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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 30289R-6 
 Symbol CG30289  Full Name CG30289 
 CG No CG30289  Old CG No CG30289 
 Synonyms CG30289 
 Accession No (Link to NCBI) NM_166459.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCCTGCTGGTTGAGAATTGTG 
 Primer Seq. 3' 
aatctagaggtaccCAGTAGGATCCGACTGAATTG 
 Predicted Fragment Size 
485 
 IR fragment full Seq 
0001 GCCTGCTGGT TGAGAATTGT GGAATATCAA AGGACGATCC ATACGTTCCG AACATTATTG 
0061 GAGGAGCCAA AACAAATATT CAAGAAAATC CTTGGATGGT GTTGGTTTGG TCGAGTAAGC 
0121 CCTGCGGCGG CTCTCTTATT GCACGGCAAT TCGTCCTGAC ANGCANGCGC ACTGCGTATC 
0181 CTTTGAAGAT CTGTATGTGC GCTTGGGTGA TTACGAAACA CTAGATCCCA TGCCATACTG 
0241 CCTTAACAAT CACTGTATAC CAAAGTTCTA CAACATTAGT GTCGATATGA AAATTGTTCA 
0301 CGAAAATTAT AGTGGCACCA CTCTTCAGAA TGATATAGCA TTGCTTCGTA TGTCCGAAGC 
0361 TGTGGAGTAC TCAGATTATG TTAGGCCAAT TTGCCTTCTT GTCGGCGAGC AAATGCAAAG 
0421 CACTCCAATG TTTACGGTTA CCGGCTGGGG TGAAACAGAA TATGGTCAAT TCAGTCGGAT 
0481 CCTACTG 
 in silico PCR Fragment 
0001 GCCTGCTGGT TGAGAATTGT GGAATATCAA AGGACGATCC ATACGTTCCG AACATTTTTG 
0061 GAGGAGCCAA AACAAATATT CAAGAAAATC CTTGGATGGT GTTGGTTTGG TCGAGTAAGC 
0121 CCTGCGGCGG CTCTCTTATT GCACGGCAAT TCGTCCTGAC A-GCA-GCGC ACTGCGTATC 
0181 CTTTGAAGAT CTGTATGTGC GCTTGGGTGA TTACGAAACA CTAGATCCCA TGCCATACTG 
0241 CCTTAACAAT CACTGTATAC CAAAGTTCTA CAACATTAGT GTGGATATGA AAATTGTTCA 
0301 CGAAAATTAT AATGGCATCA CTCTCCAGAA TGATATAGCA TTGCTTCGTA TGTCCGAAGC 
0361 TGTGGAGTAC TCAGATTATG TTAGGCCAAT TTGCCTTCTT GTCGGCGAGC AAATGCAAAG 
0421 CATTCCAATG TTTACGGTTA CCGGCTGGGG TGAAACAGAA TATGGTCAAT TCAGTCGGAT 
0481 CCTACTG 
 Assemble Data 

30289R-6.IR_full       1   GCCTGCTGGTTGAGAATTGTGGAATATCAAAGGACGATCCATACGTTCCGAACATTATTG 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
30289R-6.in silico     1   GCCTGCTGGTTGAGAATTGTGGAATATCAAAGGACGATCCATACGTTCCGAACATTTTTG 60


30289R-6.IR_full       61  GAGGAGCCAAAACAAATATTCAAGAAAATCCTTGGATGGTGTTGGTTTGGTCGAGTAAGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30289R-6.in silico     61  GAGGAGCCAAAACAAATATTCAAGAAAATCCTTGGATGGTGTTGGTTTGGTCGAGTAAGC 120


30289R-6.IR_full       121 CCTGCGGCGGCTCTCTTATTGCACGGCAATTCGTCCTGACANGCANGCGCACTGCGTATC 180
                           ||||||||||||||||||||||||||||||||||||||||| ||| ||||||||||||||
30289R-6.in silico     121 CCTGCGGCGGCTCTCTTATTGCACGGCAATTCGTCCTGACA-GCA-GCGCACTGCGTATC 180


30289R-6.IR_full       181 CTTTGAAGATCTGTATGTGCGCTTGGGTGATTACGAAACACTAGATCCCATGCCATACTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30289R-6.in silico     181 CTTTGAAGATCTGTATGTGCGCTTGGGTGATTACGAAACACTAGATCCCATGCCATACTG 240


