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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10232R-4 |
| Symbol |
CG10232 |
Full Name |
CG10232 |
| CG No |
CG10232 |
Old CG No |
CG10232 |
| Synonyms |
SP14, CG10232 |
| Accession No (Link to NCBI) |
NM_142918.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCTACCAACGTCCTGTGGACAA |
| Primer Seq. 3' |
aatctagaggtaccCTGTTTTTCGTGTAGCCCCAA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CTACCAACGT CCTGTGGACA AGCTCCGCCT CTTTATCGAA TGGCATATGG CACAGCGGCG
0061 AGGcCAAATG AATATCCTTG GATGGCCATG CTTATCTACG AGAATCGCAG ACTATCGACC
0121 ATGACCAACA ACTGTAGTGG CTCCCTGATT AACAAACGCT ACGTCCTCAC CGCTGCACAC
0181 TGTGTGGTCA AGGATAAAAT GGTGAACACA GATTTGGTGC TCAGGAGAGT GCGGCTGGGC
0241 GAGCATGATA TTACCACCAA TCCGGATTGC GACTTTACAG GAAACTGCGC TGCCCCCTTT
0301 GTAGAGATCG GCATCGAGTA TTTCAATGTC CACGAACAAT ATTTCAACAC CTCAAGGTTT
0361 GAAAGCGATA TCGCCTTGGT GCGTCTTCAA ACGCCAGTTC GATATACCCA CGAAATTCTA
0421 CCGATTTGTG TGCCGAAAGA TCCCATNCCC CTTGCATAAT CACCCATTGC AAATCGCCGG
0481 TTGGGGCTAC ACGAAAAACA G |
| in silico PCR Fragment |
0001 CTACCAACGT CCTGTGGACA AGCTCCGCCT CTTTATCGAA TGGCATATGG CACAGCGGCG
0061 AGGCCAAATG AATATCCTTG GATGGCCATG CTTATCTACG AGAATCGCAG ACTATCGACC
0121 ATGACCAACA ACTGTAGTGG CTCCCTGATT AACAAACGCT ACGTCCTCAC CGCTGCACAC
0181 TGTGTGGTCA AGGATAAAAT GGTGAACACA GATTTGGTGC TCAGGAGAGT GCGGCTGGGC
0241 GAGCATGATA TTACCACCAA TCCGGATTGC GACTTTACAG GAAACTGCGC TGCCCCCTTT
0301 GTAGAGATCG GCATCGAGTA TTTCAATGTC CACGAACAAT ATTTCAACAC CTCAAGGTTT
0361 GAAAGCGATA TCGCCTTGGT GCGTCTTCAA ACGCCAGTTC GATATACCCA CGAAATTCTA
0421 CCGATTTGTG TGCCGAAAGA TCCCAT-CCC CTTGCATAAT CACCCATTGC AAATCGCCGG
0481 TTGGGGCTAC ACGAAAAACA G |
| Assemble Data |
10232R-4.IR_full 1 CTACCAACGTCCTGTGGACAAGCTCCGCCTCTTTATCGAATGGCATATGGCACAGCGGCG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 1 CTACCAACGTCCTGTGGACAAGCTCCGCCTCTTTATCGAATGGCATATGGCACAGCGGCG 60
10232R-4.IR_full 61 AGGCCAAATGAATATCCTTGGATGGCCATGCTTATCTACGAGAATCGCAGACTATCGACC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 61 AGGCCAAATGAATATCCTTGGATGGCCATGCTTATCTACGAGAATCGCAGACTATCGACC 120
10232R-4.IR_full 121 ATGACCAACAACTGTAGTGGCTCCCTGATTAACAAACGCTACGTCCTCACCGCTGCACAC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 121 ATGACCAACAACTGTAGTGGCTCCCTGATTAACAAACGCTACGTCCTCACCGCTGCACAC 180
10232R-4.IR_full 181 TGTGTGGTCAAGGATAAAATGGTGAACACAGATTTGGTGCTCAGGAGAGTGCGGCTGGGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 181 TGTGTGGTCAAGGATAAAATGGTGAACACAGATTTGGTGCTCAGGAGAGTGCGGCTGGGC 240
10232R-4.IR_full 241 GAGCATGATATTACCACCAATCCGGATTGCGACTTTACAGGAAACTGCGCTGCCCCCTTT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 241 GAGCATGATATTACCACCAATCCGGATTGCGACTTTACAGGAAACTGCGCTGCCCCCTTT 300
10232R-4.IR_full 301 GTAGAGATCGGCATCGAGTATTTCAATGTCCACGAACAATATTTCAACACCTCAAGGTTT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 301 GTAGAGATCGGCATCGAGTATTTCAATGTCCACGAACAATATTTCAACACCTCAAGGTTT 360
10232R-4.IR_full 361 GAAAGCGATATCGCCTTGGTGCGTCTTCAAACGCCAGTTCGATATACCCACGAAATTCTA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10232R-4.in silico 361 GAAAGCGATATCGCCTTGGTGCGTCTTCAAACGCCAGTTCGATATACCCACGAAATTCTA 420
10232R-4.IR_full 421 CCGATTTGTGTGCCGAAAGATCCCATNCCCCTTGCATAATCACCCATTGCAAATCGCCGG 480
|||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||
