NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 12721R-3

Symbol CG12721 Full Name CG12721

CG No CG12721 Old CG No CG12721

Synonyms BcDNA:RE60404, CG12721

Accession No (Link to NCBI) NM_132556.1

Inserted Chr. ll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGGATGGCGAAGCTGGCCAA

Primer Seq. 3'

aatctagaggtaccCATTTCCTGGCCTGCGACCAC

Predicted Fragment Size

IR fragment full Seq

0001 ATGGATGGCC GAAGCTGGCC AACCGACAGC CGGAATCGAA TCCCCTGCCG CCAAAGCCAA 
0061 AGAAGCGTAA GAAAATCGCT CCAAAGAAGC AGGACCAGCC TGTCGAGGAG AAGGGCAGCG 
0121 AAGTCGACAT CTACGATGTG GACGACAACG AGATGGTCAA CAATCGCATC TACATTNCCG 
0181 CGACTGcGTT CCGCTTGGAG AGCTCGCTAT TTCAACATTA TGATGCGCGC CTTTGTCATC 
0241 AAACGGGGAC TATTGAATAA GCACATTAAC TGGCAGATTC TCGACAAGAT CATGGCAGCC 
0301 CCCGACGCAG AGGGACAAGC CGAGCTTCAG GCGTTTGTTG ACAAAGTGGT CGCAGGCCAG 
0361 GAAATG

in silico PCR Fragment

0001 ATGGATGG-C GAAGCTGGCC AACCGACAGC CGGAATCGAA TCCCCTGCCG CCAAAGCCAA 
0061 AGAAGCGTAA GAAAATCGCT CCAAAGAAGC AGGACCAGCC TGTCGAGGAG AAGGGCAGCG 
0121 AAGTCGACAT CTACGATGTG GACGACAACG AGATGGTCAA CAATCGCATC TACATT-CCG 
0181 CGACTGCGTT CCGCTTGGAG AGCTCGCTAT TTCAACATTA TGATGCGCGC CTTTGTCATC 
0241 AAACGGGGAC TATTGAATAA GCACATTAAC TGGCAGATTC TCGACAAGAT CATGGCAGCC 
0301 CCCGACGCAG AGGGACAAGC CGAGCTTCAG GCGTTTGTTG ACAAAGTGGT CGCAGGCCAG 
0361 GAAATG

Assemble Data


12721R-3.IR_full       1   ATGGATGGCCGAAGCTGGCCAACCGACAGCCGGAATCGAATCCCCTGCCGCCAAAGCCAA 60
                           |||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||
12721R-3.in silico     1   ATGGATGG-CGAAGCTGGCCAACCGACAGCCGGAATCGAATCCCCTGCCGCCAAAGCCAA 60


12721R-3.IR_full       61  AGAAGCGTAAGAAAATCGCTCCAAAGAAGCAGGACCAGCCTGTCGAGGAGAAGGGCAGCG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12721R-3.in silico     61  AGAAGCGTAAGAAAATCGCTCCAAAGAAGCAGGACCAGCCTGTCGAGGAGAAGGGCAGCG 120


12721R-3.IR_full       121 AAGTCGACATCTACGATGTGGACGACAACGAGATGGTCAACAATCGCATCTACATTNCCG 180
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
12721R-3.in silico     121 AAGTCGACATCTACGATGTGGACGACAACGAGATGGTCAACAATCGCATCTACATT-CCG 180


12721R-3.IR_full       181 CGACTGCGTTCCGCTTGGAGAGCTCGCTATTTCAACATTATGATGCGCGCCTTTGTCATC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12721R-3.in silico     181 CGACTGCGTTCCGCTTGGAGAGCTCGCTATTTCAACATTATGATGCGCGCCTTTGTCATC 240


12721R-3.IR_full       241 AAACGGGGACTATTGAATAAGCACATTAACTGGCAGATTCTCGACAAGATCATGGCAGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12721R-3.in silico     241 AAACGGGGACTATTGAATAAGCACATTAACTGGCAGATTCTCGACAAGATCATGGCAGCC 300


12721R-3.IR_full       301 CCCGACGCAGAGGGACAAGCCGAGCTTCAGGCGTTTGTTGACAAAGTGGTCGCAGGCCAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12721R-3.in silico     301 CCCGACGCAGAGGGACAAGCCGAGCTTCAGGCGTTTGTTGACAAAGTGGTCGCAGGCCAG 360


12721R-3.IR_full       361 GAAATG 366
                           ||||||
12721R-3.in silico     361 GAAATG 366

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	346	0	0	0	NM_132556.1	CG12721-RA (CG12721), mRNA
0	0	4	4	2	NM_170030.1	CG5248-RD, transcript variant D (loco), mRNA
0	0	4	4	2	NM_170031.1	CG5248-RA, transcript variant A (loco), mRNA
0	0	4	4	2	NM_170032.2	CG5248-RB, transcript variant B (loco), mRNA
0	0	4	4	2	NM_170033.1	CG5248-RC, transcript variant C (loco), mRNA
0	0	4	3	6	NM_142683.2	CG7044-RA (CG7044), mRNA
0	0	2	7	2	NM_164537.1	CG8844-RB, transcript variant B (Pdsw), mRNA
0	0	2	7	2	NM_143715.2	CG8844-RA, transcript variant A (Pdsw), mRNA
0	0	2	5	3	NM_143561.2	CG1342-RA (CG1342), mRNA
0	0	2	3	3	NM_079189.3	CG32256-RA (Gr64c), mRNA
0	0	2	3	3	NM_165346.2	CG31673-RA (CG31673), mRNA
0	0	2	2	5	NM_141568.2	CG9797-RA (CG9797), mRNA
0	0	1	4	6	NM_133030.2	CG6842-RA (CG6842), mRNA
0	0	1	4	6	NM_206106.1	CG4616-RB, transcript variant B (CG4616), mRNA
0	0	1	4	6	NM_137001.2	CG4616-RA, transcript variant A (CG4616), mRNA
0	0	1	4	3	NM_134498.2	CG14216-RA (CG14216), mRNA
0	0	1	2	6	NM_132831.1	CG8184-RB (CG8184), mRNA
0	0	1	2	3	NM_165199.1	CG31809-RA (CG31809), mRNA
0	0	1	2	2	NM_140031.1	CG4821-RA, transcript variant A (Tequila), mRNA
0	0	1	2	2	NM_143666.2	CG2009-RA (bip2), mRNA
0	0	0	7	3	NM_079113.2	CG4012-RA (gek), mRNA
0	0	0	6	3	NM_176750.1	CG5613-RB, transcript variant B (CG5613), mRNA
0	0	0	6	3	NM_132987.1	CG5613-RA, transcript variant A (CG5613), mRNA
0	0	0	5	4	NM_141050.1	CG12972-RA (CG12972), mRNA
0	0	0	5	3	NM_166353.1	CG10023-RC, transcript variant C (Fak56D), mRNA
0	0	0	5	3	NM_166352.1	CG10023-RB, transcript variant B (Fak56D), mRNA
0	0	0	5	3	NM_079069.2	CG10023-RA, transcript variant A (Fak56D), mRNA
0	0	0	4	12	NM_080138.2	CG1618-RA (comt), mRNA
0	0	0	4	8	NM_080194.3	CG4195-RA (l(3)73Ah), mRNA
0	0	0	4	7	NM_139549.1	CG11526-RB, transcript variant B (CG11526), mRNA

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