|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
non-essential
|
|
References (PMID)
|
FEMS Microbiol Lett. 1996;135(2-3):275-80.
4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.
Zhao G, Winkler ME.
(
8595869
)
|
|
|
Deletion
|
OCR27
(D)
,
OCR27-1
(D)
,
OCR27-6
(D)
,
OCR27-7
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
thrB
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
thrB
Search strains by all related name:
thrB b0003 ECK0003 JW0002
|
General information (Go to Linear View: )
|
|
Gene Name
|
thrB
|
|
Alternative name
|
b0003,ECK0003,JW0002
|
|
Location, Length
|
2,801 - 3,733
(
+
)
;
0.06
min
;
933
(bp) ,
310
(aa)
|
|
Product
|
homoserine kinase
|
|
Operon Name
|
thrLABC
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009088 - threonine biosynthetic process
|
|
Function
|
enzyme; Amino acid biosynthesis: Threonine,1.5.1.8 metabolism; building block biosynthesis; amino acids; threonine,7.1 location of gene products; cytoplasm
|
|
Gene Ontology
|
GO:0000166
;
nucleotide binding ( thrB )
GO:0004413
;
homoserine kinase activity ( thrB )
GO:0005524
;
ATP binding ( thrB )
GO:0005737
;
cytoplasm ( thrB )
GO:0006566
;
threonine metabolic process ( thrB )
GO:0008652
;
amino acid biosynthetic process ( thrB )
GO:0009088
;
threonine biosynthetic process ( thrB )
GO:0016301
;
kinase activity ( thrB )
GO:0016310
;
phosphorylation ( thrB )
GO:0016740
;
transferase activity ( thrB )
|
|
PID
|
1786184
|
EC number
(KEGG Pathway)
|
2.7.1.39
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
KHSE_ECOLI
(
P00547
)
|
|
- Protein name
|
Homoserine kinase
|
|
- Synonyms
|
EC 2.7.1.39, HK
|
|
- Gene name
|
Name=thrB; OrderedLocusNames=b0003;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
thrB
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, Crystal Structure Of Galactokinase From Pyrococcus Horikoshi
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Homoserine kinase
|
|
PSI-BLAST
|
Homoserine kinase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
homoserine kinase [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
GLYCOSAMINOGLYCAN
CK2_PHOSPHO_SITE
MYRISTYL
PROKAR_LIPOPROTEIN
GHMP_KINASES_ATP
|
|
MMBR References
|
J Bacteriol. 1979;140(1):106-13.
Structural and functional analysis of cloned deoxyribonucleic acid containing the trpR-thr region of the Escherichia coli chromosome.
Gunsalus RP, Zurawski G, Yanofsky C.
(
159284
)
|
J Bacteriol. 1985;161(1):461-2.
Evidence for an internal promoter in the Escherichia coli threonine operon.
Saint Girons I, Margarita D.
(
3918011
)
|
FEMS Microbiol Lett. 1996;135(2-3):275-80.
4-Phospho-hydroxy-L-threonine is an obligatory intermediate in pyridoxal 5'-phosphate coenzyme biosynthesis in Escherichia coli K-12.
Zhao G, Winkler ME.
(
8595869
)
|
Nucleic Acids Res. 1981;9:339-347
Nucleotide sequence of the thrB gene of E. coli, and its two adjacent regions; the thrAB and thrBC junctions.
Cossart, P., M. Katinka, and M. Yaniv.
|
Mol Gen Genet. 1979;175(1):39-44.
Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes.
Cossart P, Katinka M, Yaniv M, Saint Girons I, Cohen GN.
(
390305
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG RFADKLPSEP RENIVYQCWE
0071 RFCQELGKQI PVAMTLEKNM PIGSGLGSSA CSVVAALMAM NEHCGKPLND TRLLALMGEL EGRISGSIHY
0141 DNVAPCFLGG MQLMIEENDI ISQQVPGFDE WLWVLAYPGI KVSTAEARAI LPAQYRRQDC IAHGRHLAGF
0211 IHACYSRQPE LAAKLMKDVI AEPYRERLLP GFRQARQAVA EIGAVASGIS GSGPTLFALC DKPETAQRVA
0281 DWLGKNYLQN QEGFVHICRL DTAGARVLEN
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 CCGTTGGTAC TGCGCGGATA TGGTGCGGGC
-070 AATGACGTTA CAGCTGCCGG TGTCTTTGCT GATCTGCTAC GTACCCTCTC ATGGAAGTTA GGAGTCTGAC
0001 atggttaaag tttatgcccc ggcttccagt gccaatatga gcgtcgggtt tgatgtgctc ggggcggcgg
0071 tgacacctgt tgatggtgca ttgctcggag atgtagtcac ggttgaggcg gcagagacat tcagtctcaa
0141 caacctcgga cgctttgccg ataagctgcc gtcagaacca cgggaaaata tcgtttatca gtgctgggag
0211 cgtttttgcc aggaactggg taagcaaatt ccagtggcga tgaccctgga aaagaatatg ccgatcggtt
0281 cgggcttagg ctccagtgcc tgttcggtgg tcgcggcgct gatggcgatg aatgaacact gcggcaagcc
0351 gcttaatgac actcgtttgc tggctttgat gggcgagctg gaaggccgta tctccggcag cattcattac
0421 gacaacgtgg caccgtgttt tctcggtggt atgcagttga tgatcgaaga aaacgacatc atcagccagc
0491 aagtgccagg gtttgatgag tggctgtggg tgctggcgta tccggggatt aaagtctcga cggcagaagc
0561 cagggctatt ttaccggcgc agtatcgccg ccaggattgc attgcgcacg ggcgacatct ggcaggcttc
0631 attcacgcct gctattcccg tcagcctgag cttgccgcga agctgatgaa agatgttatc gctgaaccct
0701 accgtgaacg gttactgcca ggcttccggc aggcgcggca ggcggtcgcg gaaatcggcg cggtagcgag
0771 cggtatctcc ggctccggcc cgaccttgtt cgctctgtgt gacaagccgg aaaccgccca gcgcgttgcc
0841 gactggttgg gtaagaacta cctgcaaaat caggaaggtt ttgttcatat ttgccggctg gatacggcgg
0911 gcgcacgagt actggaaaac taaATGAAAC TCTACAATCT GAAAGATCAC AACGAGCAGG TCAGCTTTGC
0981 GCAAGCCGTA ACCCAGGGGT TGGGCAAAAA TCAGGGGCTG TTTTTTCCGC ACG
|
