NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 1773R-1

Symbol CG1773 Full Name CG1773

CG No CG1773 Old CG No CG1773

Synonyms SP74, CG1773

Accession No (Link to NCBI) NM_136668.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Fan W, Lam SM, Xin J, Yang X, Liu Z, Liu Y, Wang Y, Shui G, Huang X.
Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.
PLoS Genet (2017) 13(3) e1006664 [ PubMed ID = 28273089 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGAACAGTCCTCTCGGGATC

Primer Seq. 3'

aatctagaggtaccGAGCAATATCATACCCGGGAT

Predicted Fragment Size

IR fragment full Seq

0001 ATGAACAGTC CTCTCGGGAT CACTGCCCTA ATTTGGGGCA TTCTCTGCCT TAGCTGTCCA 
0061 CCTTCTTCTC AAGCCGGAAG GGAAGATTGG ACACCACACG AGCTTCTGGC TTACGAACAG 
0121 TTGACACAAC AGGATTGCGG TGTCCTATCG AATCTGATTC CCGCCCAAAG GCTTCGACGG 
0181 CGGATCACCG GCGGCAGGAA ATCTTCGCTG TTGTCCCAGC CTTGGATGGC TTTTCTCCAC 
0241 ATTTCCGGTG ATATAGAGAT GTGCCGCTGT GGAGGCTCTC TTCTCTCAGA ACTCTTTGTT 
0301 TTGACTGCCN GCGCACTGCT TCAAAATGTG TCCCCGATCT AAGGAGATAA GAGTGTGGCT 
0361 GGGAGAGCTC GACATTAGTT CCACAAGTGA CTGCGTCACC TACAATTATC AACGGGTTTG 
0421 CGCACTGCCC GTGGAGGAGT TTACCATCGA CAAGTGGATT CTTCACGAGG AGTTTAACCT 
0481 CTTCTATCCC GGGTATGATA TTGCTC

in silico PCR Fragment

0001 ATGAACAGTC CTCTCGGGAT CACTGCCCTA ATTTGGGGCA TTCTCTGCCT TAGCTGTCCA 
0061 CCTTCTTCTC AAGCCGGAAG GGAAGATTGG ACACCACACG AGCTTCTGGC TTACGAACAG 
0121 TTGACACAAC AGGATTGCGG TGTCCTATCG AATCTGATTC CCGCCCAAAG GCTTCGACGG 
0181 CGGATCACCG GCGGCAGGAA ATCTTCGCTG TTGTCCCAGC CTTGGATGGC TTTTCTCCAC 
0241 ATTTCCGGTG ATATAGAGAT GTGCCGCTGT GGAGGCTCTC TTCTCTCAGA ACTCTTTGTT 
0301 TTGACTGCC- GCGCACTGCT TCAAAATGTG TCCCCGATCT AAGGAGATAA GAGTGTGGCT 
0361 GGGAGAGCTC GACATTAGTT CCACAAGTGA CTGCGTCACC TACAATTATC AACGGGTTTG 
0421 CGCACTGCCC GTGGAGGAGT TTACCATCGA CAAGTGGATT CTTCACGAGG AGTTTAACCT 
0481 CTTCTATCCC GGGTATGATA TTGCTC

Assemble Data


1773R-1.IR_full       1   ATGAACAGTCCTCTCGGGATCACTGCCCTAATTTGGGGCATTCTCTGCCTTAGCTGTCCA 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     1   ATGAACAGTCCTCTCGGGATCACTGCCCTAATTTGGGGCATTCTCTGCCTTAGCTGTCCA 60


1773R-1.IR_full       61  CCTTCTTCTCAAGCCGGAAGGGAAGATTGGACACCACACGAGCTTCTGGCTTACGAACAG 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     61  CCTTCTTCTCAAGCCGGAAGGGAAGATTGGACACCACACGAGCTTCTGGCTTACGAACAG 120


1773R-1.IR_full       121 TTGACACAACAGGATTGCGGTGTCCTATCGAATCTGATTCCCGCCCAAAGGCTTCGACGG 180
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     121 TTGACACAACAGGATTGCGGTGTCCTATCGAATCTGATTCCCGCCCAAAGGCTTCGACGG 180


1773R-1.IR_full       181 CGGATCACCGGCGGCAGGAAATCTTCGCTGTTGTCCCAGCCTTGGATGGCTTTTCTCCAC 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     181 CGGATCACCGGCGGCAGGAAATCTTCGCTGTTGTCCCAGCCTTGGATGGCTTTTCTCCAC 240


