NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 17760R-1

Symbol CG17760 Full Name CG17760

CG No CG17760 Old CG No CG17760

Synonyms BcDNA:SD21019, CG17760

Accession No (Link to NCBI) NM_001043074.1

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTTGGACAAGGAGAGGAAGCA

Primer Seq. 3'

aatctagaggtaccCGGACATGGCAACCAAAAACA

Predicted Fragment Size

IR fragment full Seq

0001 TTTGGACAAG GAGAGGAAGC AGTTCACCAA AAATGTTTTC CAAAACATAT TCATGGCCAT 
0061 GCAGTCGATG ATCAGTGCAA TGGATACTCT GCGAATTCCC TACGGTCAGC AGGAGCATAG 
0121 TAAACTTGCC GATCTGGTGA AGAGCATCGA TTACAAGACA GTTACCAGAC TGGAGGCGCC 
0181 CTACTTGAAT GCCATCAAAA CGCTTTGGAA AGATGCTGGC ATCAAGGAGT GCTACAATCG 
0241 TCGTAGGGAA TATCAGCTGA CTGATTCAAC TGAATACTTT TTGAATGACA TAGCGCGAAT 
0301 TGAACGGAGT GATTACCAGG CCACTGATCA GGACATTCTT CATGTTCGAG CGCCAACAAC 
0361 CAATATTGTT GAGTATCCCT TCAATTTGGA CGGTTTTCTG ATAAGATTGG TGGACGTCGC 
0421 TGGTCAGCGA ACGGAGAGAA GAAAGTGGAT TCATTGTTTT TCCAATGTGA CATCAATTAT 
0481 GTTTTTGGTT GCCATGTCCG

in silico PCR Fragment

0001 TTTGGACAAG GAGAGGAAGC AGTTCACCAA AAATGTTTTC CAAAACATAT TCATGGCCAT 
0061 ACAGTCGATG ATCAGTGCAA TGGATACTCT GCGAATTCCC TACGGTCAGC AGGAGCATAG 
0121 TAAACTTGCC GATCTGGTGA AGAGCATCGA TTACAAGACA GTTACCAGAC TGGAGGCGCC 
0181 CTACTTGAAT GCCATCAAAA CTCTTTGGAA AGATGCTGGC ATCAAGGAGT GCTACAATCG 
0241 TCGTAGGGAA TATCAGCTGA CTGATTCAAC TGAATACTTT TTGAATGACA TAGCGCGAAT 
0301 TGAACGGAGT GATTACCAGG CCACTGATCA GGACATTCTT CATGTTCGAG CGCCAACAAC 
0361 CAATATTGTT GAGTATCCCT TCAATTTGGA CGGTTTTCTG ATAAGATTGG TGGACGTCGC 
0421 TGGTCAGCGA ACGGAGAGAA GAAAGTGGAT TCATTGTTTT TCCAATGTGA CATCAATTAT 
0481 GTTTTTGGTT GCCATGTCCG

Assemble Data


17760R-1.IR_full       1   TTTGGACAAGGAGAGGAAGCAGTTCACCAAAAATGTTTTCCAAAACATATTCATGGCCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     1   TTTGGACAAGGAGAGGAAGCAGTTCACCAAAAATGTTTTCCAAAACATATTCATGGCCAT 60


17760R-1.IR_full       61  GCAGTCGATGATCAGTGCAATGGATACTCTGCGAATTCCCTACGGTCAGCAGGAGCATAG 120
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     61  ACAGTCGATGATCAGTGCAATGGATACTCTGCGAATTCCCTACGGTCAGCAGGAGCATAG 120


17760R-1.IR_full       121 TAAACTTGCCGATCTGGTGAAGAGCATCGATTACAAGACAGTTACCAGACTGGAGGCGCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     121 TAAACTTGCCGATCTGGTGAAGAGCATCGATTACAAGACAGTTACCAGACTGGAGGCGCC 180


17760R-1.IR_full       181 CTACTTGAATGCCATCAAAACGCTTTGGAAAGATGCTGGCATCAAGGAGTGCTACAATCG 240
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     181 CTACTTGAATGCCATCAAAACTCTTTGGAAAGATGCTGGCATCAAGGAGTGCTACAATCG 240


17760R-1.IR_full       241 TCGTAGGGAATATCAGCTGACTGATTCAACTGAATACTTTTTGAATGACATAGCGCGAAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     241 TCGTAGGGAATATCAGCTGACTGATTCAACTGAATACTTTTTGAATGACATAGCGCGAAT 300


