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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12717R-2 
 Symbol CG12717  Full Name CG12717 
 CG No CG12717  Old CG No CG12717 
 Synonyms CG12717 
 Accession No (Link to NCBI) NM_132599.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGGTACGTCGAATTGCCCATA 
 Primer Seq. 3' 
aatctagaggtaccCCCGCAAGGACTTTTCTTGTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGGTACGTCG AATTGCCCAT AATGACTTTA TATTAGTCTC ACGTCAATTG CTGATCAATC 
0061 GAGCCGACAC GGATCCGGAC AAAGGAAGTG CCAGGATGTT GATTATCTAC AGCAGTGGAA 
0121 AGCAGCACAT CGTCACCTTC AAGCTGCCCA ATCTTGCGTG TAGAGTGCAG GATCTGTTTT 
0181 TCCAAATGGA TCTTCAGATC GATGAGAGCA CCACGATTGA TTGCATTGAG AATGCCGGCG 
0241 GTATTATCCA TTTGGTTGTC TCTGTGGGCT TCCCCATCAG TGATAACATT GCCGAGACGG 
0301 TTGCCCATGC GGAGGAGTTC TATAGGAAGG TGCATCAGTC AAAGGCATCA TCGGCTGATG 
0361 CCGCTCCACC CAATGCGATG GCCGGTGAAG AGCACTCCCC TGCGGTTGTC AAACCATCAG 
0421 AGACTGCGGT GGCCAACAAT GGTTGCCAGC CAGCTGCAAG TGCAACCAAA GGCAAGAAGC 
0481 ACAAGAAAAG TCCTTGCGGG  
 in silico PCR Fragment 
0001 CGGTACGTCG AATTGCCCAT AATGACTTTA TATTAGTCTC ACGTCAATTG CTGATCAATC 
0061 GAGCCGACAC GGATCCGGAC AAAGGAAGTG CCAGGATGTT GATTATCTAC AGCAGTGGAA 
0121 AGCAGCACAT CGTCACCTTC AAGCTGCCGA ATCTTGCGTG TAGGGTGCAG GATCTGTTTT 
0181 TCCAAATGGA CCTTCAGATC GATGAGAGCA CCACGATTGA TTGCATTGAG AATGCCGGCG 
0241 GTATTATCCA TTTGGTTGTC TCTGTGGGCT TCCCCATCAG TGATAACATT GCCGAGACGG 
0301 TTGCCCATGC GGAGGAGTTC TATAGGAAGG TGCATCAGTC AAAGGCATCA TCGGCTGATG 
0361 CCGCTCCACC CAATGCGATG GCCGGTGAAG AGCACTCCCC TGCGGTTGTC AAACCATCAG 
0421 AGACTGCGGT GGCCAACAAT GGTTGCCAGC CAGCTGCAAG TGCAACCAAA GGCAAGAAGC 
0481 ACAAGAAAAG TCCTTGCGGG  
 Assemble Data 

12717R-2.IR_full       1   CGGTACGTCGAATTGCCCATAATGACTTTATATTAGTCTCACGTCAATTGCTGATCAATC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     1   CGGTACGTCGAATTGCCCATAATGACTTTATATTAGTCTCACGTCAATTGCTGATCAATC 60


12717R-2.IR_full       61  GAGCCGACACGGATCCGGACAAAGGAAGTGCCAGGATGTTGATTATCTACAGCAGTGGAA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     61  GAGCCGACACGGATCCGGACAAAGGAAGTGCCAGGATGTTGATTATCTACAGCAGTGGAA 120


12717R-2.IR_full       121 AGCAGCACATCGTCACCTTCAAGCTGCCCAATCTTGCGTGTAGAGTGCAGGATCTGTTTT 180
                           |||||||||||||||||||||||||||| |||||||||||||| ||||||||||||||||
12717R-2.in silico     121 AGCAGCACATCGTCACCTTCAAGCTGCCGAATCTTGCGTGTAGGGTGCAGGATCTGTTTT 180


12717R-2.IR_full       181 TCCAAATGGATCTTCAGATCGATGAGAGCACCACGATTGATTGCATTGAGAATGCCGGCG 240
                           |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     181 TCCAAATGGACCTTCAGATCGATGAGAGCACCACGATTGATTGCATTGAGAATGCCGGCG 240


