NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 4184R-4

Symbol MED15 Full Name Mediator complex subunit 15

CG No CG4184 Old CG No CG4184

Synonyms Arc105, Med9/ARC105, dARC105, CG4184, BcDNA:GH03922, MED15

Accession No (Link to NCBI) NM_134684.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees larval lethal

Map Viewer

Reference
[Please submit your publication] Terriente-Félix A, López-Varea A, de Celis JF.
Identification of genes affecting wing patterning through a loss-of-function mutagenesis screen and characterization of med15 function during wing development.
Genetics (2010) 185(2) 671-84 [ PubMed ID = 20233856 ] [ RRC reference ]

Rodriguez I.
Drosophila TIEG is a modulator of different signalling pathways involved in wing patterning and cell proliferation.
PLoS One (2011) 6(4) e18418 [ PubMed ID = 21494610 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgATGACCGAGGACTGGCAGAGT

Primer Seq. 3'

aatctagaggtaccACCTGCATCATCTGCTGGGCA

Predicted Fragment Size

IR fragment full Seq

0001 ATGACCGAGG ACTGGCAGAG TCAAAAGTTT CGTCAGAATG TCATCTCCAA AATCCACGAC 
0061 TTATTGCCAC CGAATGCGCA GGACCAGACT AAAAATGCCG GCGTTATGGA GAACCCACAT 
0121 ATTTAGAAAG TCGCGTACTA AGGACGAGTA TTTAGGTCTG GTAGCCAAGC TCTTTATGCA 
0181 TTACAAAGAC ATGTCACGAA AGTCCCAGCA ACAGCAGCAG CAGCAACAAC AGCAGGGTGG 
0241 CCCGCCCCCA AATGCGGAAA TGGGCGGCGG GCAGAATATG ATGCAGGATC CACTGAACGC 
0301 ACTTCAGAAT CTTGCCAGTC AAGGAAATCG CAATCCCCAG ATGATGCCCA TGGGCGCCGG 
0361 AGGAGGAGCG CCAGTGCCCG GTGGCCCCGG AACTGCCTCT AACTTGCTAC AATCCCCTGA 
0421 ATCAGCAGCG CCCTGGACAG CAGCAAAATG CAGCCCAATG TCAAAATATC CGTGGCCAAA 
0481 TGGCCCATGG GGTGCCGGAG GNGCTNGGGT GCCCAGCAGA TGATGCAGGT

in silico PCR Fragment

0001 ATGACCGAGG ACTGGCAGAG TCAAAAGTTT CGTCAGAATG TCATCTCCAA AATCCACGAC 
0061 TTATTGCCAC CGAATGCGCA GGACCAGACT AAAAATGCCG GCGTTATGGA GAA-CCACAT 
0121 ATTTCGAAAG TCGCGTACTA AGGACGAGTA TTTAGGTCTG GTAGCCAAGC TCTTTATGCA 
0181 TTACAAAGAC ATGTCACGAA AGTCCCAGCA ACAGCAGCAG CAGCAACAAC AGCAGGGTGG 
0241 CCCGCCCCCA AATGCGGAAA TGGGCGGCGG GCAGAATATG ATGCAGGATC CACTGAACGC 
0301 ACTTCAGAAT CTTGCCAGTC AAGGAAATCG CAATCCCCAG ATGATGCCCA TGGGCGCCGG 
0361 AGGAGGAGCG CCAGTGCCCG GTGGCCCCGG AACTGCCTCT AACTTGCTAC AAT-CCCTGA 
0421 ATCAGCAGCG CCCTGGACAG CAGC-AAATG CAGCCC-ATG TC-AAATATC CGTGGCCAAA 
0481 T-GCCCAT-G GGTGCCGGAG GAGCT--GGT GCCCAGCAGA TGATGCAGGT

Assemble Data


4184R-4.IR_full       1   ATGACCGAGGACTGGCAGAGTCAAAAGTTTCGTCAGAATGTCATCTCCAAAATCCACGAC 60
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4184R-4.in silico     1   ATGACCGAGGACTGGCAGAGTCAAAAGTTTCGTCAGAATGTCATCTCCAAAATCCACGAC 60


4184R-4.IR_full       61  TTATTGCCACCGAATGCGCAGGACCAGACTAAAAATGCCGGCGTTATGGAGAACCCACAT 120
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
4184R-4.in silico     61  TTATTGCCACCGAATGCGCAGGACCAGACTAAAAATGCCGGCGTTATGGAGAA-CCACAT 120


4184R-4.IR_full       121 ATTTAGAAAGTCGCGTACTAAGGACGAGTATTTAGGTCTGGTAGCCAAGCTCTTTATGCA 180
                          |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
4184R-4.in silico     121 ATTTCGAAAGTCGCGTACTAAGGACGAGTATTTAGGTCTGGTAGCCAAGCTCTTTATGCA 180


4184R-4.IR_full       181 TTACAAAGACATGTCACGAAAGTCCCAGCAACAGCAGCAGCAGCAACAACAGCAGGGTGG 240
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4184R-4.in silico     181 TTACAAAGACATGTCACGAAAGTCCCAGCAACAGCAGCAGCAGCAACAACAGCAGGGTGG 240