17760R-1.IR_full       301 TGAACGGAGTGATTACCAGGCCACTGATCAGGACATTCTTCATGTTCGAGCGCCAACAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     301 TGAACGGAGTGATTACCAGGCCACTGATCAGGACATTCTTCATGTTCGAGCGCCAACAAC 360


17760R-1.IR_full       361 CAATATTGTTGAGTATCCCTTCAATTTGGACGGTTTTCTGATAAGATTGGTGGACGTCGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     361 CAATATTGTTGAGTATCCCTTCAATTTGGACGGTTTTCTGATAAGATTGGTGGACGTCGC 420


17760R-1.IR_full       421 TGGTCAGCGAACGGAGAGAAGAAAGTGGATTCATTGTTTTTCCAATGTGACATCAATTAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17760R-1.in silico     421 TGGTCAGCGAACGGAGAGAAGAAAGTGGATTCATTGTTTTTCCAATGTGACATCAATTAT 480


17760R-1.IR_full       481 GTTTTTGGTTGCCATGTCCG 500
                           ||||||||||||||||||||
17760R-1.in silico     481 GTTTTTGGTTGCCATGTCCG 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	2	NM_001043074.1	CG17760-RB (CG17760), mRNA
4.35	21	47	80	78	NM_165925.1	CG17759-RF, transcript variant F (Galpha49B), mRNA
4.35	21	47	80	78	NM_165924.1	CG17759-RD, transcript variant D (Galpha49B), mRNA
4.35	21	47	77	78	NM_165919.1	CG17759-RA, transcript variant A (Galpha49B), mRNA
4.35	21	47	77	78	NM_165923.1	CG17759-RG, transcript variant G (Galpha49B), mRNA
4.35	21	47	77	69	NM_165921.1	CG17759-RC, transcript variant C (Galpha49B), mRNA
4.35	21	47	77	69	NM_165922.1	CG17759-RE, transcript variant E (Galpha49B), mRNA
4.35	21	47	77	69	NM_078994.2	CG17759-RH, transcript variant H (Galpha49B), mRNA
4.35	21	47	77	69	NM_165920.1	CG17759-RB, transcript variant B (Galpha49B), mRNA
3.11	15	45	87	92	NM_001043073.1	CG30054-RC (CG30054), mRNA
0	0	4	3	3	NM_135265.1	CG4971-RA (Wnt10), mRNA
0	0	2	16	16	NM_165363.1	CG31626-RB, transcript variant B (CG31626), mRNA
0	0	2	16	16	NM_165362.1	CG31626-RA, transcript variant A (CG31626), mRNA
0	0	2	16	16	NM_136257.2	CG8677-RA (CG8677), mRNA
0	0	2	3	3	NM_166260.1	CG30110-RA (CG30110), mRNA
0	0	2	3	2	NM_139920.1	CG7504-RA (CG7504), mRNA
0	0	2	2	3	NM_057576.3	CG6407-RA (Wnt5), mRNA
0	0	2	2	2	NM_136745.3	CG12892-RA (Caf1-105), mRNA
0	0	1	3	3	NM_137456.2	CG5733-RA (CG5733), mRNA
0	0	1	3	2	NM_205982.1	CG6214-RM, transcript variant M (MRP), mRNA
0	0	1	3	2	NM_205980.1	CG6214-RO, transcript variant O (MRP), mRNA
0	0	1	3	2	NM_205976.1	CG6214-RD, transcript variant D (MRP), mRNA
0	0	1	3	2	NM_205975.1	CG6214-RE, transcript variant E (MRP), mRNA
0	0	1	3	2	NM_205974.1	CG6214-RF, transcript variant F (MRP), mRNA
0	0	1	3	2	NM_205977.1	CG6214-RC, transcript variant C (MRP), mRNA
0	0	1	3	2	NM_165020.2	CG6214-RA, transcript variant A (MRP), mRNA
0	0	1	3	2	NM_205973.1	CG6214-RG, transcript variant G (MRP), mRNA
0	0	1	3	2	NM_205979.1	CG6214-RP, transcript variant P (MRP), mRNA
0	0	1	3	2	NM_205972.1	CG6214-RH, transcript variant H (MRP), mRNA
0	0	1	3	2	NM_205970.1	CG6214-RJ, transcript variant J (MRP), mRNA

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