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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12493R-2 
 Symbol CG12493  Full Name CG12493 
 CG No CG12493  Old CG No CG12493 
 Synonyms cg12493, CG12493 
 Accession No (Link to NCBI) NM_139670.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCTGATGACGCTGCCATGGCAA 
 Primer Seq. 3' 
aatctagaggtaccCAACTGTCCAACTGACGACGA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CTGATGACGC TGCCATGNCA ACCAGCGACC CTAAGAAGAA AAAGAAGAAG GTAAAGAAAT 
0061 TGAAACGTTC CCAAAAAGCC AAGGATCGTC TTGTTCGCCC GTTGCCCAAG GCCGTGACCT 
0121 CCAAGGATGC CCTTATGGTT TTGAAAGAGT TAAAGGGCGT GATAATCGAC AACATGCAAA 
0181 TAAAACAAGA CCACGAGGGG AAGAATGTGG CTAACATTGT TGTTAACTCA AAGAAATACG 
0241 ATGCGGAGGG TTCTTCAGAA AACTCTGCAG GGAATGCAGC TTGCGAGAAG GCTCTGCGAG 
0301 AAATCCTAAC CACCAAAATG AAAGCTCTGT TGGCCGAGCC AGNAAAACAG CTCGGGAGGC 
0361 GATGAAGATA ATATACTCGA AAAGATGACC TCTTATGCCG TTTACAAACT TGCTGAGAAA 
0421 TGGAACAgCG ACGCTATTGA TGTTGCAGCC CTGTATAATG ACGTAAAGAA CAAGAAGACG 
0481 TCGTCGTCAG TTGGACAGTT G 
 in silico PCR Fragment 
0001 CTGATGACGC TGCCATGGCA ACCAGCGACC CTAAGAAGAA AAAGAAGAAG GTAAAGAAAT 
0061 TGAAACGTTC CCAAAAAGCC AAGGATCGTC TTGTTCGCCC GTTGCCCAAG GCCGTGACCT 
0121 CCAAGGATGC CCTTATGGTT TTGAAAGAGT TAAAGGGCGT GATAATCGAC AACATGCAAA 
0181 TAAAACAAGA CCACGAGGGG AAGAATGTGG CTAACATTGT TGTTAACTCA AAGAAATACG 
0241 ATGCGGAGGG TTCTTCAGAA AACTCTGCAG GGAATGCAGC TTGCGAGAAG GCTCTGCGAG 
0301 AAATCCTAAC CACCAAAATG AAAGCTCTGT TGGCCGAGCC AG-AAAACAG CTCGGGAGGC 
0361 GATGAAGATA ATATACTCGA AAAGATGACC TCTTATGCCG TTTACAAACT TGCTGAGAAA 
0421 TGGAACAGCG ACGCTATTGA TGTTGCAGCC CTGTATAATG ACGTAAAGAA CAAGAAGACG 
0481 TCGTCGTCAG TTGGACAGTT G 
 Assemble Data 

12493R-2.IR_full       1   CTGATGACGCTGCCATGNCAACCAGCGACCCTAAGAAGAAAAAGAAGAAGGTAAAGAAAT 60
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     1   CTGATGACGCTGCCATGGCAACCAGCGACCCTAAGAAGAAAAAGAAGAAGGTAAAGAAAT 60


12493R-2.IR_full       61  TGAAACGTTCCCAAAAAGCCAAGGATCGTCTTGTTCGCCCGTTGCCCAAGGCCGTGACCT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     61  TGAAACGTTCCCAAAAAGCCAAGGATCGTCTTGTTCGCCCGTTGCCCAAGGCCGTGACCT 120


12493R-2.IR_full       121 CCAAGGATGCCCTTATGGTTTTGAAAGAGTTAAAGGGCGTGATAATCGACAACATGCAAA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     121 CCAAGGATGCCCTTATGGTTTTGAAAGAGTTAAAGGGCGTGATAATCGACAACATGCAAA 180


12493R-2.IR_full       181 TAAAACAAGACCACGAGGGGAAGAATGTGGCTAACATTGTTGTTAACTCAAAGAAATACG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     181 TAAAACAAGACCACGAGGGGAAGAATGTGGCTAACATTGTTGTTAACTCAAAGAAATACG 240


