NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14932R-2

Symbol CG14932 Full Name CG14932

CG No CG14932 Old CG No CG14932

Synonyms CG14932

Accession No (Link to NCBI) NM_135673.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal

Map Viewer

Reference
[Please submit your publication] Yano H, Yamamoto-Hino M, Awano W, Aoki-Kinoshita KF, Tsuda-Sakurai K, Okano H, Goto S.
Identification of proteasome components required for apical localization of Chaoptin using functional genomics.
J Neurogenet (2012) 26(1) 53-63 [ PubMed ID = 22417167 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGGGCAGACTGGTCCCCATA

Primer Seq. 3'

aatctagaggtaccCGGCGCATCCTCTTGGATTTC

Predicted Fragment Size

IR fragment full Seq

0001 TGGGGCAGAC TGGTcCCCAT ATGTCACTTA TGAGGCGGGT ACACCAAGAC GTCCCCCACA 
0061 GTGCTTTGGA GATTACCGAA ATTACCGAGT TTCCGAAAAT TCCAAAGAAA AACCCCACCT 
0121 CACCCGAACT GGTGATACAG AAGCCAGAGA CTCCGGATCA AAACGACTCG GGATCTCAGG 
0181 AGCGTCTCAC CTTCTNCTCG CGCCAATAAT GCGCTATCAG AGTCCATTGG AGCTCGCGTT 
0241 TCCCAACGTA GCAGGGGCAA CTTGGAACCA TTCATCCGTA GAATGGCACG TAGCCCTACG 
0301 CCCGAGGCCT CAGAGGAAAA CGGCTCGGTA TCACAAGGTC AGCTGCTGGC CAAGCCCGTG 
0361 GATGTCACCG AGTTCTTTCA NCCGCATTGC TGTGGGTCTC GTCGAGCAGC GCGTCTGCTT 
0421 GAAGCCTTTC TTGGCATTGC GCAAACATAT TGCTCAACTG GTGGGTCAGA CTTTGAAGAA 
0481 ATCCAAGAGG ATGCGCCG

in silico PCR Fragment

0001 TGGGGCAGAC TGGTCCCCAT ATGTCACTTA TGAGGCGGGT ACACCAAGAC GT-CCCCACA 
0061 GTGCTTTGGA GATTACCGAA ATTACCGAGT TTCCGAAAAT TCCAAAGAAA AACCCCACCT 
0121 CACCCGAACT GGTGATACAG AAGCCAGAGA CTCCGGATCA AAACGACTCG GGATCTCAGG 
0181 AGCGTCTCAC CTTCT-CTCG CGCCAATAAT GCGCTATCAG AGTCCATTGG AGCTCGCGTT 
0241 TCCCAACGTA GCAGGGGCAA CTTGGAACCA TTCATCCGTA GAATGGCACG TAGCCCTACG 
0301 CCCGAGGCCT CAGAGGAAAA CGGCTCGGTA TCACAAGGTC AGCTGCTGGC CAAGCCCGTG 
0361 GATGTCACCG AGTTCTTTCA -CCGCATTGC TGTGGGTCTC GTCGAGCAGC GCGTCTGCTT 
0421 GAAGCCTTTC TTGGCATTGC GCAAACATAT TGCTCAACTG GTGGGTCAGA CTTTGAAGAA 
0481 ATCCAAGAGG ATGCGCCG

Assemble Data


14932R-2.IR_full       1   TGGGGCAGACTGGTCCCCATATGTCACTTATGAGGCGGGTACACCAAGACGTCCCCCACA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
14932R-2.in silico     1   TGGGGCAGACTGGTCCCCATATGTCACTTATGAGGCGGGTACACCAAGACGT-CCCCACA 60


14932R-2.IR_full       61  GTGCTTTGGAGATTACCGAAATTACCGAGTTTCCGAAAATTCCAAAGAAAAACCCCACCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     61  GTGCTTTGGAGATTACCGAAATTACCGAGTTTCCGAAAATTCCAAAGAAAAACCCCACCT 120


14932R-2.IR_full       121 CACCCGAACTGGTGATACAGAAGCCAGAGACTCCGGATCAAAACGACTCGGGATCTCAGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     121 CACCCGAACTGGTGATACAGAAGCCAGAGACTCCGGATCAAAACGACTCGGGATCTCAGG 180


14932R-2.IR_full       181 AGCGTCTCACCTTCTNCTCGCGCCAATAATGCGCTATCAGAGTCCATTGGAGCTCGCGTT 240
                           ||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     181 AGCGTCTCACCTTCT-CTCGCGCCAATAATGCGCTATCAGAGTCCATTGGAGCTCGCGTT 240