1773R-1.IR_full       241 ATTTCCGGTGATATAGAGATGTGCCGCTGTGGAGGCTCTCTTCTCTCAGAACTCTTTGTT 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     241 ATTTCCGGTGATATAGAGATGTGCCGCTGTGGAGGCTCTCTTCTCTCAGAACTCTTTGTT 300


1773R-1.IR_full       301 TTGACTGCCNGCGCACTGCTTCAAAATGTGTCCCCGATCTAAGGAGATAAGAGTGTGGCT 360
                          ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     301 TTGACTGCC-GCGCACTGCTTCAAAATGTGTCCCCGATCTAAGGAGATAAGAGTGTGGCT 360


1773R-1.IR_full       361 GGGAGAGCTCGACATTAGTTCCACAAGTGACTGCGTCACCTACAATTATCAACGGGTTTG 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     361 GGGAGAGCTCGACATTAGTTCCACAAGTGACTGCGTCACCTACAATTATCAACGGGTTTG 420


1773R-1.IR_full       421 CGCACTGCCCGTGGAGGAGTTTACCATCGACAAGTGGATTCTTCACGAGGAGTTTAACCT 480
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1773R-1.in silico     421 CGCACTGCCCGTGGAGGAGTTTACCATCGACAAGTGGATTCTTCACGAGGAGTTTAACCT 480


1773R-1.IR_full       481 CTTCTATCCCGGGTATGATATTGCTC 506
                          ||||||||||||||||||||||||||
1773R-1.in silico     481 CTTCTATCCCGGGTATGATATTGCTC 506

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	487	0	0	0	NM_136668.2	CG1773-RA (CG1773), mRNA
21.76	106	124	97	47	NM_165691.2	CG30002-RA (CG30002), mRNA
0	0	4	2	3	NM_142222.1	CG5302-RA (CG5302), mRNA
0	0	2	2	2	NM_166992.2	CG2904-RA (ec), mRNA
0	0	1	5	3	NM_206133.1	CG33459-RA (CG33459), mRNA
0	0	1	3	8	NM_139854.1	CG8583-RA (sec63), mRNA
0	0	1	3	3	NM_139880.2	CG7526-RA, transcript variant A (CG7526), mRNA
0	0	1	3	3	NM_206292.1	CG7526-RB, transcript variant B (CG7526), mRNA
0	0	1	3	2	NM_132476.2	CG1657-RA (CG1657), mRNA
0	0	1	2	5	NM_079729.2	CG4792-RA (Dcr-1), mRNA
0	0	1	2	3	NM_140319.1	CG10616-RA (CG10616), mRNA
0	0	1	2	3	NM_139851.1	CG14834-RA (CG14834), mRNA
0	0	0	7	2	NM_170040.2	CG31139-RA (CG31139), mRNA
0	0	0	6	2	NM_140714.3	CG6456-RA (Mip), mRNA
0	0	0	5	4	NM_138008.3	CG13565-RA (CG13565), mRNA
0	0	0	5	4	NM_139531.3	CG32269-RA, transcript variant A (CG32269), mRNA
0	0	0	5	4	NM_168003.2	CG32270-RA, transcript variant A (CG32270), mRNA
0	0	0	4	4	NM_136943.1	CG8550-RA (CG8550), mRNA
0	0	0	4	3	NM_136435.2	CG30502-RA (CG30502), mRNA
0	0	0	4	2	NM_080121.2	CG7719-RA (gwl), mRNA
0	0	0	3	7	NM_078590.2	CG1771-RB, transcript variant B (mew), mRNA
0	0	0	3	7	NM_167354.1	CG1771-RA, transcript variant A (mew), mRNA
0	0	0	3	6	NM_057219.3	CG9042-RA, transcript variant A (Gpdh), mRNA
0	0	0	3	6	NM_057218.3	CG9042-RC, transcript variant C (Gpdh), mRNA
0	0	0	3	6	NM_057217.3	CG9042-RB, transcript variant B (Gpdh), mRNA
0	0	0	3	5	NM_166186.2	CG18471-RA (gprs), mRNA
0	0	0	3	5	NM_132702.2	CG11063-RB (CG11063), mRNA
0	0	0	3	4	NM_140363.2	CG11261-RA (CG11261), mRNA
0	0	0	3	4	NM_164834.1	CG9310-RC, transcript variant C (Hnf4), mRNA
0	0	0	3	4	NM_164833.1	CG9310-RB, transcript variant B (Hnf4), mRNA

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