10232R-4.in silico 421 CCGATTTGTGTGCCGAAAGATCCCAT-CCCCTTGCATAATCACCCATTGCAAATCGCCGG 480
10232R-4.IR_full 481 TTGGGGCTACACGAAAAACAG 501
|||||||||||||||||||||
10232R-4.in silico 481 TTGGGGCTACACGAAAAACAG 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_142918.1 |
CG10232-RA (CG10232), mRNA |
| 0 |
0 |
5 |
13 |
30 |
NM_169868.1 |
CG31220-RA (CG31220), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_142974.2 |
CG17786-RA (CG17786), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_136696.1 |
CG12133-RA (CG12133), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_136518.3 |
CG2183-RA (CG2183), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_143287.1 |
CG5909-RA (CG5909), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_170199.1 |
CG11836-RB, transcript variant B (CG11836), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143076.2 |
CG11836-RA, transcript variant A (CG11836), mRNA |
| 0 |
0 |
1 |
8 |
3 |
NM_142911.2 |
CG16705-RA (CG16705), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079614.2 |
CG7996-RA (snk), mRNA |
| 0 |
0 |
0 |
3 |
29 |
NM_169869.1 |
CG31219-RA (CG31219), mRNA |
| 0 |
0 |
0 |
3 |
19 |
NM_169517.1 |
CG8870-RA (CG8870), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_144448.2 |
CG1915-RC, transcript variant C (sls), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_001032224.1 |
CG1865-RB, transcript variant B (Spn43Ab), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_080065.3 |
CG1865-RA, transcript variant A (Spn43Ab), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_141909.2 |
CG12256-RA (CG12256), mRNA |
| 0 |
0 |
0 |
3 |
5 |
NM_167738.1 |
CG32523-RA (CG32523), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_057349.4 |
CG31349-RB, transcript variant B (pyd), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_169246.2 |
CG31349-RC, transcript variant C (pyd), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_169245.2 |
CG31349-RA, transcript variant A (pyd), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_139755.2 |
CG6467-RA (Jon65Aiv), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_001014485.1 |
CG33515-RA (CG33515), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_139466.3 |
CG16761-RA (Cyp4d20), mRNA |
| 0 |
0 |
0 |
2 |
17 |
NM_144395.3 |
CG18754-RA (CG18754), mRNA |
| 0 |
0 |
0 |
2 |
13 |
NM_143527.2 |
CG9733-RA (CG9733), mRNA |
| 0 |
0 |
0 |
2 |
8 |
NM_136591.3 |
CG8213-RA (CG8213), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_057511.3 |
CG3936-RA (N), mRNA |
| 0 |
0 |
0 |
2 |
6 |
NM_141193.3 |
CG1102-RA (MP1), mRNA |
| 0 |
0 |
0 |
2 |
5 |
NM_140114.1 |
CG14164-RA (CG14164), mRNA |
| 0 |
0 |
0 |
2 |
4 |
NM_001038785.1 |
CG31919-RE, transcript variant E (CG31919), mRNA |
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| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| + |
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| DB |
 |
| + |
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| DC |
 |
| + |
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| DM |
 |
| + |
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| VM |
 |
| + |
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| VB |
 |
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