NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 30393R-1

Symbol CG30393 Full Name CG30393

CG No CG30393 Old CG No CG30393

Synonyms CG15662, CG30393

Accession No (Link to NCBI) NM_166433.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGCTGCAGCAATTGAAACCC

Primer Seq. 3'

aatctagaggtaccTCCACCAAGTCGATGAAGAAC

Predicted Fragment Size

IR fragment full Seq

0001 CAGCTGCAGC AATTGAAACC CCAACAGAGC GTCATGTCGC ACGTCACGGT CACCGAGAGT 
0061 GATGCCGAGT TTGCGATCCT GAATCTGGAT TGCAACGAAG GTTTGGAGAG CTCGGTGACC 
0121 AAGATTCTGG TGGCCATCGA AGAGATGTTC TGCAGTTACT TTGAGGATCT CAAGAAGGAG 
0181 CTGGAACTGC AGAAGATGGC GGCACTGACC TTGAATCAGC TGACCCAACG TTACAGGATC 
0241 AACACGAAGG TGGACACGAG CTGCACGTGT CCGCAGATCT ATGAGATCGA ATCGGATGAC 
0301 TGCGATCATT AACAAATACT ACATCTACTA CCAGGTTATA GCCTGTTCCA ACAATAACCA 
0361 GGGTTGGGTC ATCCACAACA TGCGCCCCTA TGTTCTCCTC TTTCGTCGAG AGTGCGCCCA 
0421 AGTTGAATCT GAACGAGGAT AGTCCCTTCA TCATGGGTGA CGCTTTCCAC AAGCCAATCT 
0481 AAGTTCTTCA TCGACTTGGT GGA

in silico PCR Fragment

0001 CAGCTGCAGC AATTGAAACC CCAACAGAGC GTCATGTCGC ACGTCACGGT CACCGAGAGT 
0061 GATGCCGAGT TTGCGATCTT GAATCTGGAT TGCAACGAAG GTTTGGAGAG CTCGGTGACC 
0121 AAGATTCTGG TGGCCATCGA AGAGATGTTC TGCAGTTACT TTGAGGATCT CAAGAAGGAG 
0181 CTGGAACTGC AGAAGATGGC GGCACTGACC TTGAATCAGC TGACCCAACG TTACAGGATC 
0241 AACACGAAGG TGGACACGAG CTGCACGTGT CCGCAGATCT ATGAGATCGA ATCGGATGA- 
0301 TGCGATCATT AACAAATACT ACATCTACTA CCAGGTGATA GCCTGTTCCA ACAATAACCA 
0361 GGGTTGGGTC ATCCACAACA TGCGCCCCTA TGTTCTCCTC TTTCGTCGAG AGTGCG-CCA 
0421 AGTTGAATCT GAACGAGGAT AGTCCCTTCA TCATGGGTGA CGCTTTCCAC AAGCCAATC- 
0481 AAGTTCTTCA TCGACTTGGT GGA

Assemble Data


30393R-1.IR_full       1   CAGCTGCAGCAATTGAAACCCCAACAGAGCGTCATGTCGCACGTCACGGTCACCGAGAGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30393R-1.in silico     1   CAGCTGCAGCAATTGAAACCCCAACAGAGCGTCATGTCGCACGTCACGGTCACCGAGAGT 60


30393R-1.IR_full       61  GATGCCGAGTTTGCGATCCTGAATCTGGATTGCAACGAAGGTTTGGAGAGCTCGGTGACC 120
                           |||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
30393R-1.in silico     61  GATGCCGAGTTTGCGATCTTGAATCTGGATTGCAACGAAGGTTTGGAGAGCTCGGTGACC 120


30393R-1.IR_full       121 AAGATTCTGGTGGCCATCGAAGAGATGTTCTGCAGTTACTTTGAGGATCTCAAGAAGGAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30393R-1.in silico     121 AAGATTCTGGTGGCCATCGAAGAGATGTTCTGCAGTTACTTTGAGGATCTCAAGAAGGAG 180


30393R-1.IR_full       181 CTGGAACTGCAGAAGATGGCGGCACTGACCTTGAATCAGCTGACCCAACGTTACAGGATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30393R-1.in silico     181 CTGGAACTGCAGAAGATGGCGGCACTGACCTTGAATCAGCTGACCCAACGTTACAGGATC 240