30289R-6.IR_full       241 CCTTAACAATCACTGTATACCAAAGTTCTACAACATTAGTGTCGATATGAAAATTGTTCA 300
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
30289R-6.in silico     241 CCTTAACAATCACTGTATACCAAAGTTCTACAACATTAGTGTGGATATGAAAATTGTTCA 300


30289R-6.IR_full       301 CGAAAATTATAGTGGCACCACTCTTCAGAATGATATAGCATTGCTTCGTATGTCCGAAGC 360
                           ||||||||||| ||||| |||||| |||||||||||||||||||||||||||||||||||
30289R-6.in silico     301 CGAAAATTATAATGGCATCACTCTCCAGAATGATATAGCATTGCTTCGTATGTCCGAAGC 360


30289R-6.IR_full       361 TGTGGAGTACTCAGATTATGTTAGGCCAATTTGCCTTCTTGTCGGCGAGCAAATGCAAAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30289R-6.in silico     361 TGTGGAGTACTCAGATTATGTTAGGCCAATTTGCCTTCTTGTCGGCGAGCAAATGCAAAG 420


30289R-6.IR_full       421 CACTCCAATGTTTACGGTTACCGGCTGGGGTGAAACAGAATATGGTCAATTCAGTCGGAT 480
                           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
30289R-6.in silico     421 CATTCCAATGTTTACGGTTACCGGCTGGGGTGAAACAGAATATGGTCAATTCAGTCGGAT 480


30289R-6.IR_full       481 CCTACTG 487
                           |||||||
30289R-6.in silico     481 CCTACTG 487



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   467  0  1  1  NM_166459.1  CG30289-RA (CG30289), mRNA 
0   0  6  3  2  NM_169517.1  CG8870-RA (CG8870), mRNA 
0   0  4  4  2  NM_169258.1  CG9755-RA, transcript variant A (pum), mRNA 
0   0  4  4  2  NM_169259.1  CG9755-RD, transcript variant D (pum), mRNA 
0   0  4  4  2  NM_079561.2  CG9755-RC, transcript variant C (pum), mRNA 
0   0  2  2  3  NM_165262.2  CG31753-RA (ham), mRNA 
0   0  2  2  3  NM_166461.1  CG30287-RA (CG30287), mRNA 
0   0  2  2  2  NM_142106.1  CG14843-RA (CG14843), mRNA 
0   0  2  2  2  NM_166458.1  CG30288-RA (CG30288), mRNA 
0   0  1  5  3  NM_141016.1  CG10587-RA (CG10587), mRNA 
0   0  1  4  4  NM_142094.1  CG14839-RA (CG14839), mRNA 
0   0  1  2  3  NM_078967.2  CG12387-RA (zetaTry), mRNA 
0   0  1  2  2  NM_137665.1  CG30152-RA (CG30152), mRNA 
0   0  1  2  2  NM_079204.1  CG7525-RA (Tie), mRNA 
0   0  0  7  5  NM_142343.2  CG17475-RA (CG17475), mRNA 
0   0  0  5  6  NM_001038880.1  CG3204-RB, transcript variant B (Rap2l), mRNA 
0   0  0  5  6  NM_057836.3  CG3730-RA, transcript variant A (csul), mRNA 
0   0  0  5  6  NM_166158.1  CG3730-RB, transcript variant B (csul), mRNA 
0   0  0  5  5  NM_169748.1  CG31269-RA (CG31269), mRNA 
0   0  0  5  4  NM_130564.2  CG14780-RA (CG14780), mRNA 
0   0  0  5  2  NM_166886.1  CG32808-RA (CG32808), mRNA 
0   0  0  4  9  NM_001031867.1  CG33950-RF, transcript variant F (trol), mRNA 
0   0  0  4  9  NM_001031863.1  CG33950-RD, transcript variant D (trol), mRNA 
0   0  0  4  8  NM_001031862.1  CG33950-RC, transcript variant C (trol), mRNA 
0   0  0  4  8  NM_001031864.1  CG33950-RE, transcript variant E (trol), mRNA 
0   0  0  4  8  NM_001031866.1  CG33950-RA, transcript variant A (trol), mRNA 
0   0  0  4  7  NM_057398.3  CG8049-RA, transcript variant A (Btk29A), mRNA 
0   0  0  4  7  NM_164805.1  CG8049-RC, transcript variant C (Btk29A), mRNA 
0   0  0  4  7  NM_164804.1  CG8049-RD, transcript variant D (Btk29A), mRNA 
0   0  0  4  7  NM_057397.3  CG8049-RB, transcript variant B (Btk29A), mRNA 

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