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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14719R-2

Symbol I-t Full Name inhibitor-t

CG No CG14719 Old CG No CG14719

Synonyms pp1d6, CG14719, I-t

Accession No (Link to NCBI) NM_079599.3

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTATGAAGAAGGCCAAAACCGC

Primer Seq. 3'

aatctagaggtaccATGTTGTTCTGGTCGCAGAAG

Predicted Fragment Size

IR fragment full Seq

0001 TATGAAGAAG GCCAAAACCG CGGCTAAGGG ATTTCCCATT CACCTTGGTT TTTATAGGAA 
0061 CCCCAGCGAG TATGACGATC GCACGACCAC CAATCGCGAT TATGGATTGA AAAGTTCCAA 
0121 GGGTTCTGGA AACGCCATTT CCGCAAAAAC AAAAGAAATT AGATACGGCG GCACTAACGG 
0181 CCAAGTTGGA GACGGATAGT CCGCTCCGAT CTGAATTAAG CGACTTATCG ATTGTCATCT 
0241 CCGAAAGAAA GGCGTCCTCA GAACCACAAG TGGCATCCAA TACTACAACC GAACCAACGT 
0301 TTGAGATGAG ACGAAAGCTT TTTGACGAGG CCGAGTTCAC CATCTGCAAG GGTCACAAAC 
0361 TGAACCAGGA CTTCCATCAT ATAGTCGAAG ATGAAAAGCA TTTTAACAAA AGGCAGAGTG 
0421 CCAAGGATAA CATTCCCTAT AGCAATTTCA TGGATCTGAA GAACTTCTGC GACCAGAACA 
0481 ACAT

in silico PCR Fragment

0001 TATGAAGAAG GCCAAAACCG CGGCTAAGGG ATTTCCCATT CACCTTGGTT TTTATAGGAA 
0061 CCCCAGCGAG TATGACGATC GCACGACCAC CAATCGCGAT TATGGATTGA AAAGTTCCAA 
0121 GGGTTCTGG- AACGCCATTT CCGCAAAAAC AAAAGAAATT AGATACGGCG GCACTAACGG 
0181 CCAAGTTGGA GACGGATAGT CTGCTCCGAT CTGAATTAAG CGACTTATCG ATTGTCATCT 
0241 CCGAAAGAAA GGCGTCCTCA GAACCACAAG TGGCATCCAA TACTACAACC GAACCAACGT 
0301 TTGAGATGAG ACGAAAGCTT TTTGACGAGG CCGAGTTCAC CATCTGCAAG GGTCACAAAC 
0361 TGAACCAGGA CTTCCATCAT ATAGTCGAAG ATGAAAAGCA TTTTAACAAA AGGCAGAGTG 
0421 CCAAGGATAA CATTCCCTAT AGCAATTTCA TGGATCTGAA GAACTTCTGC GACCAGAACA 
0481 ACAT

Assemble Data


14719R-2.IR_full       1   TATGAAGAAGGCCAAAACCGCGGCTAAGGGATTTCCCATTCACCTTGGTTTTTATAGGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     1   TATGAAGAAGGCCAAAACCGCGGCTAAGGGATTTCCCATTCACCTTGGTTTTTATAGGAA 60


14719R-2.IR_full       61  CCCCAGCGAGTATGACGATCGCACGACCACCAATCGCGATTATGGATTGAAAAGTTCCAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     61  CCCCAGCGAGTATGACGATCGCACGACCACCAATCGCGATTATGGATTGAAAAGTTCCAA 120


14719R-2.IR_full       121 GGGTTCTGGAAACGCCATTTCCGCAAAAACAAAAGAAATTAGATACGGCGGCACTAACGG 180
                           ||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     121 GGGTTCTGG-AACGCCATTTCCGCAAAAACAAAAGAAATTAGATACGGCGGCACTAACGG 180


14719R-2.IR_full       181 CCAAGTTGGAGACGGATAGTCCGCTCCGATCTGAATTAAGCGACTTATCGATTGTCATCT 240
                           ||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     181 CCAAGTTGGAGACGGATAGTCTGCTCCGATCTGAATTAAGCGACTTATCGATTGTCATCT 240


