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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12275R-2 
 Symbol RpS10a  Full Name Ribosomal protein S10a 
 CG No CG12275  Old CG No CG12275 
 Synonyms CG12275, S10, BcDNA:LD32148, RpS10, RpS10a 
 Accession No (Link to NCBI) NM_143319.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		 
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGTTCATTCCAAAAGCCAAT 
 Primer Seq. 3' 
aatctagaggtaccTATTGTCGTAGCGAGAAGCAC 
 Predicted Fragment Size 
490 
 IR fragment full Seq 
0001 ATGTTCATTC CAAAAGCCAA TCGTGTCGCC ATCTACGAGT ACCTCTTCAA GGAGGGTGTG 
0061 CTCGTGGCCA AGAAGGACTC GCCCATCCAG AAGCACCCCG AACTGGATAA GATTCCCAAT 
0121 CTGCAAGTCA TCAAGGTGAT GCAGTCGCTG AACTCACGTG GCTGGGTCAA GGAGCAGTTT 
0181 GCCTGGCGCC ACTTCTACTG GTTGCTGACC AACGAGGGCA TCGAGGAACT GCGCCGCTAT 
0241 CTGCACTTGC CCCCGGAGAT TGTGCCCTCC ACTTTGACGC AAACTACTCG ATCGAACGCA 
0301 GTGCGTCCGC GCGGAGGACC AGGAGGACCC GGAGGAGGAT TCGGAGGCGC CTCCAAAACC 
0361 GATGACGACA GATCGAACTA CAGACGCGGT CCAGGCGCTT ATGGAATGGA CAAGAAAGGC 
0421 GATGTGGGTG CCGGAACCGG ACGGGTCGAA TATCGTGGCG GTTTCGGTCG TGCTTCTCGC 
0481 TACGACAATA  
 in silico PCR Fragment 
0001 ATGTTCATTC CAAAAGCCAA TCGTGTCGCC ATCTACGAGT ACCTCTTCAA GGAGGGTGTG 
0061 CTCGTGGCCA AGAAGGACTC GCCCATCCAG AAGCACTCCG AACTGGATAA GATTCCCAAT 
0121 CTGCAAGTCA TCAAGGTGAT GCAGTCGCTG AACTCACGTG GCTGGGTCAA GGAGCAGTTT 
0181 GCCTGGCGCC ACTTCTACTG GTTGCTGACC AACGAGGGCA TCGAGGAACT GCGCCGCTAT 
0241 CTGCACTTGC CCCCGGAGAT TGTGCCCTCC ACTTTGACGC AAACTACTCG ATCGAACGCA 
0301 GTGCGTCCGC GCGGAGGACC AGGAGGACCC GGAGGAGGAT TCGGAGGCGC CTCCAAAACC 
0361 GATGACGACA GATCGAACTA CAGACGCGGT CCAGGCGCAT ATGGAATGGA CAAGAAAGGC 
0421 GATGTGGGTG CCGGAACCGG ACGGGTCGAA TATCGTGGCG GTTTCGGTCG TGCTTCTCGC 
0481 TACGACAATT  
 Assemble Data 

12275R-2.IR_full       1   ATGTTCATTCCAAAAGCCAATCGTGTCGCCATCTACGAGTACCTCTTCAAGGAGGGTGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     1   ATGTTCATTCCAAAAGCCAATCGTGTCGCCATCTACGAGTACCTCTTCAAGGAGGGTGTG 60


12275R-2.IR_full       61  CTCGTGGCCAAGAAGGACTCGCCCATCCAGAAGCACCCCGAACTGGATAAGATTCCCAAT 120
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
12275R-2.in silico     61  CTCGTGGCCAAGAAGGACTCGCCCATCCAGAAGCACTCCGAACTGGATAAGATTCCCAAT 120


12275R-2.IR_full       121 CTGCAAGTCATCAAGGTGATGCAGTCGCTGAACTCACGTGGCTGGGTCAAGGAGCAGTTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     121 CTGCAAGTCATCAAGGTGATGCAGTCGCTGAACTCACGTGGCTGGGTCAAGGAGCAGTTT 180


12275R-2.IR_full       181 GCCTGGCGCCACTTCTACTGGTTGCTGACCAACGAGGGCATCGAGGAACTGCGCCGCTAT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     181 GCCTGGCGCCACTTCTACTGGTTGCTGACCAACGAGGGCATCGAGGAACTGCGCCGCTAT 240


