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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
17239R-2 |
| Symbol |
CG17239 |
Full Name |
CG17239 |
| CG No |
CG17239 |
Old CG No |
CG17239 |
| Synonyms |
SP138, CG17239 |
| Accession No (Link to NCBI) |
NM_164469.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGATTTTCCTGGCCTTCAGCGT |
| Primer Seq. 3' |
aatctagaggtaccGACACTGATCCTGGTCCACAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 NATTTTCCTG GCCTTCAGCG TCACCGTGGT TTCCTCTAAT TGGATTCCGG AACGAATCGT
0061 GGGAGGCGAC TTGATAACCA TTCTATCAGT TNCCCTGGCA GGCTTCCATT CTTAGGCGTG
0121 GACGGTTTCA CTGCGGTGCA GCCNATCNTA CAGTGAAGAC ATCGTCATAA CGGCAGCCCA
0181 CTGCCTCACC GACCGCGAGA CCGAGTTCCT CTCAGTGAGA GTCGGCTCAT CGTTCACATT
0241 CTTCGGTGGT CAGGTGGTGA GGGTATCCAG GGTTCTGTTA CACGAGGAGT ACGACCAGTC
0301 CTGGTCCAAC GACATAGCCG TGATGAGGCT TCAGTCCAAG CTCCGGCTGG GAAGTGCAGT
0361 CAGTGTCATT CCCTTGGCCG ATACTCCTCC AGCCAGTGGA TCACCTGCCA CCGTTTCTGG
0421 ATGGGGAGCC ATTGGTTATA AGAAGAATTA CCCCATATCC ATACTGTCCG CTTCGGTGGA
0481 CATTGTGGAC CAGGATCAGT GTC |
| in silico PCR Fragment |
0001 GATTTTCCTG GCCTTCAGCG TCACCGTGGT TTCCTCTAAT TGGATTCCGG AACGAATCGT
0061 GGGAGGCGAC TTGATAACCA TTCTATCAGT T-CCCTGGCA GGCTTCCATT CTTAGGCTTG
0121 GACGGTTTCA CTGCGGTGCA GCC-ATC-TA CAGTGAAGAC ATCGTCATAA CGGCAGCCCA
0181 CTGCCTCACC GACCGCGAGA CCGAGTTCCT CTCAGTGAGA GTCGGCTCAT CGTTCACATT
0241 CTTCGGTGGT CAGGTGGTGA GGGTATCCAG TGTTCTGTTA CACGAGGAAT ACGACCAGTC
0301 CTGGTCCAAC GACATAGCCG TGATGAGGCT TCAGTCCAAG CTCCGGCTGG GAAGTGCAGT
0361 CAGTGTCATT CCCTTGGCCG ATACTCCCCC GGCCAGTGGA TCACCTGCCA CCGTTTCTGG
0421 ATGGGGAGCC ATTGGTTTTA AGAAGAATTA CCCCATGTCC ATACTGTCCG CTTCGGTGGA
0481 CATTGTGGAC CAGGATCAGT GTC |
| Assemble Data |
17239R-2.IR_full 1 ATTTTCCTGGCCTTCAGCGTCACCGTGGTTTCCTCTAATTGGATTCCGGAACGAATCGT 59
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-2.in silico 1 ATTTTCCTGGCCTTCAGCGTCACCGTGGTTTCCTCTAATTGGATTCCGGAACGAATCGT 59
17239R-2.IR_full 61 GGGAGGCGACTTGATAACCATTCTATCAGTTNCCCTGGCAGGCTTCCATTCTTAGGCGTG 119
||||||||||||||||||||||||||||||| ||||||||||||||||||||||||| ||
17239R-2.in silico 61 GGGAGGCGACTTGATAACCATTCTATCAGTT-CCCTGGCAGGCTTCCATTCTTAGGCTTG 119
17239R-2.IR_full 121 GACGGTTTCACTGCGGTGCAGCCNATCNTACAGTGAAGACATCGTCATAACGGCAGCCCA 179
||||||||||||||||||||||| ||| ||||||||||||||||||||||||||||||||
17239R-2.in silico 121 GACGGTTTCACTGCGGTGCAGCC-ATC-TACAGTGAAGACATCGTCATAACGGCAGCCCA 179
17239R-2.IR_full 181 CTGCCTCACCGACCGCGAGACCGAGTTCCTCTCAGTGAGAGTCGGCTCATCGTTCACATT 239
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-2.in silico 181 CTGCCTCACCGACCGCGAGACCGAGTTCCTCTCAGTGAGAGTCGGCTCATCGTTCACATT 239
17239R-2.IR_full 241 CTTCGGTGGTCAGGTGGTGAGGGTATCCAGGGTTCTGTTACACGAGGAGTACGACCAGTC 299
|||||||||||||||||||||||||||||| ||||||||||||||||| |||||||||||
17239R-2.in silico 241 CTTCGGTGGTCAGGTGGTGAGGGTATCCAGTGTTCTGTTACACGAGGAATACGACCAGTC 299
17239R-2.IR_full 301 CTGGTCCAACGACATAGCCGTGATGAGGCTTCAGTCCAAGCTCCGGCTGGGAAGTGCAGT 359
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17239R-2.in silico 301 CTGGTCCAACGACATAGCCGTGATGAGGCTTCAGTCCAAGCTCCGGCTGGGAAGTGCAGT 359
17239R-2.IR_full 361 CAGTGTCATTCCCTTGGCCGATACTCCTCCAGCCAGTGGATCACCTGCCACCGTTTCTGG 419
||||||||||||||||||||||||||| || |||||||||||||||||||||||||||||
17239R-2.in silico 361 CAGTGTCATTCCCTTGGCCGATACTCCCCCGGCCAGTGGATCACCTGCCACCGTTTCTGG 419
17239R-2.IR_full 421 ATGGGGAGCCATTGGTTATAAGAAGAATTACCCCATATCCATACTGTCCGCTTCGGTGGA 479
