NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 30382R-1

Symbol CG30382 Full Name CG30382

CG No CG30382 Old CG No CG30382

Synonyms CG30382

Accession No (Link to NCBI)

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63 [ PubMed ID = 22417167 ] [ RRC reference ]

Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGAGTACGCCTTCAAGGCTAT

Primer Seq. 3'

aatctagaggtaccCTTTCTCCTCCGACAAGTTGG

Predicted Fragment Size

IR fragment full Seq

in silico PCR Fragment

Assemble Data


30382R-1.IR full       1   GGAGTACGCCTTCAAGGCTATTGCCCAGGAGAACATCACCACGGTGGCGCTGAAGAGTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     1   GGAGTACGCCTTCAAGGCTATTGCCCAGGAGAACATCACCACGGTGGCGCTGAAGAGTGG 60


30382R-1.IR full       61  CGACTGTGCCGTGGTGGCCACCCAAAAAAAAGTGACCGAGAAGAACATCGTGCCTGAAAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     61  CGACTGTGCCGTGGTGGCCACCCAAAAAAAAGTGACCGAGAAGAACATCGTGCCTGAAAC 120


30382R-1.IR full       121 GGTGACCCACCTGTTCCGCATCACCAAGGACATTGGATGCGCAATGACCGGACGCATAGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     121 GGTGACCCACCTGTTCCGCATCACCAAGGACATTGGATGCGCAATGACCGGACGCATAGC 180


30382R-1.IR full       181 TGATTCCCGCTCACAGGTGCAGAAGGCCAGATACGAGGCCGCCAACTTCCGCTACAAGTA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     181 TGATTCCCGCTCACAGGTGCAGAAGGCCAGATACGAGGCCGCCAACTTCCGCTACAAGTA 240


30382R-1.IR full       241 CGGCTACGAAATGCCCGTGGATGTGCTGTGTCGTCGAATTGCGGATATCAACCAGGTGTA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     241 CGGCTACGAAATGCCCGTGGATGTGCTGTGTCGTCGAATTGCGGATATCAACCAGGTGTA 300


30382R-1.IR full       301 CACGCAGAATGCCGAGATGCGTCCGCTCGGCTGCAGCATGGTGCTGATCGCTTACGACAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     301 CACGCAGAATGCCGAGATGCGTCCGCTCGGCTGCAGCATGGTGCTGATCGCTTACGACAA 360


30382R-1.IR full       361 TGAAATCGGACCCAGCGTTTACAAGACGGACCCGGCTGGCTACTTCAGCGGGTTCAAGGC 420
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     361 TGAAATCGGCCCCAGCGTTTACAAGACGGACCCGGCTGGCTACTTCAGCGGGTTCAAGGC 420


30382R-1.IR full       421 CTGCAGCGTGGGTGCCAAGACTCTGGAGGCCAACAGCTATCTGGAAAAGAAGTACAAGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30382R-1.in silico     421 CTGCAGCGTGGGTGCCAAGACTCTGGAGGCCAACAGCTATCTGGAAAAGAAGTACAAGCC 480


30382R-1.IR full       481 CAACTTGTCGGAGGAGAAAG 500
                           ||||||||||||||||||||
30382R-1.in silico     481 CAACTTGTCGGAGGAGAAAG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

Wing Disc