4184R-4.IR_full       241 CCCGCCCCCAAATGCGGAAATGGGCGGCGGGCAGAATATGATGCAGGATCCACTGAACGC 300
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4184R-4.in silico     241 CCCGCCCCCAAATGCGGAAATGGGCGGCGGGCAGAATATGATGCAGGATCCACTGAACGC 300


4184R-4.IR_full       301 ACTTCAGAATCTTGCCAGTCAAGGAAATCGCAATCCCCAGATGATGCCCATGGGCGCCGG 360
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
4184R-4.in silico     301 ACTTCAGAATCTTGCCAGTCAAGGAAATCGCAATCCCCAGATGATGCCCATGGGCGCCGG 360


4184R-4.IR_full       361 AGGAGGAGCGCCAGTGCCCGGTGGCCCCGGAACTGCCTCTAACTTGCTACAATCCCCTGA 420
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||
4184R-4.in silico     361 AGGAGGAGCGCCAGTGCCCGGTGGCCCCGGAACTGCCTCTAACTTGCTACAAT-CCCTGA 420


4184R-4.IR_full       421 ATCAGCAGCGCCCTGGACAGCAGCAAAATGCAGCCCAATGTCAAAATATCCGTGGCCAAA 480
                          |||||||||||||||||||||||| ||||||||||| ||||| |||||||||||||||||
4184R-4.in silico     421 ATCAGCAGCGCCCTGGACAGCAGC-AAATGCAGCCC-ATGTC-AAATATCCGTGGCCAAA 480


4184R-4.IR_full       481 TGGCCCATGGGGTGCCGGAGGNGCTNGGGTGCCCAGCAGATGATGCAGGT 530
                          | |||||| |||||||||||| |||  |||||||||||||||||||||||
4184R-4.in silico     481 T-GCCCAT-GGGTGCCGGAGGAGCT--GGTGCCCAGCAGATGATGCAGGT 530

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	503	8	15	40	NM_134684.2	CG4184-RA (MED15), mRNA
11.92	60	360	915	1378	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
11.92	60	360	915	1378	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
11.92	60	360	915	1378	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
6.36	32	49	126	204	NM_079939.2	CG5580-RA, transcript variant A (sbb), mRNA
5.76	29	123	366	690	NM_166030.1	CG8118-RB, transcript variant B (mam), mRNA
5.76	29	123	366	690	NM_080376.1	CG8118-RA, transcript variant A (mam), mRNA
5.76	29	81	152	293	NM_169258.1	CG9755-RA, transcript variant A (pum), mRNA
5.76	29	81	152	293	NM_169259.1	CG9755-RD, transcript variant D (pum), mRNA
5.76	29	81	152	293	NM_079561.2	CG9755-RC, transcript variant C (pum), mRNA
5.56	28	71	171	368	NM_206286.1	CG10107-RC, transcript variant C (CG10107), mRNA
5.56	28	71	171	368	NM_139799.3	CG10107-RA, transcript variant A (CG10107), mRNA
4.57	23	69	186	308	NM_132246.2	CG10555-RA (CG10555), mRNA
4.57	23	56	134	202	NM_166992.2	CG2904-RA (ec), mRNA
4.37	22	107	264	518	NM_135077.2	CG14023-RA (CG14023), mRNA
4.17	21	58	130	199	NM_132126.1	CG3075-RA (CG3075), mRNA
4.17	21	45	119	278	NM_001014575.1	CG7391-RE, transcript variant E (Clk), mRNA
4.17	21	45	119	278	NM_001014574.1	CG7391-RF, transcript variant F (Clk), mRNA
4.17	21	45	119	278	NM_001014576.1	CG7391-RD, transcript variant D (Clk), mRNA
4.17	21	45	119	278	NM_079240.2	CG7391-RA, transcript variant A (Clk), mRNA
4.17	21	45	119	278	NM_206299.1	CG7391-RC, transcript variant C (Clk), mRNA
4.17	21	45	119	278	NM_170628.1	CG7391-RB, transcript variant B (Clk), mRNA
3.97	20	129	290	587	NM_168571.2	CG32133-RA (CG32133), mRNA
3.77	19	59	138	324	NM_176556.1	CG33106-RA, transcript variant A (mask), mRNA
3.77	19	59	138	324	NM_176557.1	CG33106-RB, transcript variant B (mask), mRNA
3.57	18	77	225	461	NM_131975.3	CG32767-RA, transcript variant A (CG32767), mRNA
3.57	18	77	225	461	NM_206628.1	CG32767-RB, transcript variant B (CG32767), mRNA
3.57	18	59	113	234	NM_132004.2	CG4136-RA (CG4136), mRNA
3.57	18	43	131	226	NM_206357.2	CG33261-RD, transcript variant D (Trl), mRNA
3.57	18	40	104	152	NM_206360.1	CG33261-RF, transcript variant F (Trl), mRNA

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