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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 12231R-1 
 Symbol CG12231  Full Name CG12231 
 CG No CG12231  Old CG No CG12231 
 Synonyms CG12231 
 Accession No (Link to NCBI) NM_133162.1 
 Inserted Chr. X 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAGGGAGATCAGTACCGAAGAC 
 Primer Seq. 3' 
aatctagaggtaccTGAAATTGACGGAAGTGAGGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AGGGAGATCA GTACCGAAGA CATTGAACAG AGGGAGCCGG TAATGCTGAA CATCTACGAC 
0061 CTGTCCACCA GCAACAACTA CACTTTTCCG CTGGGCTTGG GTGTCTTCCA TTCCGGCGTC 
0121 CAGTTGTACG GGCGAGAGTA CGCATTTTTG GCGCTCAATC TCTCCATTTC CGGCATCTTT 
0181 GAGATTCATC CCTGCAATGG GCAGGAGGAG TTGGGCGAAC ACTTCCGTTT CCGGAAGAGC 
0241 ATTCTGCTCG GCTACACCGA CTTTACCTGC GCTGAAGTGA AGCGCGTTAT CAACCTGCTG 
0301 GGCTTCGAGT TCCGCGGGAC CAGCTACCAC TTGACCAGCA AGAATTGCAA CCACTTCTCC 
0361 AACTGCCTGG CGCACCTCGT CTGTGGCCGT AAAATTCCGC GTTGGGTCAA CCGCCTGGCC 
0421 TACCTCATAA CGTGTGTCCC ATTCCTCGAA CGCTTTGTGG TCTCCAGTCC ATCACATTAC 
0481 CCTCACTTCC GTCAATTTCA  
 in silico PCR Fragment 
0001 AGGGAGATCA GTACCGAAGA CATTGAACAG AGGGAGCCGG TAATGCTGAA CATCTACGAC 
0061 CTGTCCACCA GCAACAACTA CACTTTTCCG CTGGGCTTGG GTGTCTTCCA TTCCGGCGTC 
0121 CAGTTGTACG GGCGAGAGTA CGCATTTTTG GCGCTCAATC TCTCCATTTC CGGCATCTTT 
0181 GAGATTCATC CCTGCAATGG GCAGGAGGAG TTGGGCGAAC ACTTCCGTTT CCGGAAGAGC 
0241 ATTCTGCTCG GCTACACCGA CTTTACCTGC GCTGAAGTGA AGCGCGTTAT CAACCTGCTG 
0301 GGCTTCGAGT TCCGCGGGAC CAGCTACCAC TTGACCAGCA AGAATTGCAA CCACTTCTCC 
0361 AACTGCCTGG CGCATCTCGT CTGTGGCCGT AAAATTCCGC GTTGGGTCAA CCGCCTGGCC 
0421 TACCTCATAA CGTGTGTCCC ATTCCTCGAA CGCTTTGTGG TCTCCAGTCC ATCACATTAC 
0481 CCTCACTTCC GTCAATTTCA  
 Assemble Data 

12231R-1.IR_full       1   AGGGAGATCAGTACCGAAGACATTGAACAGAGGGAGCCGGTAATGCTGAACATCTACGAC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     1   AGGGAGATCAGTACCGAAGACATTGAACAGAGGGAGCCGGTAATGCTGAACATCTACGAC 60


12231R-1.IR_full       61  CTGTCCACCAGCAACAACTACACTTTTCCGCTGGGCTTGGGTGTCTTCCATTCCGGCGTC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     61  CTGTCCACCAGCAACAACTACACTTTTCCGCTGGGCTTGGGTGTCTTCCATTCCGGCGTC 120


12231R-1.IR_full       121 CAGTTGTACGGGCGAGAGTACGCATTTTTGGCGCTCAATCTCTCCATTTCCGGCATCTTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     121 CAGTTGTACGGGCGAGAGTACGCATTTTTGGCGCTCAATCTCTCCATTTCCGGCATCTTT 180


12231R-1.IR_full       181 GAGATTCATCCCTGCAATGGGCAGGAGGAGTTGGGCGAACACTTCCGTTTCCGGAAGAGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     181 GAGATTCATCCCTGCAATGGGCAGGAGGAGTTGGGCGAACACTTCCGTTTCCGGAAGAGC 240