14932R-2.IR_full       241 TCCCAACGTAGCAGGGGCAACTTGGAACCATTCATCCGTAGAATGGCACGTAGCCCTACG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     241 TCCCAACGTAGCAGGGGCAACTTGGAACCATTCATCCGTAGAATGGCACGTAGCCCTACG 300


14932R-2.IR_full       301 CCCGAGGCCTCAGAGGAAAACGGCTCGGTATCACAAGGTCAGCTGCTGGCCAAGCCCGTG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     301 CCCGAGGCCTCAGAGGAAAACGGCTCGGTATCACAAGGTCAGCTGCTGGCCAAGCCCGTG 360


14932R-2.IR_full       361 GATGTCACCGAGTTCTTTCANCCGCATTGCTGTGGGTCTCGTCGAGCAGCGCGTCTGCTT 420
                           |||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     361 GATGTCACCGAGTTCTTTCA-CCGCATTGCTGTGGGTCTCGTCGAGCAGCGCGTCTGCTT 420


14932R-2.IR_full       421 GAAGCCTTTCTTGGCATTGCGCAAACATATTGCTCAACTGGTGGGTCAGACTTTGAAGAA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14932R-2.in silico     421 GAAGCCTTTCTTGGCATTGCGCAAACATATTGCTCAACTGGTGGGTCAGACTTTGAAGAA 480


14932R-2.IR_full       481 ATCCAAGAGGATGCGCCG 498
                           ||||||||||||||||||
14932R-2.in silico     481 ATCCAAGAGGATGCGCCG 498

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	477	0	0	2	NM_135673.1	CG14932-RA (CG14932), mRNA
0.2	1	2	5	2	NM_170379.2	CG31048-RA (CG31048), mRNA
0	0	2	2	3	NM_080146.2	CG10772-RB, transcript variant B (Fur1), mRNA
0	0	1	5	8	NM_133159.1	CG14200-RA (CG14200), mRNA
0	0	1	3	5	NM_137879.2	CG9825-RA (CG9825), mRNA
0	0	1	3	3	NM_140985.2	CG4825-RA (CG4825), mRNA
0	0	1	2	6	NM_141005.1	CG3618-RA (CG3618), mRNA
0	0	1	2	2	NM_143138.1	CG5127-RA (CG5127), mRNA
0	0	1	2	2	NM_143730.2	CG8186-RA (Vha36), mRNA
0	0	1	2	2	NM_167474.1	CG8909-RB (CG8909), mRNA
0	0	0	5	7	NM_166992.2	CG2904-RA (ec), mRNA
0	0	0	5	6	NM_137100.2	CG8503-RA (CG8503), mRNA
0	0	0	5	5	NM_138254.2	CG9153-RB, transcript variant B (CG9153), mRNA
0	0	0	5	5	NM_167868.1	CG9153-RA, transcript variant A (CG9153), mRNA
0	0	0	5	4	NM_136055.2	CG10376-RA (CG10376), mRNA
0	0	0	5	4	NM_138192.1	CG13891-RA (CG13891), mRNA
0	0	0	4	9	NM_131924.2	CG4857-RB (CG4857), mRNA
0	0	0	4	5	NM_135604.2	CG12299-RA (CG12299), mRNA
0	0	0	4	4	NM_141040.1	CG7752-RA (Z4), mRNA
0	0	0	4	3	NM_057245.2	CG6189-RA (l(1)1Bi), mRNA
0	0	0	4	3	NM_135023.1	CG11927-RA (CG11927), mRNA
0	0	0	3	6	NM_136130.3	CG18094-RA (CG18094), mRNA
0	0	0	3	5	NM_135800.2	CG6565-RA (CG6565), mRNA
0	0	0	3	5	NM_001042936.1	CG40006-RA (CG40006), mRNA
0	0	0	3	4	NM_130497.2	CG13363-RA (Suv4-20), mRNA
0	0	0	3	3	NM_206195.1	CG33226-RA (CG33226), mRNA
0	0	0	3	3	NM_079839.2	CG7929-RA (ocn), mRNA
0	0	0	3	2	NM_139472.2	CG16984-RA (CG16984), mRNA
0	0	0	3	2	NM_079861.2	CG1455-RA (CanA1), mRNA
0	0	0	3	2	NM_170430.2	CG31037-RA (ca), mRNA

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