGGTCTT GCAAATCCAA
0181 CGCGTCCGCC TGGGCGAGCA CACAACGTCC CATAACCCGG ACTGCATCAG CCGGGGCGCA
0241 CGAATCGTTT GCGCTCCCAC ACATCTGGAC ATCGACGTGG AGTCGATCAC ATCGCATAAT
0301 GACTACGATC CAGCCAATTA CACCTTTCGG AACGACATCG CCCTGGTGCG ACTCAAAGAG
0361 CCAGTGCGAT ATACGATGGC TTACTATCCG ATTTGCGTTC TGGACTACCC AAGGTCACTG
0421 ATGAAATTCA AGATGTACGT GGCTGGATGG GGAAAAACGG GCATGTTCGA CACTGGGAGC
0481 AAGGTGCTGA AACACGNCTN c |
| in silico PCR Fragment |
0001 ATAGGCGGCA CGGAGCCGAA TCTCAACGAA TATCCGTGGC TGGCCATGCT TCTATATCGA
0061 AATCGTAGTG CCTTCAACCC GGATCGGGAG CTAGTCCCCA GTTGTGGTGG CTCCCTGATT
0121 AACACTCGCT ATGTCCTGAC CGCAGCCCAC TGTGTAACCG ATACGGTCTT GCAAATCCAA
0181 CGCGTCCGCC TGGGCGAGCA CACAACGTCC CATAACCCGG ACTGCATCAG CCGGGGCGCA
0241 CGAATCGTTT GCGCTCCCAC ACATCTGGAC ATCGACGTGG AGTCGATCAC ATCGCATAAT
0301 GACTACGATC CAGCCAATTA CACCTTTCGG AACGACATCG CCCTGGTGCG ACTCAAAGAG
0361 CCAGTGCGAT ATACGATGGC TTACTATCCG ATTTGCGTTC TGGACTACCC AAGGTCACTG
0421 ATGAAATTCA AGATGTACGT GGCTGGATGG GGAAAAACGG GCATGTTCGA CACTGGGAGC
0481 AAGGTGCTGA AACACG-CTG C |
| Assemble Data |
31220R-4.IR_full 1 ATAGGCGGCACGGAGCCGAATCTCAACGAATATCCGTGGCTGGCCATGCTTCTATATCGA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 1 ATAGGCGGCACGGAGCCGAATCTCAACGAATATCCGTGGCTGGCCATGCTTCTATATCGA 60
31220R-4.IR_full 61 AATCGTAGTGCCTTCAACCCGGATCGGGAACTAGTCCCCAGTTGTGGTGGCTCCCTGATT 120
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
31220R-4.in silico 61 AATCGTAGTGCCTTCAACCCGGATCGGGAGCTAGTCCCCAGTTGTGGTGGCTCCCTGATT 120
31220R-4.IR_full 121 AACACTCGCTATGTCCTGACCGCAGCCCACTGTGTAACCGATACGGTCTTGCAAATCCAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 121 AACACTCGCTATGTCCTGACCGCAGCCCACTGTGTAACCGATACGGTCTTGCAAATCCAA 180
31220R-4.IR_full 181 CGCGTCCGCCTGGGCGAGCACACAACGTCCCATAACCCGGACTGCATCAGCCGGGGCGCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 181 CGCGTCCGCCTGGGCGAGCACACAACGTCCCATAACCCGGACTGCATCAGCCGGGGCGCA 240
31220R-4.IR_full 241 CGAATCGTTTGCGCTCCCACACATCTGGACATCGACGTGGAGTCGATCACATCGCATAAT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 241 CGAATCGTTTGCGCTCCCACACATCTGGACATCGACGTGGAGTCGATCACATCGCATAAT 300
31220R-4.IR_full 301 GACTACGATCCAGCCAATTACACCTTTCGGAACGACATCGCCCTGGTGCGACTCAAAGAG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 301 GACTACGATCCAGCCAATTACACCTTTCGGAACGACATCGCCCTGGTGCGACTCAAAGAG 360
31220R-4.IR_full 361 CCAGTGCGATATACGATGGCTTACTATCCGATTTGCGTTCTGGACTACCCAAGGTCACTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 361 CCAGTGCGATATACGATGGCTTACTATCCGATTTGCGTTCTGGACTACCCAAGGTCACTG 420
31220R-4.IR_full 421 ATGAAATTCAAGATGTACGTGGCTGGATGGGGAAAAACGGGCATGTTCGACACTGGGAGC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31220R-4.in silico 421 ATGAAATTCAAGATGTACGTGGCTGGATGGGGAAAAACGGGCATGTTCGACACTGGGAGC 480
31220R-4.IR_full 481 AAGGTGCTGAAACACGNCTNC 501
|||||||||||||||| || |
31220R-4.in silico 481 AAGGTGCTGAAACACG-CTGC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_169868.1 |
CG31220-RA (CG31220), mRNA |
| 1.03 |
5 |
4 |
2 |
2 |
NM_167161.1 |
CG2056-RB, transcript variant B (CG2056), mRNA |
| 1.03 |
5 |
4 |
2 |
2 |
NM_132264.2 |
CG2056-RA, transcript variant A (CG2056), mRNA |
| 0.41 |
2 |
2 |
4 |
9 |
NM_165618.1 |
CG8579-RA (Jon44E), mRNA |
| 0 |
0 |
13 |
7 |
29 |
NM_169869.1 |
CG31219-RA (CG31219), mRNA |
| 0 |
0 |
8 |
10 |
15 |
NM_142910.2 |
CG16710-RA (CG16710), mRNA |
| 0 |
0 |
7 |
2 |
7 |
NM_079614.2 |
CG7996-RA (snk), mRNA |
| 0 |
0 |
5 |
13 |
30 |
NM_142918.1 |
CG10232-RA (CG10232), mRNA |
| 0 |
0 |
4 |
8 |
2 |
NM_142343.2 |
CG17475-RA (CG17475), mRNA |
| 0 |
0 |
4 |
3 |
13 |
NM_132921.1 |
CG9673-RA (CG9673), mRNA |
| 0 |
0 |
2 |
7 |
20 |
NM_169517.1 |
CG8870-RA (CG8870), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_165031.1 |
CG31728-RA (CG31728), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_142342.2 |
CG31265-RA (CG31265), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_079830.2 |
CG31034-RA (Jon99Cii), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_143542.1 |
CG2229-RA (Jon99Fii), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_143543.2 |
CG18030-RA (Jon99Fi), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_133116.1 |
CG14190-RA (CG14190), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_138066.2 |
CG15873-RA (CG15873), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_078851.2 |
CG4192-RA (kek3), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_139759.1 |
CG6592-RA (CG6592), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132876.3 |
CG9214-RA, transcript variant A (Tob), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_206760.1 |
CG9214-RB, transcript variant B (Tob), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166608.1 |
CG30184-RA (CG30184), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_143287.1 |
CG5909-RA (CG5909), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_169915.1 |
CG31205-RA (CG31205), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_001031967.1 |
CG7749-RB, transcript variant B (fat2), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140914.2 |
CG7749-RA, transcript variant A (fat2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135781.2 |
CG5867-RA (CG5867), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_140900.2 |
CG8025-RA (Mtr3), mRNA |
| 0 |
0 |
0 |
15 |
8 |
NM_140450.1 |
CG4613-RA (CG4613), mRNA |
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| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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