12275R-2.IR_full       241 CTGCACTTGCCCCCGGAGATTGTGCCCTCCACTTTGACGCAAACTACTCGATCGAACGCA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     241 CTGCACTTGCCCCCGGAGATTGTGCCCTCCACTTTGACGCAAACTACTCGATCGAACGCA 300


12275R-2.IR_full       301 GTGCGTCCGCGCGGAGGACCAGGAGGACCCGGAGGAGGATTCGGAGGCGCCTCCAAAACC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     301 GTGCGTCCGCGCGGAGGACCAGGAGGACCCGGAGGAGGATTCGGAGGCGCCTCCAAAACC 360


12275R-2.IR_full       361 GATGACGACAGATCGAACTACAGACGCGGTCCAGGCGCTTATGGAATGGACAAGAAAGGC 420
                           |||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
12275R-2.in silico     361 GATGACGACAGATCGAACTACAGACGCGGTCCAGGCGCATATGGAATGGACAAGAAAGGC 420


12275R-2.IR_full       421 GATGTGGGTGCCGGAACCGGACGGGTCGAATATCGTGGCGGTTTCGGTCGTGCTTCTCGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12275R-2.in silico     421 GATGTGGGTGCCGGAACCGGACGGGTCGAATATCGTGGCGGTTTCGGTCGTGCTTCTCGC 480


12275R-2.IR_full       481 TACGACAATA 490
                           ||||||||| 
12275R-2.in silico     481 TACGACAATT 490



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   472  0  0  8  NM_143319.2  CG12275-RA (RpS10a), mRNA 
1.69   8  33  60  52  NM_167668.1  CG14206-RB, transcript variant B (RpS10b), mRNA 
1.69   8  33  60  52  NM_134480.2  CG14206-RC, transcript variant C (RpS10b), mRNA 
0.21   1  3  2  5  NM_001038965.1  CG9924-RE, transcript variant E (CG9924), mRNA 
0   0  8  13  25  NM_135592.2  CG7299-RA (CG7299), mRNA 
0   0  5  38  59  NM_078712.2  CG12794-RA (lcs), mRNA 
0   0  3  5  15  NM_141224.1  CG12586-RA (CG12586), mRNA 
0   0  3  4  3  NM_142313.2  CG10326-RA (CG10326), mRNA 
0   0  2  4  33  NM_136816.1  CG13214-RA, transcript variant A (CG13214), mRNA 
0   0  2  3  3  NM_176098.1  CG33134-RA (debcl), mRNA 
0   0  2  3  3  NM_140759.2  CG5546-RA (MED19), mRNA 
0   0  2  3  2  NM_143086.2  CG13651-RA (danr), mRNA 
0   0  2  2  2  NM_169873.1  CG7535-RB, transcript variant B (GluClalpha), mRNA 
0   0  2  2  2  NM_142570.2  CG7535-RA, transcript variant A (GluClalpha), mRNA 
0   0  2  2  2  NM_001014641.1  CG7535-RC, transcript variant C (GluClalpha), mRNA 
0   0  2  2  2  NM_001038972.1  CG7535-RD, transcript variant D (GluClalpha), mRNA 
0   0  1  3  16  NM_001032407.1  CG33956-RA, transcript variant A (kay), mRNA 
0   0  1  3  3  NM_137257.1  CG7786-RA (CG7786), mRNA 
0   0  1  3  3  NM_170518.2  CG12066-RB, transcript variant B (Pka-C2), mRNA 
0   0  1  3  3  NM_079856.2  CG12066-RA, transcript variant A (Pka-C2), mRNA 
0   0  1  2  6  NM_135248.1  CG13776-RA (CG13776), mRNA 
0   0  1  2  5  NM_137792.1  CG6437-RA (GlcT-1), mRNA 
0   0  0  8  15  NM_136997.1  CG15870-RA (CG15870), mRNA 
0   0  0  8  13  NM_079355.2  CG6988-RA, transcript variant A (Pdi), mRNA 
0   0  0  8  8  NM_168322.1  CG10537-RB, transcript variant B (Rdl), mRNA 
0   0  0  8  8  NM_079267.2  CG10537-RA, transcript variant A (Rdl), mRNA 
0   0  0  8  8  NM_168321.1  CG10537-RC, transcript variant C (Rdl), mRNA 
0   0  0  7  15  NM_143742.2  CG5812-RA (GCR(ich)), mRNA 
0   0  0  7  10  NM_168614.2  CG6988-RD, transcript variant D (Pdi), mRNA 
0   0  0  6  4  NM_205880.1  CG2674-RE, transcript variant E (M(2)21AB), mRNA 

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