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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 14708R-3 
 Symbol CG14708  Full Name CG14708 
 CG No CG14708  Old CG No CG14708 
 Synonyms CG14708 
 Accession No (Link to NCBI) NM_141825.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGAGCTGTCGAGTCTCTACAA 
 Primer Seq. 3' 
aatctagaggtaccTAGAGCGGTCGACCAAACGGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGAGCTGTCG AGTCTCTACA ATATCGACAA GTTGACCGAC GAGGAGCTGG CGTCCGTGAA 
0061 TCTGGATAGG AGCTCCCTGA TCGATGACTA TCGTCTACTG CACGAGGTGT CGCAGATGAA 
0121 GCAAATAGAG AATGAAGCAA ATAATATCCC TGCTGCTGCT CCAGCAGGAC AACCAAAATC 
0181 CAAGGAGTTG CATGAGCAAT TCGTAAAAGA GATGGAGAAG TACCTTACCA ATAAGAAGTA 
0241 CACATTTCCG ATGCTCAACC ATTTGATCAA GTTAATGAGT TGGGAAGTGG AGCCGGAGAC 
0301 CATTTTCAAC GAAAAGGCGT TTCAGATTGA TGTCAATTAC CATGAGGACG AGGAGGTAAT 
0361 GCAGCTGGAG CCCAATGAGC GTCTGTTTCG TCGCCTCAAG TCCATCTACG ATCATCTGGA 
0421 GGACATGTTC ATCAACGGCA ATCGCAGAAC GGCCTCCGAC CTGGTCTTCA CCGCAATTAT 
0481 CCGTTTGGTC GACCGCTCTA  
 in silico PCR Fragment 
0001 CGAGCTGTCG AGTCTCTACA ATATCGACAA GTTGACCGAC GAGGAGCTGG CGTCCGTGAA 
0061 TCTGGATAGG AGCTCCCTGA TCGATGACTA TCGTCTACTG CACGAGGTGT CGCAGATGAA 
0121 GCAAATAGAG AATGAAGCAA ATAATATCCC TGCTGCTGCT CCAGCAGGAC AACCAAAATC 
0181 CAAGGAGTTG CATGAGCAAT TCGTAAAAGA GATGGAGAAG TACCTTACCA ATAAGAAGTA 
0241 CACATTTCCG ATGCTCAACC ATTTGATCAA GTTAATGAGT TGGGAAGTGG AGCCGGAGAC 
0301 CATTTTCAAC GAAAAGGCGT TTCAGATTGA TGTCAATTAC CATGAGGACG AGGAGGTAAT 
0361 GCAGCTGGAG CCCAATGAGC GTCTGTTTCG TCGCCTCAAG TCCATCTACG ATCATCTGGA 
0421 GGACATGTTC ATCAACGGCA ATCGCAGAAC GGCCTCCGAC CTGGTCTTCA CCGCAATTAT 
0481 CCGTTTGGTC GACCGCTCTA  
 Assemble Data 

14708R-3.IR_full       1   CGAGCTGTCGAGTCTCTACAATATCGACAAGTTGACCGACGAGGAGCTGGCGTCCGTGAA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     1   CGAGCTGTCGAGTCTCTACAATATCGACAAGTTGACCGACGAGGAGCTGGCGTCCGTGAA 60


14708R-3.IR_full       61  TCTGGATAGGAGCTCCCTGATCGATGACTATCGTCTACTGCACGAGGTGTCGCAGATGAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     61  TCTGGATAGGAGCTCCCTGATCGATGACTATCGTCTACTGCACGAGGTGTCGCAGATGAA 120


14708R-3.IR_full       121 GCAAATAGAGAATGAAGCAAATAATATCCCTGCTGCTGCTCCAGCAGGACAACCAAAATC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     121 GCAAATAGAGAATGAAGCAAATAATATCCCTGCTGCTGCTCCAGCAGGACAACCAAAATC 180


14708R-3.IR_full       181 CAAGGAGTTGCATGAGCAATTCGTAAAAGAGATGGAGAAGTACCTTACCAATAAGAAGTA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     181 CAAGGAGTTGCATGAGCAATTCGTAAAAGAGATGGAGAAGTACCTTACCAATAAGAAGTA 240


