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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 17195R-4 
 Symbol CG17195  Full Name CG17195 
 CG No CG17195  Old CG No CG17195 
 Synonyms CG17195 
 Accession No (Link to NCBI) NM_143170.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGCCCGAATCCAGAGGATGA 
 Primer Seq. 3' 
aatctagaggtaccAAAGTCCTCGTTGGCCCATTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ATGCCCGAAT CCAGAGGATG AGCCCCTGTG GcACTATTGC GAATCGTGTA AAAAGCTGAG 
0061 ATCGCCACGA TCCTGGCACT GCGTTTTGTG CAACACCTGC ATTTTGAGAC GGGACCACCA 
0121 CTGTATATTC ACGGGCACTT GCATTGGACA CAATAATCAA CGCTTTTTCT TCTGGTTCAC 
0181 ATTTTATTTA ACGCTCGGCT TAGTCACAAG CTTTGCAACA TTTTGTATGT TTATACCTGC 
0241 AGAATGGCGG TAACTTCATG AGTCTCTCGT CTGTGATTTT TAATTTAATA ACTAGGACTT 
0301 TTTTCCAAAA TTATACTGGC AATACTTTTG AGACCATTGC CTTCCTCTTG AATATAAGCG 
0361 CCAGCTATAT GCCTGCCTTT ATGTTGGCAT ATCAAATGCA GATTTTAAGC CAAAACTCTA 
0421 CCTACTATAA TATTTTTGAT TGCACCTACG ATCTGGGTTT CCGAAAGAAC TGCCAGACAA 
0481 TAATGGGCCA ACGAGGACTT T 
 in silico PCR Fragment 
0001 ATGCCCGAAT CCAGAGGATG AGCCCCTGTG GCACTATTGC GAATCGTGTA AAAAGCTGAG 
0061 ATCGCCACGA TCCTGGCACT GCGTTTTGTG CAACACCTGC ATTTTGAGAC GGGACCACCA 
0121 CTGTATATTC ACGGGCACTT GCATTGGACA CAATAATCAA CGCTTTTTCT TCTGGTTCAC 
0181 ATTTTATTTA ACGCTCGGCT TAGTCACAAG CTTTGCAACA TTTTGTATGT TTAT-CCTGC 
0241 AGAATGGCGG TAACTTCATG AGTCTCTCGT CTGTGATTTT TAATTTAATA ACTAGGACTT 
0301 TTTTCCAAAA TTATACTGGC AATACTTTTG AGACCATTGC CTTCCTCTTG AATATAAGCG 
0361 CCAGCTATAT GCCTGCCTTT ATGTTGGCAT ATCAAATGCA GATTTTAAGC CAAAACTCTA 
0421 CCTACTATAA TATTTTTGAT TGCACCTACG ATCTGGGTTT CCGAAAGAAC TGCCAGACAA 
0481 TAATGGGCCA ACGAGGACTT T 
 Assemble Data 

17195R-4.IR_full       1   ATGCCCGAATCCAGAGGATGAGCCCCTGTGGCACTATTGCGAATCGTGTAAAAAGCTGAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     1   ATGCCCGAATCCAGAGGATGAGCCCCTGTGGCACTATTGCGAATCGTGTAAAAAGCTGAG 60


17195R-4.IR_full       61  ATCGCCACGATCCTGGCACTGCGTTTTGTGCAACACCTGCATTTTGAGACGGGACCACCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     61  ATCGCCACGATCCTGGCACTGCGTTTTGTGCAACACCTGCATTTTGAGACGGGACCACCA 120


17195R-4.IR_full       121 CTGTATATTCACGGGCACTTGCATTGGACACAATAATCAACGCTTTTTCTTCTGGTTCAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     121 CTGTATATTCACGGGCACTTGCATTGGACACAATAATCAACGCTTTTTCTTCTGGTTCAC 180


17195R-4.IR_full       181 ATTTTATTTAACGCTCGGCTTAGTCACAAGCTTTGCAACATTTTGTATGTTTATACCTGC 240
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
17195R-4.in silico     181 ATTTTATTTAACGCTCGGCTTAGTCACAAGCTTTGCAACATTTTGTATGTTTAT-CCTGC 240


17195R-4.IR_full       241 AGAATGGCGGTAACTTCATGAGTCTCTCGTCTGTGATTTTTAATTTAATAACTAGGACTT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     241 AGAATGGCGGTAACTTCATGAGTCTCTCGTCTGTGATTTTTAATTTAATAACTAGGACTT 300


