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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10734R-3 
 Symbol CG10734  Full Name CG10734 
 CG No CG10734  Old CG No CG10734 
 Synonyms CG10734 
 Accession No (Link to NCBI) NM_137246.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
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 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgAACGCAAATCGCGAAGGCAAA 
 Primer Seq. 3' 
aatctagaggtaccCGCACCTTGTTGTATAACCAC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 AACGCAAATC GCGAAGGCAA ACAGCTAAGA ATACTACTGA TAAGGGGCTA ATGGATTGTG 
0061 ACGTAAATAT GGAACCTTCA CGATATGTGC CGCCCATTAC AAATCCAATG GAAAGGATCA 
0121 GCGAAGCCGG GACTAAAAAT CAACCGCATA AGTTGGAAAC TGAGAAACCA CGACGACTGC 
0181 GCGGAAGGCC GAAAAATATT GTGAAATCCT CTAGTCTTAT TGCTTCAGAC CATCGCAAGG 
0241 AAAATCCTAT GGAAATAAAC GATGAACAGA AGACTTTCAC AAAGATATTA AAGAAAGTAA 
0301 AGCTCAAGGT CGACGATTAT TTGAAGGACA TGAAGCTGGC TGCTGCTTTT CGCAAGCAAC 
0361 AGAAAAAGTC GCAGCTACAA GTTAGTCGCC GAAAATTAGG ATTAGCTAAG CAGCGTCAAC 
0421 TAGGACGATC CAAAAAGGAT TCATCAAAAG TGAAGGAGAG ACAAATGGAT GAGCAATCAG 
0481 TGGTTATACA ACAAGGTGCG  
 in silico PCR Fragment 
0001 AACGCAAATC GCGAAGGCAA ACAGCTAAGA ATACTACTGA TAAGGGGCTA ATGGATTGTG 
0061 ACGTAAATAT GGAACCTTCA CGATATGTGC CGCCCATTAC AAATCCAATG GAAAGGATCA 
0121 GCGAAGCCGG GACTAAAAAT CAACCGCATA AGTTGGAAAC TGAGAAACCA CGACGACTGC 
0181 GCGGAAGGCC GAAAAATATT GTGAAATCCT CTAGTCTTAT TGCTTCAGAC CATCGCAAGG 
0241 AAAATCCTAT GGAAATAAAC GATGAACAGA AGACTTTCAC AAAGATATTA AAGAAAGTAA 
0301 AGCTCAAGGT CGACGATTAT TTGAAGGACA TGAAGCTGGC TGCTGCTTTT CGCAAGCAAC 
0361 AGAAAAAGTC GCAGCTACAA GTTAGTCGCC GAAAATTAGG ATTAGCTAAG CAGCGTCAAC 
0421 TAGGACGATC CAAAAAGGAT TCATCAAAAG TGAAGGAGAG ACAAATGGAT GAGCAATCAG 
0481 TGGTTATACA ACAAGGTGCG  
 Assemble Data 

10734R-3.IR_full       1   AACGCAAATCGCGAAGGCAAACAGCTAAGAATACTACTGATAAGGGGCTAATGGATTGTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     1   AACGCAAATCGCGAAGGCAAACAGCTAAGAATACTACTGATAAGGGGCTAATGGATTGTG 60


10734R-3.IR_full       61  ACGTAAATATGGAACCTTCACGATATGTGCCGCCCATTACAAATCCAATGGAAAGGATCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     61  ACGTAAATATGGAACCTTCACGATATGTGCCGCCCATTACAAATCCAATGGAAAGGATCA 120


10734R-3.IR_full       121 GCGAAGCCGGGACTAAAAATCAACCGCATAAGTTGGAAACTGAGAAACCACGACGACTGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     121 GCGAAGCCGGGACTAAAAATCAACCGCATAAGTTGGAAACTGAGAAACCACGACGACTGC 180


10734R-3.IR_full       181 GCGGAAGGCCGAAAAATATTGTGAAATCCTCTAGTCTTATTGCTTCAGACCATCGCAAGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     181 GCGGAAGGCCGAAAAATATTGTGAAATCCTCTAGTCTTATTGCTTCAGACCATCGCAAGG 240


10734R-3.IR_full       241 AAAATCCTATGGAAATAAACGATGAACAGAAGACTTTCACAAAGATATTAAAGAAAGTAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     241 AAAATCCTATGGAAATAAACGATGAACAGAAGACTTTCACAAAGATATTAAAGAAAGTAA 300


