MG1655
W3110
Search for
Japanese | English
Gene Report : rpsB
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
J Bacteriol. 1997;179(20):6228-37.
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.
Link AJ, Phillips D, Church GM. ( 9335267 )
     Deletion OCR33,34-1 (D)  ,  OCR33,34-2 (D)  ,  OCR33,35-4 (AK4-2 / BK5-2) (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  rpsB
Related strains
     Strains Search Search strains by gene name:  rpsB   Search strains by all related name:  rpsB b0169 ECK0168 JW0164

General information  (Go to Linear View:)
 Gene Name rpsB  
 Alternative name b0169,ECK0168,JW0164  
 Location, Length 189,874 - 190,599 (  +  ) ;   4.09 min ; 726 (bp) ,   241 (aa) Go to Linear View
 Product 30S ribosomal subunit protein S2  
 Operon Name tff-rpsB-tsf  
 Note GO_component: GO:0005737 - cytoplasm; GO_function: GO:0003735 - structural constituent of ribosome; GO_process: GO:0006412 - translation  
 Function structural component; Ribosomal proteins - synthesis, modification  
 Gene Ontology GO:0003735 ; structural constituent of ribosome ( rpsB )
GO:0005515 ; protein binding ( rpsB )
GO:0005622 ; intracellular ( rpsB )
GO:0005840 ; ribosome ( rpsB )
GO:0006412 ; translation ( rpsB )
GO:0015935 ; small ribosomal subunit ( rpsB )
GO:0030529 ; ribonucleoprotein complex ( rpsB )
 PID 1786365  
 EC number
  (KEGG Pathway)
 
 
Verified Protein Starts (data compiled from literature and appropriate citations are available from EcoGene)
     EcoGene rpsB ( EG10901 )  
    Number of removed 1 aa cleaved  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RS2_ECOLI ( P02351 ; Q9R2E5 )
    -  Protein name 30S ribosomal protein S2.  
    -  Synonyms  
    -  Gene name Name=rpsB; OrderedLocusNames=b0169, c0204, z0180, ECs0171;  

 Linear View (Whole Mode)
View Location
   180.0  –  205.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP rpsB (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain B, Crystal Structure Of The Bacterial Ribosome From Escherichia Coli In Complex With The Antibiotic Kasugamyin At 3.5a Resolution. This File Contains The 30s Subunit Of One 70s Ribosome. The Entire Crystal Structure Contains Two 70s Ribosomes And Is  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST 30S ribosomal protein S2  
         PSI-BLAST 30S ribosomal protein S2
    nr     (database updated : 2007.02.20 )
         BLAST 30S ribosomal protein S2 [Escherichia coli CFT073]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    MYRISTYL    RIBOSOMAL_S2_1    RIBOSOMAL_S2_2     

Other Cross-Reference
    EcoCyc rpsB 

MMBR References
Mol Gen Genet. 1981;182(1):178-9.
Revised location of the Escherichia coli gene coding for ribosomal protein S2.
Lathe R, Bollen A, Herzog R. ( 7022135 )
Mol Gen Genet. 1981;181(3):356-62.
Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed.
Bendiak DS, Friesen JD. ( 7017348 )
J Mol Biol. 1975;96(3):443-53.
Late steps in the assembly of 30 S ribosomal proteins in vivo in a spectinomycin-resistant mutant of Escherichia coli.
Nashimoto H, Uchida H. ( 126327 )
J Mol Biol. 1979;132(2):219-33.
A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).
Bollen A, Lathe R, Herzog A, Denicourt D, Lecocq JP, Desmarez L, Lavalle R. ( 161328 )
Nucleic Acids Res. 1981;9:4163-4172
Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts.
An, G., D. S. Bendiak, L. A. Mamelak, and J. D. Friesen.

Sequences
Amino acid
FASTA format
0001 MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV 
0071 GTKRAASEAV KDAALSCDQF FVNHRWLGGM LTNWKTVRQS IKRLKDLETQ SQDGTFDKLT KKEALMRTRE 
0141 LEKLENSLGG IKDMGGLPDA LFVIDADHEH IAIKEANNLG IPVFAIVDTN SDPDGVDFVI PGNDDAIRAV 
0211 TLYLGAVAAT VREGRSQDLA SQAEESFVEA E

Nucleotide
FASTA format

View sequence out neighbor 100bp
-100                                             CAACACACAC GTATCGGCAC ATATTCCGGG 
-070 GTGCCCTTTG GGGTCGGTAA TATGGGATAC GTGGAGGCAT AACCCCAACT TTTATATAGA GGTTTTAATC 
0001 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga 
0071 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt 
0141 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt 
0211 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc 
0281 atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct 
0351 ggaaactcag tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag 
0421 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa 
0491 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt 
0561 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg 
0631 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct tcccaggcgg 
0701 aagaaagctt cgtagaagct gagtaaTAAG GCTTGATAAC TCCCCCAAAA TAGTTCGAGT TGCAGAAAGG 
0771 CGGCAAGCTC GAGAATTCCC GGGAGCTTAC ATCAGTAAGT GACCGGGATG AGCGAG