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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
4427R-1 |
| Symbol |
cbt |
Full Name |
cabut |
| CG No |
CG4427 |
Old CG No |
CG4427 |
| Synonyms |
CG4427, EP2237, EP(2)2237, dTIEG, cbt |
| Accession No (Link to NCBI) |
NM_164384.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Bartok O, Teesalu M, Ashwall-Fluss R, Pandey V, Hanan M, Rovenko BM, Poukkula M, Havula E, Moussaieff A, Vodala S, Nahmias Y, Kadener S, Hietakangas V.
The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing.
EMBO J (2015) 34(11) 1538-53
[
PubMed ID = 25916830
]
[
RRC reference
]
Bauke AC, Sasse S, Matzat T, Klämbt C.
A transcriptional network controlling glial development in the Drosophila visual system.
Development (2015) 142(12) 2184-93
[
PubMed ID = 26015542
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Havula E, Teesalu M, Hyötyläinen T, Seppälä H, Hasygar K, Auvinen P, Orešič M, Sandmann T, Hietakangas V.
Mondo/ChREBP-Mlx-regulated transcriptional network is essential for dietary sugar tolerance in Drosophila.
PLoS Genet (2013) 9(4) e1003438
[
PubMed ID = 23593032
]
[
RRC reference
]
Rodriguez I.
Drosophila TIEG is a modulator of different signalling pathways involved in wing patterning and cell proliferation.
PLoS One (2011) 6(4) e18418
[
PubMed ID = 21494610
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATACCTTGCTACCTTCACCAC |
| Primer Seq. 3' |
aatctagaggtaccTTCTGCAGCACGCTTTCTGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATACCTTGCT ACCTTCACCA CCCGCAACgN CCCCACTAAG GGAAAACAAG TTGGAAATCG
0061 TCGCCAAGGA CGAACAACAG GTGAACGAGA ATCTGCTAAA GGCAAAGCTC AANGCTGGTG
0121 GCCCAAAAGA GTCAGAAAAA ATGGAGGGAT TATTACACCA AATCCATCGG ACACGGGAAG
0181 ACGAGGCTCC CCGAAATNAG CGGTTCCCAA CAAGAAACCC CGTTTGGAAC AACTTGCTAT
0241 GAGCATGACA CCGCCGCCCG GATCAAAAGC TGGACGATGA TCAGAAAGCG GAAAGGGTTA
0301 GCGTCATCAT GCGGGTCAAC AGTTCTGGCG CTGTCTCTTC TAGTAGCCAA GACGAGAACT
0361 CATCTAGTTC CACCTCCTGC TGTAGTTCCT CTTCCAACAC AAACACAAGT ACAAGTTCAG
0421 TACCACCCAC TGTGGAGGAC GACTATCCAG AGGCCAATGT GTGGCGCAAT CTCAAGTTCA
0481 AAATGAACAG AAAGCGTGCT GCAGAA |
| in silico PCR Fragment |
0001 ATACCTTGCT ACCTTCACCA CCCGCAACGC CCCCACTAAG GGAAAACAAG TTGGAAATCG
0061 TCGCCAAGGA CGAACAACAG GTGAACGAGA ATCTGCTAAA GGCAAAGCTC AA-GCTGGTG
0121 GCCCAAAAGA GTCAG-AAAA ATGGAGGGAT TATTACACCA AATCCATCGG ACAC-GGAAG
0181 ACGAGGCT-C CCGAAAT-AG CGGTTCCCAA CAAGAAACCC CGTTTGGAAC AACCTGCTAT
0241 GAGCATGACA CCGCCG-CCG GATCAAAAGC TGGACGATGA TCAGAAAGCG GAAAGGGTTA
0301 GCGTCATCAT GCGGGTCAAC AGTTCTGGCG CTGTCTCTTC TAGTAGCCAA GACGAGAACT
0361 CATCTAGTTC CACCTCCTGC TGTAGTTCCT CTTCCAACAC AAACACAAGT ACAAGTTCAG
0421 TACCACCCAC TGTGGAGGAC GACTATCCAG AGGCCAATGT GTGGCGCAAT CTCAAGTTCA
0481 AAATGAACAG AAAGCGTGCT GCAGAA |
| Assemble Data |
4427R-1.IR_full 1 ATACCTTGCTACCTTCACCACCCGCAACGNCCCCACTAAGGGAAAACAAGTTGGAAATCG 60
||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
4427R-1.in silico 1 ATACCTTGCTACCTTCACCACCCGCAACGCCCCCACTAAGGGAAAACAAGTTGGAAATCG 60
4427R-1.IR_full 61 TCGCCAAGGACGAACAACAGGTGAACGAGAATCTGCTAAAGGCAAAGCTCAANGCTGGTG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
4427R-1.in silico 61 TCGCCAAGGACGAACAACAGGTGAACGAGAATCTGCTAAAGGCAAAGCTCAA-GCTGGTG 120
4427R-1.IR_full 121 GCCCAAAAGAGTCAGAAAAAATGGAGGGATTATTACACCAAATCCATCGGACACGGGAAG 180
||||||||||||||| |||||||||||||||||||||||||||||||||||||| |||||
4427R-1.in silico 121 GCCCAAAAGAGTCAG-AAAAATGGAGGGATTATTACACCAAATCCATCGGACAC-GGAAG 180
4427R-1.IR_full 181 ACGAGGCTCCCCGAAATNAGCGGTTCCCAACAAGAAACCCCGTTTGGAACAACTTGCTAT 240
|||||||| |||||||| ||||||||||||||||||||||||||||||||||| ||||||
4427R-1.in silico 181 ACGAGGCT-CCCGAAAT-AGCGGTTCCCAACAAGAAACCCCGTTTGGAACAACCTGCTAT 240
4427R-1.IR_full 241 GAGCATGACACCGCCGCCCGGATCAAAAGCTGGACGATGATCAGAAAGCGGAAAGGGTTA 300
