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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Bacteriol. 1991;173(15):4625-36.
The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.
Mengin-Lecreulx D, Texier L, Rousseau M, van Heijenoort J.
(
1649817
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
secA
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Related strains
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Strains Search
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Search strains by gene name:
secA
Search strains by all related name:
secA azi b0098 ECK0099 JW0096 pea prlD
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General information (Go to Linear View: )
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Gene Name
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secA
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Alternative name
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azi,b0098,ECK0099,JW0096,pea,prlD
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Location, Length
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108,279 - 110,984
(
+
)
;
2.33
min
;
2706
(bp) ,
901
(aa)
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Product
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preprotein translocase subunit, ATPase
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Operon Name
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secMA-mutT
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Note
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preprotein translocase; secretion protein; GO_component: GO:0005737 - cytoplasm
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Function
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transport; Protein, peptide secretion
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Gene Ontology
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GO:0000166
;
nucleotide binding ( secA )
GO:0005515
;
protein binding ( secA )
GO:0005524
;
ATP binding ( secA )
GO:0005737
;
cytoplasm ( secA )
GO:0005886
;
plasma membrane ( secA )
GO:0006605
;
protein targeting ( secA )
GO:0006810
;
transport ( secA )
GO:0006886
;
intracellular protein transport ( secA )
GO:0008270
;
zinc ion binding ( secA )
GO:0015031
;
protein transport ( secA )
GO:0016020
;
membrane ( secA )
GO:0017038
;
protein import ( secA )
GO:0046872
;
metal ion binding ( secA )
GO:0065002
;
intracellular protein transport across a membrane ( secA )
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PID
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1786287
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EC number
(KEGG Pathway)
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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secA
(
EG10936
)
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Number of removed
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0 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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SECA_ECOLI
(
P10408
;
P75642
)
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- Protein name
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Preprotein translocase secA subunit.
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- Synonyms
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- Gene name
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Name=secA; Synonyms=prlD, azi, pea; OrderedLocusNames=b0098;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
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secA
(
homologous genes of other bacterias
)
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PDB
(database updated :
2007.02.20
)
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PSI-BLAST
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Chain A, Crystal Structure Of Seca:adp In An Open Conformation From Bacillus Subtilis
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SWISS-PROT
(database updated :
2007.02.20
)
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BLAST
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Preprotein translocase subunit secA
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PSI-BLAST
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Preprotein translocase subunit secA
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nr
(database updated :
2007.02.20
)
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BLAST
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translocase [Escherichia coli K12]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
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ASN_GLYCOSYLATION
CAMP_PHOSPHO_SITE
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
SECA
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MMBR References
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J Biol Chem. 1995;270:20106-20111
SecYEG and SecA are the stoichiometric components of preprotein translocase.
Douville, K., A. Price, J. Eichler, A. Economou, and W. Wickner.
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J Bacteriol. 1989;171(1):402-9.
Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide.
Fikes JD, Bassford PJ Jr.
(
2536662
)
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J Bacteriol. 1990;172(11):6607-10.
Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene.
Fortin Y, Phoenix P, Drapeau GR.
(
2146254
)
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J Bacteriol. 1993;175:6113-6117
Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo.
Kosic, N., M. Sugai, C. K. Fan, and H. C. Wu.
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Cell. 1981;25(3):765-72.
E. coli mutant pleiotropically defective in the export of secreted proteins.
Oliver DB, Beckwith J.
(
7026050
)
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J Bacteriol. 1982;150(2):686-91.
Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli.
Oliver DB, Beckwith J.
(
6279567
)
|
Biochem Biophys Res Commun. 1997;235(3):593-7.
RNA helicase activity of Escherichia coli SecA protein.
Park SK, Kim DW, Choe J, Kim H.
(
9207202
)
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J Bioenerg Biomembr. 1990;22(3):291-310.
The sec and prl genes of Escherichia coli.
Bieker KL, Phillips GJ, Silhavy TJ.
(
2202721
)
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RNA 1995;1:745-753
Competition between ribosome and SecA binding promotes Escherichia coli secA translational regulation.
Salavati, R., and D. Oliver.
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J Bacteriol. 1988;170(8):3404-14.
Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli.
Schmidt MG, Rollo EE, Grodberg J, Oliver DB.
(
2841285
)
|
Cell. 1988;55(4):683-92.