12493R-2.IR_full       241 ATGCGGAGGGTTCTTCAGAAAACTCTGCAGGGAATGCAGCTTGCGAGAAGGCTCTGCGAG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     241 ATGCGGAGGGTTCTTCAGAAAACTCTGCAGGGAATGCAGCTTGCGAGAAGGCTCTGCGAG 300


12493R-2.IR_full       301 AAATCCTAACCACCAAAATGAAAGCTCTGTTGGCCGAGCCAGNAAAACAGCTCGGGAGGC 360
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
12493R-2.in silico     301 AAATCCTAACCACCAAAATGAAAGCTCTGTTGGCCGAGCCAG-AAAACAGCTCGGGAGGC 360


12493R-2.IR_full       361 GATGAAGATAATATACTCGAAAAGATGACCTCTTATGCCGTTTACAAACTTGCTGAGAAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     361 GATGAAGATAATATACTCGAAAAGATGACCTCTTATGCCGTTTACAAACTTGCTGAGAAA 420


12493R-2.IR_full       421 TGGAACAGCGACGCTATTGATGTTGCAGCCCTGTATAATGACGTAAAGAACAAGAAGACG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12493R-2.in silico     421 TGGAACAGCGACGCTATTGATGTTGCAGCCCTGTATAATGACGTAAAGAACAAGAAGACG 480


12493R-2.IR_full       481 TCGTCGTCAGTTGGACAGTTG 501
                           |||||||||||||||||||||
12493R-2.in silico     481 TCGTCGTCAGTTGGACAGTTG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_139670.2  CG12493-RA (CG12493), mRNA 
7.46   36  79  105  94  NM_139709.1  CG10630-RA (CG10630), mRNA 
2.9   14  1  1  1  NM_168102.1  CG32239-RA (Gef64C), mRNA 
0.2   1  3  2  4  NM_141353.1  CG15580-RA, transcript variant A (CG15580), mRNA 
0.2   1  3  2  4  NM_169124.1  CG15580-RB, transcript variant B (CG15580), mRNA 
0.2   1  2  9  9  NM_168387.1  CG6711-RA (Taf2), mRNA 
0   0  5  4  6  NM_079016.3  CG8523-RA (Mdr50), mRNA 
0   0  3  3  5  NM_166259.1  CG30325-RA (CG30325), mRNA 
0   0  3  2  9  NM_137623.2  CG9143-RA (CG9143), mRNA 
0   0  3  2  4  NM_136456.2  CG1360-RA (CG1360), mRNA 
0   0  2  6  18  NM_142783.2  CG13849-RA (Nop56), mRNA 
0   0  2  6  8  NM_139541.1  CG12017-RA (CG12017), mRNA 
0   0  2  5  18  NM_079319.2  CG4153-RA (eIF-2beta), mRNA 
0   0  2  4  12  NM_140709.1  CG13724-RA (CG13724), mRNA 
0   0  2  4  2  NM_134888.2  CG3165-RA (CG3165), mRNA 
0   0  2  3  12  NM_058064.3  CG10206-RA (nop5), mRNA 
0   0  2  3  7  NM_134988.1  CG15432-RA (CG15432), mRNA 
0   0  2  2  7  NM_164375.1  CG18497-RC, transcript variant C (spen), mRNA 
0   0  2  2  7  NM_164374.1  CG18497-RA, transcript variant A (spen), mRNA 
0   0  2  2  7  NM_079979.2  CG18497-RB, transcript variant B (spen), mRNA 
0   0  2  2  3  NM_133003.2  CG32555-RB, transcript variant B (RhoGAPp190), mRNA 
0   0  2  2  3  NM_167573.1  CG32555-RC, transcript variant C (RhoGAPp190), mRNA 
0   0  2  2  3  NM_167572.1  CG32555-RA, transcript variant A (RhoGAPp190), mRNA 
0   0  2  1  2  NM_142388.2  CG18212-RG, transcript variant G (CG18212), mRNA 
0   0  1  12  28  NM_001014547.1  CG3333-RB, transcript variant B (Nop60B), mRNA 
0   0  1  12  28  NM_080381.2  CG3333-RA, transcript variant A (Nop60B), mRNA 
0   0  1  6  11  NM_143281.1  CG6066-RA (CG6066), mRNA 
0   0  1  5  12  NM_137196.1  CG12964-RA (CG12964), mRNA 
0   0  1  5  7  NM_143407.2  CG11305-RA (Sirt7), mRNA 
0   0  1  3  4  NM_132274.2  CG1785-RA (CG1785), mRNA 

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