Figure 1

Mitotic metaphase chromosomes of the 1H monosomic addition line (42 wheat chromosome plus one barley chromosome).  Barley chromosome 1H (indicated by a white arrow) is detected by GISH with barley genomic DNA probe (red). The green signals are FISH signals of the barley specific subtelomeric sequence HvT01 (Belostotsky and Ananiev 1990). Chromsome 1H of barley has a bright FISH signal on the short arm and slightly reduced signal on the long arm. Chromosomes are counter-stained by 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, 1.5 μg/ml). White bar indicates 10 μm.