SecA protein is required for secretory protein translocation into E. coli membrane vesicles.
Cabelli RJ, Chen L, Tai PC, Oliver DB.
(
2846186
)
|
J Bacteriol. 1994;176:4197-4203
In vivo studies of the role of SecA during protein export in Escherichia coli.
Chun, S.-Y., and L. L. Randall.
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J Bacteriol. 1995;177:4969-4973
Multicopy suppression of cold-sensitive sec mutations in Escherichia coli.
Danese, P. N., C. K. Murphy, and T. J. Silhavy.
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| Sequences |
Amino acid
FASTA format
|
0001 MLIKLLTKVF GSRNDRTLRR MRKVVNIINA MEPEMEKLSD EELKGKTAEF RARLEKGEVL ENLIPEAFAV
0071 VREASKRVFG MRHFDVQLLG GMVLNERCIA EMRTGEGKTL TATLPAYLNA LTGKGVHVVT VNDYLAQRDA
0141 ENNRPLFEFL GLTVGINLPG MPAPAKREAY AADITYGTNN EYGFDYLRDN MAFSPEERVQ RKLHYALVDE
0211 VDSILIDEAR TPLIISGPAE DSSEMYKRVN KIIPHLIRQE KEDSETFQGE GHFSVDEKSR QVNLTERGLV
0281 LIEELLVKEG IMDEGESLYS PANIMLMHHV TAALRAHALF TRDVDYIVKD GEVIIVDEHT GRTMQGRRWS
0351 DGLHQAVEAK EGVQIQNENQ TLASITFQNY FRLYEKLAGM TGTADTEAFE FSSIYKLDTV VVPTNRPMIR
0421 KDLPDLVYMT EAEKIQAIIE DIKERTAKGQ PVLVGTISIE KSELVSNELT KAGIKHNVLN AKFHANEAAI
0491 VAQAGYPAAV TIATNMAGRG TDIVLGGSWQ AEVAALENPT AEQIEKIKAD WQVRHDAVLE AGGLHIIGTE
0561 RHESRRIDNQ LRGRSGRQGD AGSSRFYLSM EDALMRIFAS DRVSGMMRKL GMKPGEAIEH PWVTKAIANA
0631 QRKVESRNFD IRKQLLEYDD VANDQRRAIY SQRNELLDVS DVSETINSIR EDVFKATIDA YIPPQSLEEM
0701 WDIPGLQERL KNDFDLDLPI AEWLDKEPEL HEETLRERIL AQSIEVYQRK EEVVGAEMMR HFEKGVMLQT
0771 LDSLWKEHLA AMDYLRQGIH LRGYAQKDPK QEYKRESFSM FAAMLESLKY EVISTLSKVQ VRMPEEVEEL
0841 EQQRRMEAER LAQMQQLSHQ DDDSAAAAAL AAQTGERKVG RNDPCPCGSG KKYKQCHGRL Q
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GCGCAAGGCA TCCGTGCTGG CCCTCAACGC
-070 CTCACCTAAC AACAATAAAC CTTTACTTCA TTTTATTAAC TCCGCAACGC GGGGCGTTTG AGATTTTATT
0001 atgctaatca aattgttaac taaagttttc ggtagtcgta acgatcgcac cctgcgccgg atgcgcaaag
0071 tggtcaacat catcaatgcc atggaaccgg agatggaaaa actctccgac gaagaactga aagggaaaac
0141 cgcagagttt cgtgcacgtc tggaaaaagg cgaagtgctg gaaaatctga tcccggaagc tttcgccgtg
0211 gtacgtgagg caagtaagcg cgtctttggt atgcgtcact tcgacgttca gttactcggc ggtatggttc
0281 ttaacgaacg ctgcatcgcc gaaatgcgta ccggtgaagg aaaaaccctg accgcaacgc tgcctgctta
0351 cctgaacgca ctaaccggta aaggcgtgca cgtagttacc gtcaacgact acctggcgca acgtgacgcc
0421 gaaaacaacc gtccgctgtt tgaattcctt ggcctgactg tcggtatcaa cctgccgggc atgccagcac
0491 cggcaaagcg cgaagcttac gcagctgaca tcacttacgg tacgaacaac gaatacggct ttgactacct
0561 gcgcgacaac atggcgttca gccctgaaga acgtgtacag cgtaaactgc actatgcgct ggtggacgaa
