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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 15333R-4 
 Symbol CG15333  Full Name CG15333 
 CG No CG15333  Old CG No CG15333 
 Synonyms CG18293, CG15333 
 Accession No (Link to NCBI) NM_132203.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTGTGGTGGCCCGCCTATATC 
 Primer Seq. 3' 
aatctagaggtaccACGGCCATATGAATAGGGCGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 TTGTGGTGGC CCGCCTATAT CTCGATGTCA ATAAGCACGA TAAGCAGGCA GCCGAAAACT 
0061 TCGAGGGAAC CGAGACCCCC ATGCTTTGAT GTGCCGCCAT CCCTGTTCGC GGATAAGGTT 
0121 CCAATGGACA AGATTGTCTT TCTGCCAACG TCCATGTTGC CCATGGGCTT CGAAGCTGGC 
0181 GGTGTTTTTG GGCCGGGTAT CCTTACGCGT CGTTCCTATC CCATTGACTT GAAGGCCGCG 
0241 GGTCACAAGG GTCAAACGCC GCCGCTGTTT GTCGGTTTGC GCATGGAAGT CCAACCGCCG 
0301 ACAAGGGTCG AGAGTCTCCT AAAAAAAGAC GGTGAGTCCC AACCAATTCA GGACACATTG 
0361 ATGAATTGGT TGCATGCCAG CAATGATCTT ATAAATGGGG CACAGCCAAA AGGACAAGAG 
0421 CTTCGTGATG AATTTTCGCT ATCGATGGTC TTCAATTTGC CAATACCACC GAGTCCACCA 
0481 TCGCCCTATT CATANGGCCG T 
 in silico PCR Fragment 
0001 TTGTGGTGGC CCGCCTATAT CTCGATGTCA ATAAGCACGA TAAGCAGGCA GCCGAAAACT 
0061 TCGAGGGAAC CGAGA-CCCC ATGCTTTGAT GTGCCGCCAT CCCTGTTCGC GGATAAGGTT 
0121 CCAATGGACA AGATTGTCTT TCTGCCAACG TCCATGTTGC CCATGGGCTT CGAAGCTGGC 
0181 GGTGTTTTTG GGCCGGGTAT CCTTACGCGT CGTTCCTATC CCATTGACTT GAAGGCCGCG 
0241 GGTCACAAGG GTCAAACGCC GCCGCTGTTT GTCGGTTTGC GCATGGAAGT CCAACCGCCG 
0301 ACAAGGGTCG AGAGTCTCCT AAAAAAAGAC GGTGAGTCCC AACCAATTCA GGACACATTG 
0361 ATGAATTGGT TGCATGCCAG CAATGATCTT ATAAATGGGG CACAGCCAAA AGGACAAGAG 
0421 CTTCGTGATG AATTTTCGCT ATCGATGGTC TTCAATTTGC CAATACCACC GAGTCCACCA 
0481 TCGCCCTATT CATATGGCCG T 
 Assemble Data 

15333R-4.IR_full       1   TTGTGGTGGCCCGCCTATATCTCGATGTCAATAAGCACGATAAGCAGGCAGCCGAAAACT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     1   TTGTGGTGGCCCGCCTATATCTCGATGTCAATAAGCACGATAAGCAGGCAGCCGAAAACT 60


15333R-4.IR_full       61  TCGAGGGAACCGAGACCCCCATGCTTTGATGTGCCGCCATCCCTGTTCGCGGATAAGGTT 120
                           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     61  TCGAGGGAACCGAGA-CCCCATGCTTTGATGTGCCGCCATCCCTGTTCGCGGATAAGGTT 120


15333R-4.IR_full       121 CCAATGGACAAGATTGTCTTTCTGCCAACGTCCATGTTGCCCATGGGCTTCGAAGCTGGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     121 CCAATGGACAAGATTGTCTTTCTGCCAACGTCCATGTTGCCCATGGGCTTCGAAGCTGGC 180


15333R-4.IR_full       181 GGTGTTTTTGGGCCGGGTATCCTTACGCGTCGTTCCTATCCCATTGACTTGAAGGCCGCG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     181 GGTGTTTTTGGGCCGGGTATCCTTACGCGTCGTTCCTATCCCATTGACTTGAAGGCCGCG 240


