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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 31131R-2 
 Symbol CG31131  Full Name CG31131 
 CG No CG31131  Old CG No CG31131 
 Synonyms CG31131 
 Accession No (Link to NCBI) NM_170364.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACTACTGGCTACTTCACTGGG 
 Primer Seq. 3' 
aatctagaggtaccGGCAATTCTGTAGTGCTTGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACTACTGGCT ACTTCACTGG GGAAGTATCT ACTACAACGG AGAAAAGTGC CACTGAGGAA 
0061 TCTACAACTA CGGCGGTGGA TTATTCAACC ACCGAATCTA GTTATGTAAC TTCAACCACG 
0121 GATACTTTAT ATTCCACGAC AACAGCTTGG CCGGAAGTCA CCAGCACAGA TGCTATAGAT 
0181 GCCACTGAAT CAGTAGCCCT ATATTCCACA ACAACTTCGA GTCCAGAAGC TGCAGATTCA 
0241 ACGACAGAAA GCAATTCAGA CAGTACTGAA TATGCTGAAT CAACGTTAAT TTATCCAGAT 
0301 TTATCTACAG GATGGCAAGA AACTACTACA GAAACCATTC AGAGCTCTAC AGGATTGCAG 
0361 GAATCAACAA CAACAGAGTT TATGCCAAGT ACTACGGAAT TTCCGGAATC AACGACTACT 
0421 TTAAGTTCTG AATTTTCAGA GAAAGAACTA ATGAAAactA CCACAGAAAT AGAAATCAAT 
0481 CCAAGCACTA CAGAATTGCC  
 in silico PCR Fragment 
0001 ACTACTGGCT ACTTCACTGG GGAAGTATCT ACTACAACGG AGAAAAGTGC CACTGAGGAA 
0061 TCTACAACTA CGGCGGTGGA TTATTCAACC ACCGAATCTA GTTATGTAAC TTCAACCACG 
0121 GATACTTTAT ATTCCACGAC AACAGCTTGG CCGGAAGTCA CCAGCACAGA TGCTATAGAT 
0181 GCCACTGAAT CAGTAGCCCT ATATTCCACA ACAACTTCGA GTCCAGAAGC TGCAGATTCA 
0241 ACGACAGAAA GCAATTCAGA CAGTACTGAA TATGCTGAAT CAACGTTAAT TTATCCAGAT 
0301 TTATCTACAG GATGGCAAGA AACTACTACA GAAACCATTC AGAGCTCTAC AGGATTGCAG 
0361 GAATCAACAA CAACAGAGTT TATGCCAAGT ACTACGGAAT TTCCGGAATC AACGACTACT 
0421 TTAAGTTCTG AATTTTCAGA GAAAGAACTA ATAAAAACTA CCACAGAAAT AGAAATCAAT 
0481 CCAAGCACTA CAGAATTGCC  
 Assemble Data 

31131R-2.IR_full       1   ACTACTGGCTACTTCACTGGGGAAGTATCTACTACAACGGAGAAAAGTGCCACTGAGGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     1   ACTACTGGCTACTTCACTGGGGAAGTATCTACTACAACGGAGAAAAGTGCCACTGAGGAA 60


31131R-2.IR_full       61  TCTACAACTACGGCGGTGGATTATTCAACCACCGAATCTAGTTATGTAACTTCAACCACG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     61  TCTACAACTACGGCGGTGGATTATTCAACCACCGAATCTAGTTATGTAACTTCAACCACG 120


31131R-2.IR_full       121 GATACTTTATATTCCACGACAACAGCTTGGCCGGAAGTCACCAGCACAGATGCTATAGAT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     121 GATACTTTATATTCCACGACAACAGCTTGGCCGGAAGTCACCAGCACAGATGCTATAGAT 180


31131R-2.IR_full       181 GCCACTGAATCAGTAGCCCTATATTCCACAACAACTTCGAGTCCAGAAGCTGCAGATTCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     181 GCCACTGAATCAGTAGCCCTATATTCCACAACAACTTCGAGTCCAGAAGCTGCAGATTCA 240


31131R-2.IR_full       241 ACGACAGAAAGCAATTCAGACAGTACTGAATATGCTGAATCAACGTTAATTTATCCAGAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     241 ACGACAGAAAGCAATTCAGACAGTACTGAATATGCTGAATCAACGTTAATTTATCCAGAT 300