10734R-3.IR_full       301 AGCTCAAGGTCGACGATTATTTGAAGGACATGAAGCTGGCTGCTGCTTTTCGCAAGCAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     301 AGCTCAAGGTCGACGATTATTTGAAGGACATGAAGCTGGCTGCTGCTTTTCGCAAGCAAC 360


10734R-3.IR_full       361 AGAAAAAGTCGCAGCTACAAGTTAGTCGCCGAAAATTAGGATTAGCTAAGCAGCGTCAAC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     361 AGAAAAAGTCGCAGCTACAAGTTAGTCGCCGAAAATTAGGATTAGCTAAGCAGCGTCAAC 420


10734R-3.IR_full       421 TAGGACGATCCAAAAAGGATTCATCAAAAGTGAAGGAGAGACAAATGGATGAGCAATCAG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10734R-3.in silico     421 TAGGACGATCCAAAAAGGATTCATCAAAAGTGAAGGAGAGACAAATGGATGAGCAATCAG 480


10734R-3.IR_full       481 TGGTTATACAACAAGGTGCG 500
                           ||||||||||||||||||||
10734R-3.in silico     481 TGGTTATACAACAAGGTGCG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137246.1  CG10734-RA (CG10734), mRNA 
0   0  2  2  3  NM_165190.1  CG17927-RK, transcript variant K (Mhc), mRNA 
0   0  2  2  2  NM_165181.1  CG17927-RC, transcript variant C (Mhc), mRNA 
0   0  2  2  2  NM_165182.1  CG17927-RG, transcript variant G (Mhc), mRNA 
0   0  2  2  2  NM_165183.1  CG17927-RE, transcript variant E (Mhc), mRNA 
0   0  2  2  2  NM_165184.1  CG17927-RJ, transcript variant J (Mhc), mRNA 
0   0  2  2  2  NM_165185.1  CG17927-RF, transcript variant F (Mhc), mRNA 
0   0  2  2  2  NM_165186.1  CG17927-RD, transcript variant D (Mhc), mRNA 
0   0  2  2  2  NM_165187.1  CG17927-RA, transcript variant A (Mhc), mRNA 
0   0  2  2  2  NM_165188.1  CG17927-RI, transcript variant I (Mhc), mRNA 
0   0  2  2  2  NM_078863.4  CG17927-RH, transcript variant H (Mhc), mRNA 
0   0  2  2  2  NM_165191.1  CG17927-RL, transcript variant L (Mhc), mRNA 
0   0  2  2  2  NM_165192.1  CG17927-RM, transcript variant M (Mhc), mRNA 
0   0  2  2  2  NM_165189.1  CG17927-RB, transcript variant B (Mhc), mRNA 
0   0  1  4  2  NM_168402.1  CG32055-RA (CG32055), mRNA 
0   0  1  2  3  NM_168116.1  CG10645-RC, transcript variant C (lama), mRNA 
0   0  1  2  3  NM_079209.2  CG10645-RB, transcript variant B (lama), mRNA 
0   0  1  2  3  NM_168115.1  CG10645-RA, transcript variant A (lama), mRNA 
0   0  1  2  2  NM_001043084.1  CG34123-RD, transcript variant D (CG34123), mRNA 
0   0  1  2  2  NM_001043083.1  CG34123-RB, transcript variant B (CG34123), mRNA 
0   0  1  2  2  NM_001043085.1  CG34123-RC, transcript variant C (CG34123), mRNA 
0   0  1  2  2  NM_131982.1  CG3323-RA (CG3323), mRNA 
0   0  0  6  6  NM_176144.1  CG33144-RA (CG33144), mRNA 
0   0  0  6  4  NM_136051.1  CG10336-RB, transcript variant B (CG10336), mRNA 
0   0  0  6  4  NM_165261.1  CG10336-RA, transcript variant A (CG10336), mRNA 
0   0  0  4  15  NM_144448.2  CG1915-RC, transcript variant C (sls), mRNA 
0   0  0  4  5  NM_079472.2  CG9390-RB, transcript variant B (AcCoAS), mRNA 
0   0  0  4  5  NM_168894.1  CG9390-RA, transcript variant A (AcCoAS), mRNA 
0   0  0  4  5  NM_001014599.1  CG9390-RC, transcript variant C (AcCoAS), mRNA 
0   0  0  4  4  NM_135520.2  CG4946-RA (CG4946), mRNA 

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