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
4427R-1.in silico 241 GAGCATGACACCGCCG-CCGGATCAAAAGCTGGACGATGATCAGAAAGCGGAAAGGGTTA 300
4427R-1.IR_full 301 GCGTCATCATGCGGGTCAACAGTTCTGGCGCTGTCTCTTCTAGTAGCCAAGACGAGAACT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-1.in silico 301 GCGTCATCATGCGGGTCAACAGTTCTGGCGCTGTCTCTTCTAGTAGCCAAGACGAGAACT 360
4427R-1.IR_full 361 CATCTAGTTCCACCTCCTGCTGTAGTTCCTCTTCCAACACAAACACAAGTACAAGTTCAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-1.in silico 361 CATCTAGTTCCACCTCCTGCTGTAGTTCCTCTTCCAACACAAACACAAGTACAAGTTCAG 420
4427R-1.IR_full 421 TACCACCCACTGTGGAGGACGACTATCCAGAGGCCAATGTGTGGCGCAATCTCAAGTTCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4427R-1.in silico 421 TACCACCCACTGTGGAGGACGACTATCCAGAGGCCAATGTGTGGCGCAATCTCAAGTTCA 480
4427R-1.IR_full 481 AAATGAACAGAAAGCGTGCTGCAGAA 506
||||||||||||||||||||||||||
4427R-1.in silico 481 AAATGAACAGAAAGCGTGCTGCAGAA 506
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_164384.1 |
CG4427-RA, transcript variant A (cbt), mRNA |
| 87.75 |
423 |
1 |
1 |
1 |
NM_134685.2 |
CG4427-RB, transcript variant B (cbt), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_141003.2 |
CG3634-RA (CG3634), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_140561.3 |
CG5931-RA (CG5931), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_136598.2 |
CG8196-RA (Ance-4), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_138158.2 |
CG1212-RA, transcript variant A (p130CAS), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_136021.2 |
CG15152-RA (CG15152), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_139461.1 |
CG15877-RA (CG15877), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_134479.1 |
CG14205-RA (CG14205), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_144347.2 |
CG12149-RA (c12.2), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166612.2 |
CG30182-RA (CG30182), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165934.1 |
CG8776-RA, transcript variant A (CG8776), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136702.2 |
CG18445-RA (CG18445), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080214.2 |
CG10913-RA (Spn6), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_134494.2 |
CG12235-RA (Arp11), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_167380.1 |
CG32628-RA (CG32628), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001042932.1 |
CG17494-RB, transcript variant B (CG17494), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001042933.1 |
CG17494-RC, transcript variant C (CG17494), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001042934.1 |
CG17494-RA, transcript variant A (CG17494), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139856.2 |
CG8580-RA, transcript variant A (bhr), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_168211.1 |
CG8580-RB, transcript variant B (bhr), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_079413.2 |
CG4345-RA (grim), mRNA |
| 0 |
0 |
0 |
9 |
12 |
NM_079067.2 |
CG11949-RA, transcript variant A (cora), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_169578.1 |
CG3307-RC, transcript variant C (pr-set7), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_169577.1 |
CG3307-RB, transcript variant B (pr-set7), mRNA |
| 0 |
0 |
0 |
8 |
4 |
NM_142097.2 |
CG3307-RA, transcript variant A (pr-set7), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_169845.1 |
CG31043-RA, transcript variant A (gukh), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_057511.3 |
CG3936-RA (N), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_132529.2 |
CG2025-RA (CG2025), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_166337.1 |
CG11949-RB, transcript variant B (cora), mRNA |
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