31131R-2.IR_full       301 TTATCTACAGGATGGCAAGAAACTACTACAGAAACCATTCAGAGCTCTACAGGATTGCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     301 TTATCTACAGGATGGCAAGAAACTACTACAGAAACCATTCAGAGCTCTACAGGATTGCAG 360


31131R-2.IR_full       361 GAATCAACAACAACAGAGTTTATGCCAAGTACTACGGAATTTCCGGAATCAACGACTACT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
31131R-2.in silico     361 GAATCAACAACAACAGAGTTTATGCCAAGTACTACGGAATTTCCGGAATCAACGACTACT 420


31131R-2.IR_full       421 TTAAGTTCTGAATTTTCAGAGAAAGAACTAATGAAAACTACCACAGAAATAGAAATCAAT 480
                           |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||
31131R-2.in silico     421 TTAAGTTCTGAATTTTCAGAGAAAGAACTAATAAAAACTACCACAGAAATAGAAATCAAT 480


31131R-2.IR_full       481 CCAAGCACTACAGAATTGCC 500
                           ||||||||||||||||||||
31131R-2.in silico     481 CCAAGCACTACAGAATTGCC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  3  11  32  NM_170364.1  CG31131-RA (CG31131), mRNA 
100   482  3  11  32  NM_170362.1  CG31253-RA (CG31253), mRNA 
0   0  2  3  6  NM_142876.3  CG4467-RA, transcript variant A (CG4467), mRNA 
0   0  2  3  6  NM_001043282.1  CG4467-RB, transcript variant B (CG4467), mRNA 
0   0  2  2  5  NM_206304.1  CG32042-RE, transcript variant E (PGRP-LA), mRNA 
0   0  2  2  5  NM_206305.1  CG32042-RD, transcript variant D (PGRP-LA), mRNA 
0   0  1  3  5  NM_137832.1  CG4554-RA (CG4554), mRNA 
0   0  1  2  5  NM_166453.1  CG30282-RA (CG30282), mRNA 
0   0  1  2  3  NM_078845.3  CG15274-RA (GABA-B-R1), mRNA 
0   0  1  2  2  NM_079517.2  CG11421-RA (Pbprp3), mRNA 
0   0  1  2  2  NM_132869.2  CG9172-RA, transcript variant A (CG9172), mRNA 
0   0  0  17  88  NM_001032052.1  CG33715-RE, transcript variant E (Msp-300), mRNA 
0   0  0  5  3  NM_206391.1  CG33256-RA (CG33256), mRNA 
0   0  0  5  3  NM_168836.1  CG17233-RA, transcript variant A (CG17233), mRNA 
0   0  0  5  3  NM_168837.1  CG17233-RC, transcript variant C (CG17233), mRNA 
0   0  0  5  3  NM_140939.2  CG17233-RB, transcript variant B (CG17233), mRNA 
0   0  0  4  71  NM_206342.1  CG33265-RA (CG33265), mRNA 
0   0  0  4  8  NM_078509.2  CG3929-RA (dx), mRNA 
0   0  0  4  5  NM_080152.2  CG5092-RA (Tor), mRNA 
0   0  0  4  5  NM_078692.2  CG11937-RA (amn), mRNA 
0   0  0  4  4  NM_134820.2  CG10882-RA (CG10882), mRNA 
0   0  0  4  2  NM_176542.1  CG7029-RA (CG7029), mRNA 
0   0  0  3  16  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  3  6  NM_168509.1  CG32109-RA (CG32109), mRNA 
0   0  0  3  5  NM_136621.2  CG8046-RB, transcript variant B (CG8046), mRNA 
0   0  0  3  5  NM_165657.1  CG8046-RA, transcript variant A (CG8046), mRNA 
0   0  0  3  5  NM_080029.3  CG13761-RB (Bzd), mRNA 
0   0  0  3  5  NM_001038739.1  CG9900-RC, transcript variant C (mit(1)15), mRNA 
0   0  0  3  5  NM_080162.3  CG9900-RB, transcript variant B (mit(1)15), mRNA 
0   0  0  3  5  NM_136689.1  CG30007-RB, transcript variant B (CG30007), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Caspase-3 signal
N
 
DB
 
DC
 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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