14708R-3.IR_full       241 CACATTTCCGATGCTCAACCATTTGATCAAGTTAATGAGTTGGGAAGTGGAGCCGGAGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     241 CACATTTCCGATGCTCAACCATTTGATCAAGTTAATGAGTTGGGAAGTGGAGCCGGAGAC 300


14708R-3.IR_full       301 CATTTTCAACGAAAAGGCGTTTCAGATTGATGTCAATTACCATGAGGACGAGGAGGTAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     301 CATTTTCAACGAAAAGGCGTTTCAGATTGATGTCAATTACCATGAGGACGAGGAGGTAAT 360


14708R-3.IR_full       361 GCAGCTGGAGCCCAATGAGCGTCTGTTTCGTCGCCTCAAGTCCATCTACGATCATCTGGA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     361 GCAGCTGGAGCCCAATGAGCGTCTGTTTCGTCGCCTCAAGTCCATCTACGATCATCTGGA 420


14708R-3.IR_full       421 GGACATGTTCATCAACGGCAATCGCAGAACGGCCTCCGACCTGGTCTTCACCGCAATTAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14708R-3.in silico     421 GGACATGTTCATCAACGGCAATCGCAGAACGGCCTCCGACCTGGTCTTCACCGCAATTAT 480


14708R-3.IR_full       481 CCGTTTGGTCGACCGCTCTA 500
                           ||||||||||||||||||||
14708R-3.in silico     481 CCGTTTGGTCGACCGCTCTA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_141825.1  CG14708-RA (CG14708), mRNA 
0.2   1  23  16  31  NM_131921.1  CG15375-RA (CG15375), mRNA 
0.2   1  2  3  7  NM_132131.2  CG14435-RA (CG14435), mRNA 
0   0  5  2  3  NM_141907.2  CG10014-RA (CG10014), mRNA 
0   0  3  6  3  NM_136909.2  CG13167-RA (CG13167), mRNA 
0   0  3  3  5  NM_175965.1  CG11924-RC, transcript variant C (Cf2), mRNA 
0   0  3  3  5  NM_175963.1  CG11924-RD, transcript variant D (Cf2), mRNA 
0   0  3  3  5  NM_078750.2  CG11924-RA, transcript variant A (Cf2), mRNA 
0   0  3  3  5  NM_175964.1  CG11924-RB, transcript variant B (Cf2), mRNA 
0   0  3  3  2  NM_134893.2  CG17223-RA (alpha4GT1), mRNA 
0   0  3  2  2  NM_134940.1  CG15414-RA (CG15414), mRNA 
0   0  2  6  12  NM_137355.1  CG10953-RA (CG10953), mRNA 
0   0  2  2  2  NM_132958.1  CG12433-RA (CG12433), mRNA 
0   0  1  7  7  NM_001043262.1  CG34157-RE, transcript variant E (Dys), mRNA 
0   0  1  5  6  NM_132413.1  CG15295-RA (CG15295), mRNA 
0   0  1  3  6  NM_130523.2  CG11638-RA (CG11638), mRNA 
0   0  1  3  5  NM_137194.1  CG8179-RA (CG8179), mRNA 
0   0  1  3  4  NM_001038883.1  CG33988-RA (CG33988), mRNA 
0   0  1  3  2  NM_137760.2  CG10306-RA (CG10306), mRNA 
0   0  1  2  10  NM_167829.1  CG6936-RB, transcript variant B (mth), mRNA 
0   0  1  2  10  NM_079147.2  CG6936-RA, transcript variant A (mth), mRNA 
0   0  1  2  6  NM_142755.1  CG5732-RA (CG5732), mRNA 
0   0  1  2  5  NM_166019.1  CG18076-RH, transcript variant H (shot), mRNA 
0   0  1  2  4  NM_169336.2  CG6281-RB, transcript variant B (Timp), mRNA 
0   0  1  2  4  NM_079577.3  CG6281-RA, transcript variant A (Timp), mRNA 
0   0  1  2  3  NM_135338.1  CG8673-RA (CG8673), mRNA 
0   0  0  8  4  NM_079308.2  CG18593-RA (viaf), mRNA 
0   0  0  6  8  NM_001043257.1  CG34157-RH, transcript variant H (Dys), mRNA 
0   0  0  6  8  NM_001043263.1  CG34157-RF, transcript variant F (Dys), mRNA 
0   0  0  6  8  NM_001043259.1  CG34157-RC, transcript variant C (Dys), mRNA 

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