NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 14610R-1

Symbol CG14610 Full Name CG14610

CG No CG14610 Old CG No CG14610

Synonyms CG14610

Accession No (Link to NCBI) NM_141441.1

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAACATGAAACTTTGCGAGGCG

Primer Seq. 3'

aatctagaggtaccAGATTCATTCGGGTCTCTGTG

Predicted Fragment Size

IR fragment full Seq

0001 AACATGAAAC TTTGCGAGGC GGAAAGGATA TACGCGCTCT CGAACAGTGT CGGAAGAATC 
0061 CTGGATGACG CGGACGCCCG AAAGATCCTA AGGCACTTCA TGAAGAGCGA AAATAAGTCC 
0121 ATGCAGTGCG TGGACATCTA TGAGAAGTGC GCAGAGTTTC TTGTGGAGCA CCCGAAGTAC 
0181 GAGTCCTGCC ACTTTGAAGT CCTGGTCCGA CTCGGATTGC CCTCCCATCT GAAGCAGCGT 
0241 CTATTTTATC GATTAAACAA TGCTGATCCC ATTTCCATCT GCCTCTGCCT TAAACACATC 
0301 CAGGAGGAAT GTCTCAGTGA GGTGGCCGAG TCATTCAGCG CCTTCAAGGA ATCTATCACA 
0361 GAGACCCGAA TGAATCT

in silico PCR Fragment

0001 AACATGAAAC TTTGCGAGGC GGAAAGGATA TACGCGCTCT CGAACAGTGT CGGAAGAATC 
0061 CTGGATGACG CGGAGGCCCG AAAGATCCTA AGGCACTTCA TGAAGAGCGA AAATAAGTCC 
0121 ATGCAGTGCG TGGACATCTA TGAGAAGTGC GCAGAGTTTC TTGTGGAGCA CCCGAAGTAC 
0181 GAGTCCTGCC ACTTTGAAGT CCTGGTCCGA CTCGGATTGC CCTCCCATCT GAAGCAGCGT 
0241 CTATTTTATC GATTAAACAA TGCTGATCCC ATTTCCATCT GCCTCTGCCT TAAACACATC 
0301 CAGGAGGAAT GTCTCAGTGA GGTGGCCGAG TCATTCAGCG CCTTCAAGGA ATCTATCACA 
0361 GAGACCCGAA TGAATCT

Assemble Data


14610R-1.IR_full       1   AACATGAAACTTTGCGAGGCGGAAAGGATATACGCGCTCTCGAACAGTGTCGGAAGAATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     1   AACATGAAACTTTGCGAGGCGGAAAGGATATACGCGCTCTCGAACAGTGTCGGAAGAATC 60


14610R-1.IR_full       61  CTGGATGACGCGGACGCCCGAAAGATCCTAAGGCACTTCATGAAGAGCGAAAATAAGTCC 120
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     61  CTGGATGACGCGGAGGCCCGAAAGATCCTAAGGCACTTCATGAAGAGCGAAAATAAGTCC 120


14610R-1.IR_full       121 ATGCAGTGCGTGGACATCTATGAGAAGTGCGCAGAGTTTCTTGTGGAGCACCCGAAGTAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     121 ATGCAGTGCGTGGACATCTATGAGAAGTGCGCAGAGTTTCTTGTGGAGCACCCGAAGTAC 180


14610R-1.IR_full       181 GAGTCCTGCCACTTTGAAGTCCTGGTCCGACTCGGATTGCCCTCCCATCTGAAGCAGCGT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     181 GAGTCCTGCCACTTTGAAGTCCTGGTCCGACTCGGATTGCCCTCCCATCTGAAGCAGCGT 240


14610R-1.IR_full       241 CTATTTTATCGATTAAACAATGCTGATCCCATTTCCATCTGCCTCTGCCTTAAACACATC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     241 CTATTTTATCGATTAAACAATGCTGATCCCATTTCCATCTGCCTCTGCCTTAAACACATC 300


14610R-1.IR_full       301 CAGGAGGAATGTCTCAGTGAGGTGGCCGAGTCATTCAGCGCCTTCAAGGAATCTATCACA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
14610R-1.in silico     301 CAGGAGGAATGTCTCAGTGAGGTGGCCGAGTCATTCAGCGCCTTCAAGGAATCTATCACA 360


14610R-1.IR_full       361 GAGACCCGAATGAATCT 377
                           |||||||||||||||||
14610R-1.in silico     361 GAGACCCGAATGAATCT 377

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	359	0	0	1	NM_141441.1	CG14610-RA (CG14610), mRNA
0	0	6	11	9	NM_143322.1	CG12883-RA (CG12883), mRNA
0	0	5	5	2	NM_136388.2	CG3268-RA (phtf), mRNA
0	0	4	2	2	NM_167013.1	CG3000-RB, transcript variant B (rap), mRNA
0	0	4	2	2	NM_080113.2	CG3000-RA, transcript variant A (rap), mRNA
0	0	1	2	5	NM_168420.1	CG32067-RA, transcript variant A (simj), mRNA
0	0	1	2	5	NM_140150.2	CG32067-RC, transcript variant C (simj), mRNA
0	0	1	2	5	NM_168421.1	CG32067-RB, transcript variant B (simj), mRNA
0	0	1	2	2	NM_205921.1	CG13788-RB, transcript variant B (Gr28b), mRNA
0	0	1	2	2	NM_206099.1	CG8878-RB, transcript variant B (CG8878), mRNA
0	0	1	2	2	NM_136889.2	CG8878-RA, transcript variant A (CG8878), mRNA
0	0	0	6	13	NM_205875.1	CG32019-RE, transcript variant E (bt), mRNA
0	0	0	6	13	NM_205876.1	CG32019-RC, transcript variant C (bt), mRNA
0	0	0	6	13	NM_166790.2	CG32019-RA, transcript variant A (bt), mRNA
0	0	0	6	13	NM_205877.1	CG32019-RD, transcript variant D (bt), mRNA
0	0	0	5	5	NM_167646.1	CG32827-RA (CG32827), mRNA
0	0	0	4	2	NM_001032268.1	CG9218-RF, transcript variant F (sm), mRNA
0	0	0	4	2	NM_057383.5	CG9218-RA, transcript variant A (sm), mRNA
0	0	0	4	2	NM_166368.3	CG9218-RC, transcript variant C (sm), mRNA
0	0	0	4	2	NM_130549.2	CG12773-RA (CG12773), mRNA
0	0	0	3	5	NM_137620.2	CG8908-RB, transcript variant B (CG8908), mRNA
0	0	0	3	5	NM_166385.2	CG8908-RA, transcript variant A (CG8908), mRNA
0	0	0	3	4	NM_141254.1	CG2022-RA (CG2022), mRNA
0	0	0	3	4	NM_166689.1	CG3629-RB, transcript variant B (Dll), mRNA
0	0	0	3	4	NM_143182.2	CG5913-RA (CG5913), mRNA
0	0	0	3	4	NM_079133.1	CG3629-RA, transcript variant A (Dll), mRNA
0	0	0	3	3	NM_140725.1	CG18265-RA (CG18265), mRNA
0	0	0	3	3	NM_001043095.1	CG15078-RB, transcript variant B (Mctp), mRNA
0	0	0	3	2	NM_142423.2	CG7215-RA, transcript variant A (CG7215), mRNA
0	0	0	3	2	NM_142422.3	CG7217-RA, transcript variant A (CG7217), mRNA

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