15333R-4.IR_full       241 GGTCACAAGGGTCAAACGCCGCCGCTGTTTGTCGGTTTGCGCATGGAAGTCCAACCGCCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     241 GGTCACAAGGGTCAAACGCCGCCGCTGTTTGTCGGTTTGCGCATGGAAGTCCAACCGCCG 300


15333R-4.IR_full       301 ACAAGGGTCGAGAGTCTCCTAAAAAAAGACGGTGAGTCCCAACCAATTCAGGACACATTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     301 ACAAGGGTCGAGAGTCTCCTAAAAAAAGACGGTGAGTCCCAACCAATTCAGGACACATTG 360


15333R-4.IR_full       361 ATGAATTGGTTGCATGCCAGCAATGATCTTATAAATGGGGCACAGCCAAAAGGACAAGAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     361 ATGAATTGGTTGCATGCCAGCAATGATCTTATAAATGGGGCACAGCCAAAAGGACAAGAG 420


15333R-4.IR_full       421 CTTCGTGATGAATTTTCGCTATCGATGGTCTTCAATTTGCCAATACCACCGAGTCCACCA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
15333R-4.in silico     421 CTTCGTGATGAATTTTCGCTATCGATGGTCTTCAATTTGCCAATACCACCGAGTCCACCA 480


15333R-4.IR_full       481 TCGCCCTATTCATANGGCCGT 501
                           |||||||||||||| ||||||
15333R-4.in silico     481 TCGCCCTATTCATATGGCCGT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_132203.2  CG15333-RA (CG15333), mRNA 
1.03   5  6  51  56  NM_206644.1  CG15332-RB, transcript variant B (CG15332), mRNA 
1.03   5  6  51  56  NM_132201.1  CG15332-RA, transcript variant A (CG15332), mRNA 
0   0  1  6  2  NM_134278.2  CG2621-RC, transcript variant C (sgg), mRNA 
0   0  1  5  6  NM_135831.2  CG7578-RA, transcript variant A (sec71), mRNA 
0   0  1  5  6  NM_165059.1  CG7578-RB, transcript variant B (sec71), mRNA 
0   0  1  2  3  NM_132294.2  CG32707-RA (APC4), mRNA 
0   0  1  2  2  NM_165626.1  CG8740-RB, transcript variant B (CG8740), mRNA 
0   0  1  2  2  NM_165627.1  CG8740-RC, transcript variant C (CG8740), mRNA 
0   0  1  2  2  NM_165512.1  CG11084-RC, transcript variant C (pk), mRNA 
0   0  0  4  5  NM_143229.1  CG6478-RA (CG6478), mRNA 
0   0  0  4  5  NM_170280.1  CG6447-RB, transcript variant B (CG6447), mRNA 
0   0  0  4  5  NM_170279.2  CG6447-RA, transcript variant A (CG6447), mRNA 
0   0  0  4  2  NM_136037.2  CG10275-RA (CG10275), mRNA 
0   0  0  3  5  NM_057612.4  CG3938-RB, transcript variant B (CycE), mRNA 
0   0  0  3  5  NM_165124.2  CG3938-RD, transcript variant D (CycE), mRNA 
0   0  0  3  5  NM_165125.2  CG3938-RE, transcript variant E (CycE), mRNA 
0   0  0  3  5  NM_057611.4  CG3938-RA, transcript variant A (CycE), mRNA 
0   0  0  3  5  NM_165123.2  CG3938-RC, transcript variant C (CycE), mRNA 
0   0  0  3  4  NM_142610.2  CG5023-RA (CG5023), mRNA 
0   0  0  3  3  NM_001038795.1  CG8683-RA (CG8683), mRNA 
0   0  0  3  3  NM_169887.1  CG4390-RB, transcript variant B (CG4390), mRNA 
0   0  0  3  3  NM_142607.1  CG4390-RA, transcript variant A (CG4390), mRNA 
0   0  0  3  3  NM_139440.1  CG13810-RA (CG13810), mRNA 
0   0  0  3  2  NM_136814.1  CG9076-RA (CG9076), mRNA 
0   0  0  3  2  NM_167668.1  CG14206-RB, transcript variant B (RpS10b), mRNA 
0   0  0  3  2  NM_134480.2  CG14206-RC, transcript variant C (RpS10b), mRNA 
0   0  0  2  15  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  2  14  NM_144443.1  CG18858-RA (CG18858), mRNA 
0   0  0  2  14  NM_165330.2  CG31683-RA (CG31683), mRNA 

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