MG1655
W3110
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Gene Report : ribF
PEC Original Annotations
Essentiality
     Class essential
     References (PMID)
Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
     Deletion OCR27 (D)  ,  OCR27-9 (D)  
Related gene (W3110 PEC)
     Gene Search Search MG1655 PEC by gene name:  ribF
Related strains
     Strains Search Search strains by gene name:  ribF   Search strains by all related name:  ribF b0025 ECK0026 JW0023 o312 yaaC

General information  (Go to Linear View:)
 Gene Name ribF  
 Alternative name b0025,ECK0026,JW0023,o312,yaaC  
 Location, Length 21,407 - 22,348 (  +  ) ;   0.46 min ; 942 (bp) ,   313 (aa) Go to Linear View
 Product bifunctional riboflavin kinase/FAD synthetase  
 Operon Name ribF-ileS-lspA-fkpB-ispH  
 Note GO_component: GO:0005737 - cytoplasm  
 Function putative regulator; Not classified  
 Gene Ontology GO:0003824 ; catalytic activity ( ribF )
GO:0003919 ; FMN adenylyltransferase activity ( ribF )
GO:0005515 ; protein binding ( ribF )
GO:0008531 ; riboflavin kinase activity ( ribF )
GO:0009231 ; riboflavin biosynthetic process ( ribF )
GO:0016740 ; transferase activity ( ribF )
GO:0016779 ; nucleotidyltransferase activity ( ribF )
 PID 1786208  
 EC number
  (KEGG Pathway)   		2.7.1.26   2.7.7.2  
SWISS-PROT  ( Show details )
  botton Entry name(Acc.no) RIBF_ECOLI ( P08391 ; P75621 )
    -  Protein name Riboflavin biosynthesis protein ribF  
    -  Synonyms  
    -  Gene name OrderedLocusNames=b0025, c0029, z0029, ECs0028, SF0021, S0024;  

 Linear View (Whole Mode)
View Location
   10.0  –  35.0 (KBP)

Homology Analysis
BLAST
    Bacteria
         GTOP ribF (  homologous genes of other bacterias  )  
    PDB     (database updated : 2007.02.20 )
         PSI-BLAST Chain A, Crystal Structure Of A Flavin Binding Protein From Thermotoga Maritima, Tm379  
    SWISS-PROT     (database updated : 2007.02.20 )
         BLAST Riboflavin biosynthesis protein ribF [Includes: Riboflavin kinase (Flavokinase); FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)]  
         PSI-BLAST Riboflavin biosynthesis protein ribF [Includes: Riboflavin kinase (Flavokinase); FMN adenylyltransferase (FAD pyrophosphorylase) (FAD synthetase)]
    nr     (database updated : 2007.02.20 )
         BLAST hypothetical protein Z0029 [Escherichia coli O157:H7 EDL933]  
Pfam 28.0   (database updated : 2015-05 )
    Pfam
PROSITE
    PROSITE ASN_GLYCOSYLATION    PKC_PHOSPHO_SITE    CK2_PHOSPHO_SITE    TYR_PHOSPHO_SITE    MYRISTYL    AMIDATION     

Other Cross-Reference
    COG COG0196H 
    EcoCyc ribF 

MMBR References
In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 1. ASM Press, Was 1996:657-664
Biosynthesis of riboflavin.
Bacher, A., S. Eberhardt, and G. Richter.

Sequences
Amino acid
FASTA format 
0001 MKLIRGIHNL SQAPQEGCVL TIGNFDGVHR GHRALLQGLQ EEGRKRNLPV MVMLFEPQPL ELFATDKAPA 
0071 RLTRLREKLR YLAECGVDYV LCVRFDRRFA ALTAQNFISD LLVKHLRVKF LAVGDDFRFG AGREGDFLLL 
0141 QKAGMEYGFD ITSTQTFCEG GVRISSTAVR QALADDNLAL AESLLGHPFA ISGRVVHGDE LGRTIGFPTA 
0211 NVPLRRQVSP VKGVYAVEVL GLGEKPLPGV ANIGTRPTVA GIRQQLEVHL LDVAMDLYGR HIQVVLRKKI 
0281 RNEQRFASLD ELKAQIARDE LTAREFFGLT KPA
Nucleotide
FASTA format

View sequence out neighbor 100bp 
-100                                             ATGAAAAGCC ACTTCTTTCT GATTTCGGTA 
-070 CTCAATCGCC GGTTAACCTT GACCGCTGTA CAAGGTATAC TCGGACGATT TTCACTGTTT TGAGCCAGAC 
0001 atgaagctga tacgcggcat acataatctc agccaggccc cgcaagaagg gtgtgtgctg actattggta 
0071 atttcgacgg cgtgcatcgc ggtcatcgcg cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa 
0141 cttaccggtg atggtgatgc tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca 
0211 agactgaccc ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg ctgtgcgtgc 
0281 gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat cttctggtga agcatttgcg 
0351 cgtaaaattt cttgccgtag gtgatgattt ccgctttggc gctggtcgtg aaggcgattt cttgttatta 
0421 cagaaagctg gcatggaata cggcttcgat atcaccagta cgcaaacttt ttgcgaaggt ggcgtgcgca 
0491 tcagcagcac cgccgtgcgt caggcccttg cggatgacaa tctggctctg gcagagagtt tactggggca 
0561 cccgtttgcc atctccgggc gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg 
0631 aatgtaccgc tgcgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg ggcctcggtg 
0701 aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc ggtattcgcc agcagctgga 
0771 agtgcatttg ttagatgttg caatggacct ttacggtcgc catatacaag tagtgctgcg taaaaaaata 
0841 cgcaatgagc agcgatttgc gtcgctggac gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc 
0911 gcgaattttt tgggctaaca aaaccggctt aaGCCTGTTA TGTAATCAAA CCGAAATACG GAACCGAGAA 
0981 TCTGATGAGT GACTATAAAT CAACCCTGAA TTTGCCGGAA ACAGGGTTCC CGATGCGTGG CG