17195R-4.IR_full       301 TTTTCCAAAATTATACTGGCAATACTTTTGAGACCATTGCCTTCCTCTTGAATATAAGCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     301 TTTTCCAAAATTATACTGGCAATACTTTTGAGACCATTGCCTTCCTCTTGAATATAAGCG 360


17195R-4.IR_full       361 CCAGCTATATGCCTGCCTTTATGTTGGCATATCAAATGCAGATTTTAAGCCAAAACTCTA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     361 CCAGCTATATGCCTGCCTTTATGTTGGCATATCAAATGCAGATTTTAAGCCAAAACTCTA 420


17195R-4.IR_full       421 CCTACTATAATATTTTTGATTGCACCTACGATCTGGGTTTCCGAAAGAACTGCCAGACAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
17195R-4.in silico     421 CCTACTATAATATTTTTGATTGCACCTACGATCTGGGTTTCCGAAAGAACTGCCAGACAA 480


17195R-4.IR_full       481 TAATGGGCCAACGAGGACTTT 501
                           |||||||||||||||||||||
17195R-4.in silico     481 TAATGGGCCAACGAGGACTTT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_143170.2  CG17195-RA (CG17195), mRNA 
0.2   1  2  2  2  NM_142328.2  CG8907-RA (CG8907), mRNA 
0   0  14  22  47  NM_143169.1  CG17196-RA (CG17196), mRNA 
0   0  3  3  2  NM_136758.2  CG12309-RA (CG12309), mRNA 
0   0  2  2  6  NM_142927.2  CG10214-RA (CG10214), mRNA 
0   0  1  5  18  NM_137009.1  CG4676-RA (CG4676), mRNA 
0   0  1  2  2  NM_165858.2  CG30036-RA (CG30036), mRNA 
0   0  0  6  21  NM_143167.2  CG17198-RA (CG17198), mRNA 
0   0  0  5  5  NM_205883.1  CG4574-RD, transcript variant D (Plc21C), mRNA 
0   0  0  5  5  NM_057503.4  CG4574-RB, transcript variant B (Plc21C), mRNA 
0   0  0  4  29  NM_143168.1  CG17197-RA (CG17197), mRNA 
0   0  0  4  26  NM_140671.1  CG13029-RC, transcript variant C (CG13029), mRNA 
0   0  0  4  11  NM_165610.1  CG8687-RA, transcript variant A (Cyp6a14), mRNA 
0   0  0  4  11  NM_136545.1  CG8687-RB, transcript variant B (Cyp6a14), mRNA 
0   0  0  4  7  NM_132724.2  CG1810-RA (mRNA-capping-enzyme), mRNA 
0   0  0  4  5  NM_001015359.1  CG41099-PC.3 (CG41099), mRNA 
0   0  0  4  5  NM_001015360.1  CG41099-PB.3 (CG41099), mRNA 
0   0  0  4  4  NM_079002.2  CG3905-RA (Su(z)2), mRNA 
0   0  0  3  26  NM_168690.1  CG13029-RB, transcript variant B (CG13029), mRNA 
0   0  0  3  5  NM_132544.1  CG18130-RA (CG18130), mRNA 
0   0  0  3  5  NM_168756.1  CG8127-RC, transcript variant C (Eip75B), mRNA 
0   0  0  3  5  NM_079409.2  CG8127-RA, transcript variant A (Eip75B), mRNA 
0   0  0  3  5  NM_168755.1  CG8127-RB, transcript variant B (Eip75B), mRNA 
0   0  0  3  5  NM_168757.1  CG8127-RD, transcript variant D (Eip75B), mRNA 
0   0  0  3  4  NM_140678.1  CG13023-RA (CG13023), mRNA 
0   0  0  3  4  NM_168906.1  CG5081-RB, transcript variant B (Syx7), mRNA 
0   0  0  3  4  NM_168905.1  CG5081-RA, transcript variant A (Syx7), mRNA 
0   0  0  3  3  NM_001042789.1  CG13366-RB, transcript variant B (CG13366), mRNA 
0   0  0  3  3  NM_140937.2  CG17149-RA, transcript variant A (CG17149), mRNA 
0   0  0  3  3  NM_168835.1  CG17149-RB, transcript variant B (CG17149), mRNA 

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