12231R-1.IR_full       241 ATTCTGCTCGGCTACACCGACTTTACCTGCGCTGAAGTGAAGCGCGTTATCAACCTGCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     241 ATTCTGCTCGGCTACACCGACTTTACCTGCGCTGAAGTGAAGCGCGTTATCAACCTGCTG 300


12231R-1.IR_full       301 GGCTTCGAGTTCCGCGGGACCAGCTACCACTTGACCAGCAAGAATTGCAACCACTTCTCC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     301 GGCTTCGAGTTCCGCGGGACCAGCTACCACTTGACCAGCAAGAATTGCAACCACTTCTCC 360


12231R-1.IR_full       361 AACTGCCTGGCGCACCTCGTCTGTGGCCGTAAAATTCCGCGTTGGGTCAACCGCCTGGCC 420
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     361 AACTGCCTGGCGCATCTCGTCTGTGGCCGTAAAATTCCGCGTTGGGTCAACCGCCTGGCC 420


12231R-1.IR_full       421 TACCTCATAACGTGTGTCCCATTCCTCGAACGCTTTGTGGTCTCCAGTCCATCACATTAC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
12231R-1.in silico     421 TACCTCATAACGTGTGTCCCATTCCTCGAACGCTTTGTGGTCTCCAGTCCATCACATTAC 480


12231R-1.IR_full       481 CCTCACTTCCGTCAATTTCA 500
                           ||||||||||||||||||||
12231R-1.in silico     481 CCTCACTTCCGTCAATTTCA 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_133162.1  CG12231-RA (CG12231), mRNA 
0   0  4  8  16  NM_136769.2  CG7222-RA (CG7222), mRNA 
0   0  4  2  3  NM_057439.2  CG2759-RA (w), mRNA 
0   0  2  4  6  NM_078934.2  CG8714-RA (sut1), mRNA 
0   0  2  3  4  NM_134793.2  CG7291-RA (NPC2), mRNA 
0   0  2  2  2  NM_176692.1  CG3960-RD, transcript variant D (CG3960), mRNA 
0   0  1  4  5  NM_169450.1  CG14741-RA (CG14741), mRNA 
0   0  1  3  4  NM_167599.1  CG7536-RB, transcript variant B (CG7536), mRNA 
0   0  1  3  4  NM_133037.2  CG7536-RA, transcript variant A (CG7536), mRNA 
0   0  1  3  4  NM_166200.2  CG8912-RA, transcript variant A (Psi), mRNA 
0   0  1  3  4  NM_057775.3  CG8912-RB, transcript variant B (Psi), mRNA 
0   0  1  3  4  NM_206140.1  CG8912-RC, transcript variant C (Psi), mRNA 
0   0  1  3  2  NM_165352.2  CG9339-RC, transcript variant C (CG9339), mRNA 
0   0  1  3  2  NM_165351.2  CG9339-RF, transcript variant F (CG9339), mRNA 
0   0  1  3  2  NM_165350.2  CG9339-RB, transcript variant B (CG9339), mRNA 
0   0  1  3  2  NM_165348.2  CG9339-RE, transcript variant E (CG9339), mRNA 
0   0  1  3  2  NM_136229.3  CG9339-RA, transcript variant A (CG9339), mRNA 
0   0  1  3  2  NM_165349.2  CG9339-RG, transcript variant G (CG9339), mRNA 
0   0  1  3  2  NM_206016.1  CG9339-RH, transcript variant H (CG9339), mRNA 
0   0  1  3  2  NM_206017.1  CG9339-RD, transcript variant D (CG9339), mRNA 
0   0  1  2  13  NM_080341.2  CG12737-RA, transcript variant A (Crag), mRNA 
0   0  1  2  13  NM_167164.2  CG12737-RB, transcript variant B (Crag), mRNA 
0   0  1  2  5  NM_168898.1  CG32436-RA (CG32436), mRNA 
0   0  1  2  5  NM_139802.2  CG32392-RB, transcript variant B (CG32392), mRNA 
0   0  1  2  5  NM_132507.1  CG2247-RA (CG2247), mRNA 
0   0  1  2  4  NM_057741.3  CG4720-RA, transcript variant A (Pk92B), mRNA 
0   0  1  2  4  NM_206518.1  CG4720-RB, transcript variant B (Pk92B), mRNA 
0   0  1  2  3  NM_142869.3  CG4656-RA (CG4656), mRNA 
0   0  1  2  2  NM_137530.1  CG15080-RA (CG15080), mRNA 
0   0  1  2  2  NM_132132.1  CG4557-RA (CG4557), mRNA 

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