30393R-1.IR_full       241 AACACGAAGGTGGACACGAGCTGCACGTGTCCGCAGATCTATGAGATCGAATCGGATGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
30393R-1.in silico     241 AACACGAAGGTGGACACGAGCTGCACGTGTCCGCAGATCTATGAGATCGAATCGGATGA- 300


30393R-1.IR_full       301 TGCGATCATTAACAAATACTACATCTACTACCAGGTTATAGCCTGTTCCAACAATAACCA 360
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
30393R-1.in silico     301 TGCGATCATTAACAAATACTACATCTACTACCAGGTGATAGCCTGTTCCAACAATAACCA 360


30393R-1.IR_full       361 GGGTTGGGTCATCCACAACATGCGCCCCTATGTTCTCCTCTTTCGTCGAGAGTGCGCCCA 420
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
30393R-1.in silico     361 GGGTTGGGTCATCCACAACATGCGCCCCTATGTTCTCCTCTTTCGTCGAGAGTGCG-CCA 420


30393R-1.IR_full       421 AGTTGAATCTGAACGAGGATAGTCCCTTCATCATGGGTGACGCTTTCCACAAGCCAATCT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| 
30393R-1.in silico     421 AGTTGAATCTGAACGAGGATAGTCCCTTCATCATGGGTGACGCTTTCCACAAGCCAATC- 480


30393R-1.IR_full       481 AAGTTCTTCATCGACTTGGTGGA 503
                           |||||||||||||||||||||||
30393R-1.in silico     481 AAGTTCTTCATCGACTTGGTGGA 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	3	NM_166433.1	CG30393-RA (CG30393), mRNA
1.03	5	32	32	55	NM_137722.3	CG30391-RA (CG30391), mRNA
0	0	5	3	2	NM_133031.1	CG6847-RA (CG6847), mRNA
0	0	3	2	2	NM_057627.3	CG8996-RB, transcript variant B (wal), mRNA
0	0	3	2	2	NM_165847.1	CG8996-RA, transcript variant A (wal), mRNA
0	0	2	4	4	NM_132543.1	CG10362-RA (CG10362), mRNA
0	0	2	4	2	NM_078908.2	CG12845-RA (Tsp42Ef), mRNA
0	0	2	4	2	NM_137427.2	CG5036-RA (CG5036), mRNA
0	0	2	3	3	NM_080327.2	CG3665-RA, transcript variant A (Fas2), mRNA
0	0	2	3	3	NM_167004.1	CG3665-RB, transcript variant B (Fas2), mRNA
0	0	2	3	3	NM_167005.1	CG3665-RC, transcript variant C (Fas2), mRNA
0	0	2	2	3	NM_167618.1	CG32551-RA (CG32551), mRNA
0	0	2	2	2	NM_140048.2	CG4080-RA (CG4080), mRNA
0	0	1	5	3	NM_137718.2	CG9480-RA, transcript variant A (Glycogenin), mRNA
0	0	1	5	2	NM_057433.2	CG4380-RA (usp), mRNA
0	0	1	4	7	NM_167485.1	CG9216-RC, transcript variant C (CG9216), mRNA
0	0	1	4	7	NM_132878.1	CG9216-RA, transcript variant A (CG9216), mRNA
0	0	1	4	5	NM_165064.1	CG7885-RB, transcript variant B (RpII33), mRNA
0	0	1	4	5	NM_058071.3	CG7885-RA, transcript variant A (RpII33), mRNA
0	0	1	3	3	NM_134844.2	CG9964-RA (Cyp309a1), mRNA
0	0	1	3	2	NM_168109.1	CG32232-RA (CG32232), mRNA
0	0	1	2	5	NM_078815.2	CG6098-RA (Lrr47), mRNA
0	0	1	2	5	NM_167437.1	CG6170-RC, transcript variant C (HDAC6), mRNA
0	0	1	2	4	NM_079241.2	CG8114-RB, transcript variant B (pbl), mRNA
0	0	1	2	4	NM_168241.1	CG8114-RA, transcript variant A (pbl), mRNA
0	0	1	2	4	NM_079868.2	CG11335-RA (lox), mRNA
0	0	1	2	3	NM_079892.2	CG11027-RA (Arf102F), mRNA
0	0	1	2	3	NM_130566.2	CG14781-RA (CG14781), mRNA
0	0	1	2	3	NM_137243.2	CG8431-RA (Aats-cys), mRNA
0	0	1	2	1	NM_170579.2	CG2098-RC, transcript variant C (ferrochelatase), mRNA

Back