0631 gtggactcca tcctgatcga tgaagcgcgt acaccgctga tcatttccgg cccggcagaa gacagctcgg
0701 aaatgtataa acgcgtgaat aaaattattc cgcacctgat ccgtcaggaa aaagaagact ccgaaacctt
0771 ccagggcgaa ggccacttct cggtggacga aaaatctcgc caggtgaacc tgaccgaacg tggtctggtg
0841 ctgattgaag aactgctggt gaaagagggc atcatggatg aaggggagtc tctgtactct ccggccaaca
0911 tcatgctgat gcaccacgta acggcggcgc tgcgcgctca tgcgctgttt acccgtgacg tcgactacat
0981 cgttaaagat ggtgaagtta tcatcgttga cgaacacacc ggtcgtacca tgcagggccg tcgctggtcc
1051 gatggtctgc accaggctgt ggaagcgaaa gaaggtgtgc agatccagaa cgaaaaccaa acgctggctt
1121 cgatcacctt ccagaactac ttccgtctgt atgaaaaact ggcggggatg accggtactg ctgataccga
1191 agctttcgaa tttagctcaa tctacaagct ggataccgtc gttgttccga ccaaccgtcc aatgattcgt
1261 aaagatctgc cggacctggt ctacatgact gaagcggaaa aaattcaggc gatcattgaa gatatcaaag
1331 aacgtactgc gaaaggccag ccggtgctgg tgggtactat ctccatcgaa aaatcggagc tggtgtcaaa
1401 cgaactgacc aaagccggta ttaagcacaa cgtcctgaac gccaaattcc acgccaacga agcggcgatt
1471 gttgctcagg caggttatcc ggctgcggtg actatcgcga ccaatatggc gggtcgtggt acagatattg
1541 tgctcggtgg tagctggcag gcagaagttg ccgcgctgga aaatccgacc gcagagcaaa ttgaaaaaat
1611 taaagccgac tggcaggtac gtcacgatgc ggtactggaa gcaggtggcc tgcatatcat cggtaccgag
1681 cgtcacgaat cccgtcgtat cgataaccag ttgcgcggtc gttctggtcg tcagggggat gctggttctt
1751 cccgtttcta cctgtcgatg gaagatgcgc tgatgcgtat ttttgcttcc gaccgagtat ccggcatgat
1821 gcgtaaactg ggtatgaagc caggcgaagc cattgaacac ccgtgggtga ctaaagcgat tgccaacgcc
1891 cagcgtaaag ttgaaagccg taacttcgac attcgtaagc aactgctgga atatgatgac gtggctaacg
1961 atcagcgtcg cgccatttac tcccagcgta acgaactgtt ggatgtcagc gatgtgagcg aaaccattaa
2031 cagcattcgt gaagatgtgt tcaaagcgac cattgatgcc tacattccac cacagtcgct ggaagaaatg
2101 tgggatattc cggggctgca ggaacgtctg aagaacgatt tcgacctcga tttgccaatt gccgagtggc
2171 tggataaaga accagaactg catgaagaga cgctgcgtga gcgcattctg gcgcagtcca tcgaagtgta
2241 tcagcgtaaa gaagaagtgg ttggtgctga gatgatgcgt cacttcgaga aaggcgtcat gctgcaaacg
2311 cttgactccc tgtggaaaga gcacctggca gcgatggact atctgcgtca gggtatccac ctgcgtggct
2381 acgcacagaa agatccgaag caggaataca aacgtgaatc gttctccatg tttgcagcga tgctggagtc
2451 gttgaaatat gaagttatca gtacgctgag caaagttcag gtacgtatgc ctgaagaggt tgaggagctg
2521 gaacaacagc gtcgtatgga agccgagcgt ttagcgcaaa tgcagcagct tagccatcag gatgacgact
2591 ctgcagccgc agctgcactg gcggcgcaaa ccggagagcg caaagtagga cgtaacgatc cttgcccgtg
2661 cggttctggt aaaaaataca agcagtgcca tggccgcctg caataaAAGC TAACTGTTGA AGTAAAAGGC
2731 GCAGGATTCT GCGCCTTTTT TATAGGTTTA AGACAATGAA AAAGCTGCAA ATTGCGGTAG GTATTATTCG
2801 CAACGA
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