14719R-2.IR_full       241 CCGAAAGAAAGGCGTCCTCAGAACCACAAGTGGCATCCAATACTACAACCGAACCAACGT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     241 CCGAAAGAAAGGCGTCCTCAGAACCACAAGTGGCATCCAATACTACAACCGAACCAACGT 300


14719R-2.IR_full       301 TTGAGATGAGACGAAAGCTTTTTGACGAGGCCGAGTTCACCATCTGCAAGGGTCACAAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     301 TTGAGATGAGACGAAAGCTTTTTGACGAGGCCGAGTTCACCATCTGCAAGGGTCACAAAC 360


14719R-2.IR_full       361 TGAACCAGGACTTCCATCATATAGTCGAAGATGAAAAGCATTTTAACAAAAGGCAGAGTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     361 TGAACCAGGACTTCCATCATATAGTCGAAGATGAAAAGCATTTTAACAAAAGGCAGAGTG 420


14719R-2.IR_full       421 CCAAGGATAACATTCCCTATAGCAATTTCATGGATCTGAAGAACTTCTGCGACCAGAACA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14719R-2.in silico     421 CCAAGGATAACATTCCCTATAGCAATTTCATGGATCTGAAGAACTTCTGCGACCAGAACA 480


14719R-2.IR_full       481 ACAT 484
                           ||||
14719R-2.in silico     481 ACAT 484

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	465	0	0	1	NM_079599.3	CG14719-RA (I-t), mRNA
0	0	4	3	4	NM_133104.2	CG7282-RA (CG7282), mRNA
0	0	4	2	7	NM_140985.2	CG4825-RA (CG4825), mRNA
0	0	2	4	2	NM_136623.1	CG8014-RA (Rme-8), mRNA
0	0	2	3	5	NM_138049.2	CG3328-RA (CG3328), mRNA
0	0	2	3	3	NM_132134.2	CG4558-RA (CG4558), mRNA
0	0	2	2	9	NM_139522.1	CG14964-RA (CG14964), mRNA
0	0	2	2	5	NM_058148.3	CG2637-RA (Fs(2)Ket), mRNA
0	0	2	2	4	NM_079884.3	CG1449-RA (zfh2), mRNA
0	0	1	3	3	NM_079068.2	CG11001-RA (FK506-bp2), mRNA
0	0	1	2	3	NM_138760.2	CG5304-RA (l(2)01810), mRNA
0	0	0	8	2	NM_134726.2	CG4715-RA (Iris), mRNA
0	0	0	6	13	NM_141947.1	CG5509-RA (CG5509), mRNA
0	0	0	6	5	NM_001032244.1	CG32904-RA, transcript variant A (seq), mRNA
0	0	0	4	6	NM_057643.2	CG10844-RA, transcript variant A (Rya-r44F), mRNA
0	0	0	4	6	NM_057644.2	CG10844-RB, transcript variant B (Rya-r44F), mRNA
0	0	0	4	6	NM_057645.2	CG10844-RC, transcript variant C (Rya-r44F), mRNA
0	0	0	4	6	NM_057646.2	CG10844-RD, transcript variant D (Rya-r44F), mRNA
0	0	0	4	4	NM_079539.1	CG1279-RA (Rtnl2), mRNA
0	0	0	4	3	NM_135955.1	CG4631-RA (CG4631), mRNA
0	0	0	4	2	NM_139969.2	CG7015-RA (Unr), mRNA
0	0	0	4	1	NM_132963.1	CG16700-RA (CG16700), mRNA
0	0	0	3	6	NM_134601.1	CG1718-RA (CG1718), mRNA
0	0	0	3	5	NM_166791.1	CG1793-RB, transcript variant B (MED26), mRNA
0	0	0	3	5	NM_143679.3	CG1793-RA, transcript variant A (MED26), mRNA
0	0	0	3	4	NM_170484.1	CG1469-RC, transcript variant C (Fer2LCH), mRNA
0	0	0	3	4	NM_142837.2	CG17141-RA (CG17141), mRNA
0	0	0	3	3	NM_136941.2	CG8828-RA (CG8828), mRNA
0	0	0	3	3	NM_078698.2	CG1676-RA (cactin), mRNA
0	0	0	3	3	NM_176202.1	CG4945-RB, transcript variant B (CG4945), mRNA

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