||||||||||||||||| |||||||||||||||||| |||||||||||||||||||||||
17239R-2.in silico 421 ATGGGGAGCCATTGGTTTTAAGAAGAATTACCCCATGTCCATACTGTCCGCTTCGGTGGA 479
17239R-2.IR_full 481 CATTGTGGACCAGGATCAGTGTC 502
|||||||||||||||||||||||
17239R-2.in silico 481 CATTGTGGACCAGGATCAGTGTC 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164469.1 |
CG17239-RA (CG17239), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_169839.1 |
CG17836-RA, transcript variant A (CG17836), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_142504.2 |
CG17836-RB, transcript variant B (CG17836), mRNA |
| 0 |
0 |
3 |
4 |
7 |
NM_164472.1 |
CG17242-RA (CG17242), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_169693.1 |
CG4261-RA (Hel89B), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_140569.2 |
CG5841-RA (mib1), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_206627.1 |
CG4068-RB, transcript variant B (CG4068), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_140888.2 |
CG8743-RA (CG8743), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_134871.1 |
CG3131-RA (Duox), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169548.1 |
CG9351-RB, transcript variant B (flfl), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_142047.1 |
CG9351-RA, transcript variant A (flfl), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_169549.1 |
CG9351-RC, transcript variant C (flfl), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_169207.1 |
CG7602-RB, transcript variant B (DNApol-iota), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_141515.2 |
CG7602-RA, transcript variant A (DNApol-iota), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_139960.1 |
CG7170-RA (Jon66Cii), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_142446.2 |
CG7142-RA (CG7142), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_168271.1 |
CG7118-RA (Jon66Ci), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057232.3 |
CG1934-RA (ImpE2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_138127.1 |
CG3650-RA (CG3650), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_079011.2 |
CG6692-RC, transcript variant C (Cp1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166027.1 |
CG6692-RB, transcript variant B (Cp1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_166026.1 |
CG6692-RA, transcript variant A (Cp1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_139833.1 |
CG8616-RA (CG8616), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170038.1 |
CG31151-RB, transcript variant B (wge), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170037.1 |
CG31151-RA, transcript variant A (wge), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001043281.1 |
CG31151-RC, transcript variant C (wge), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166031.1 |
CG8233-RA, transcript variant A (CG8233), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137083.2 |
CG8233-RB, transcript variant B (CG8233), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_166032.1 |
CG8233-RC, transcript variant C (CG8233), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_078671.3 |
CG7098-RA (dik), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| +- |
|
| DM |
 |
| +- |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
| Dcompartment |
|
Penetrance |
| + |
|
|
|
|
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