12717R-2.IR_full       241 GTATTATCCATTTGGTTGTCTCTGTGGGCTTCCCCATCAGTGATAACATTGCCGAGACGG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     241 GTATTATCCATTTGGTTGTCTCTGTGGGCTTCCCCATCAGTGATAACATTGCCGAGACGG 300


12717R-2.IR_full       301 TTGCCCATGCGGAGGAGTTCTATAGGAAGGTGCATCAGTCAAAGGCATCATCGGCTGATG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     301 TTGCCCATGCGGAGGAGTTCTATAGGAAGGTGCATCAGTCAAAGGCATCATCGGCTGATG 360


12717R-2.IR_full       361 CCGCTCCACCCAATGCGATGGCCGGTGAAGAGCACTCCCCTGCGGTTGTCAAACCATCAG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     361 CCGCTCCACCCAATGCGATGGCCGGTGAAGAGCACTCCCCTGCGGTTGTCAAACCATCAG 420


12717R-2.IR_full       421 AGACTGCGGTGGCCAACAATGGTTGCCAGCCAGCTGCAAGTGCAACCAAAGGCAAGAAGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12717R-2.in silico     421 AGACTGCGGTGGCCAACAATGGTTGCCAGCCAGCTGCAAGTGCAACCAAAGGCAAGAAGC 480


12717R-2.IR_full       481 ACAAGAAAAGTCCTTGCGGG 500
                           ||||||||||||||||||||
12717R-2.in silico     481 ACAAGAAAAGTCCTTGCGGG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  1  NM_132599.1  CG12717-RA (CG12717), mRNA 
0   0  3  3  3  NM_057791.3  CG17348-RA (drl), mRNA 
0   0  2  3  5  NM_001014622.1  CG33555-RA, transcript variant A (btsz), mRNA 
0   0  2  3  5  NM_001014625.1  CG33555-RB, transcript variant B (btsz), mRNA 
0   0  2  3  4  NM_001014624.1  CG33555-RC, transcript variant C (btsz), mRNA 
0   0  2  3  4  NM_001014623.1  CG33555-RD, transcript variant D (btsz), mRNA 
0   0  2  2  6  NM_001043281.1  CG31151-RC, transcript variant C (wge), mRNA 
0   0  2  2  6  NM_170037.1  CG31151-RA, transcript variant A (wge), mRNA 
0   0  2  2  6  NM_170038.1  CG31151-RB, transcript variant B (wge), mRNA 
0   0  1  5  6  NM_170435.1  CG31009-RA (Cad99C), mRNA 
0   0  1  4  2  NM_140728.2  CG7580-RA (CG7580), mRNA 
0   0  1  3  6  NM_137297.1  CG8303-RA (CG8303), mRNA 
0   0  1  3  4  NM_001043297.1  CG3339-RB, transcript variant B (CG3339), mRNA 
0   0  1  3  4  NM_143300.1  CG3339-RA, transcript variant A (CG3339), mRNA 
0   0  1  3  3  NM_165546.1  CG1358-RC, transcript variant C (CG1358), mRNA 
0   0  1  3  3  NM_136457.2  CG1358-RA, transcript variant A (CG1358), mRNA 
0   0  1  2  2  NM_141401.1  CG2330-RA (CG2330), mRNA 
0   0  0  5  10  NM_079480.2  CG7619-RA (Pros54), mRNA 
0   0  0  5  5  NM_176223.2  CG30115-RD, transcript variant D (CG30115), mRNA 
0   0  0  4  12  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  0  4  8  NM_079937.2  CG1915-RA, transcript variant A (sls), mRNA 
0   0  0  4  7  NM_206290.1  CG33279-RA (CG33279), mRNA 
0   0  0  4  4  NM_079633.2  CG6476-RA, transcript variant A (Su(var)3-9), mRNA 
0   0  0  4  4  NM_140817.1  CG14076-RA (CG14076), mRNA 
0   0  0  4  3  NM_137322.1  CG6472-RA (CG6472), mRNA 
0   0  0  4  3  NM_078681.2  CG32540-RA (CCKLR-17D3), mRNA 
0   0  0  4  2  NM_136004.1  CG15143-RA (CG15143), mRNA 
0   0  0  4  2  NM_058083.2  CG14941-RA (esc), mRNA 
0   0  0  3  8  NM_176248.1  CG30217-RB, transcript variant B (CG30217), mRNA 
0   0  0  3  8  NM_137855.2  CG30217-RA, transcript variant A (CG30217), mRNA 

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