PEC Original Annotations
|
Essentiality
|
Class
|
non-essential
|
References (PMID)
|
J Exp Biol. 1994;196:443-56.
Molecular physiology of the Na+/H+ antiporter in Escherichia coli.
Padan E, Schuldiner S.
(
7823039
)
|
|
Deletion
|
OCR27
(D)
,
OCR27-8
(D)
|
Related gene (W3110 PEC)
|
Gene Search
|
Search MG1655 PEC by gene name:
nhaR
|
Related strains
|
Strains Search
|
Search strains by gene name:
nhaR
Search strains by all related name:
nhaR antO b0020 ECK0021 JW0019 yaaB
|
General information (Go to Linear View:)
|
Gene Name
|
nhaR
|
Alternative name
|
antO,b0020,ECK0021,JW0019,yaaB
|
Location, Length
|
18,715 - 19,620
(
+
)
;
0.40
min
;
906
(bp) ,
301
(aa)
|
Product
|
DNA-binding transcriptional activator
|
Operon Name
|
nhaAR
|
Note
|
transcriptional activator of nhaA; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0016563 - transcription activator activity; GO_process: GO:0006350 - transcription; GO_process: GO:0009268 - response to pH
|
Function
|
regulator; Transport of small molecules: Cations
|
Gene Ontology
|
GO:0003677
;
DNA binding ( nhaR )
GO:0003700
;
transcription factor activity ( nhaR )
GO:0005515
;
protein binding ( nhaR )
GO:0005737
;
cytoplasm ( nhaR )
GO:0006350
;
transcription ( nhaR )
GO:0006355
;
regulation of transcription, DNA-dependent ( nhaR )
|
PID
|
1786202
|
EC number
(KEGG Pathway)
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
NHAR_ECOLI
(
P10087
;
P75619; Q47409; Q83SR2
)
|
- Protein name
|
Transcriptional activator protein nhaR
|
- Synonyms
|
Na+/H+ antiporter regulatory protein
|
- Gene name
|
Name=nhaR; Synonyms=antO; OrderedLocusNames=b0020, SF0017, S0019;
|
MMBR References
|
J Bioenerg Biomembr. 1993;25(6):647-69.
Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells.
Padan E, Schuldiner S.
(
8144493
)
|
J Biol Chem. 1992;267:10433-10438
NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR) regulates nhaA, the sodium proton antiporter gene in Escherichia coli.
Rahav-Manor, O., O. Carmel, R. Karpel, D. Taglicht, G. Glaser, S. Schuldiner, and E. Padan.
|
EMBO J. 1994;13:1981-1989
A single amino acid substitution (Glu134Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli.
Carmel, O., N. Dover, O. Rahav-Manor, P. Dibrov, D. Kirsch, R. Karpel, S. Schuldiner, and E. Padan.
|
Sequences |
Amino acid
FASTA format
|
0001 MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG KLFKRKGRGL EPSELGELVY
0071 RYADKMFTLS QEMLDIVNYR KESNLLFDVG VADALSKRLV SSVLNAAVVE GEPIHLRCFE STHEMLLEQL
0141 SQHKLDMIIS DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP PEKPFPACLE ERRLLIPGRR SMLGRKLLNW
0211 FNSQGLNVEI LGEFDDAALM KAFGAMHNAI FVAPTLYAYD FYADKTVVEI GRVENVMEEY HAIFAERMIQ
0281 HPAVQRICNT DYSALFSPAV R
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GATACAGCTG GTTACGCGTT CGTTTGCGTC
-070 CATCAGTTTG ACAGGACGGT TTACCGGGGA GCCATAAACG GCTCCCTTTT CATTGTTATC AGGGAGAGAA
0001 atgagcatgt ctcatatcaa ttacaaccac ttgtattact tctggcatgt ctataaagaa ggttccgtgg
0071 ttggcgcagc ggaggcgctt tatttaactc cacaaaccat taccggacag attcgagcgc tggaagagcg
0141 cctgcaaggc aaattattta aacgcaaggg acgtggtctc gaacccagcg agctgggaga actggtctat
0211 cgctatgccg ataaaatgtt caccttaagc caggaaatgc tggatattgt gaactatcgc aaagaatcca
0281 atttattgtt tgacgttggc gtggctgatg cactttccaa acgcctggtc agtagcgtac ttaacgccgc
0351 agtggtagaa ggcgagccca ttcatcttcg ctgcttcgaa tccacccacg aaatgctgct ggagcaatta
0421 agtcagcata aactggatat gatcatttct gactgtccga tagactctac gcagcaggaa ggcctgttct
0491 ccgtgagaat tggcgaatgt ggcgtgagtt tctggtgtac aaatccacca ccagaaaaac cgttcccggc
0561 ttgtctggaa gaacggcgac ttttgattcc tgggcgacgt tcaatgttag ggcgcaaatt gcttaactgg
0631 tttaactccc agggattaaa cgtagaaatc ctcggcgagt ttgatgatgc cgctttgatg aaagcttttg
0701 gtgcgatgca caatgcaatc ttcgttgccc caacgcttta tgcatatgac ttttatgccg ataaaactgt
0771 cgtagaaatt ggtcgcgtcg agaatgtgat ggaagagtac catgctattt ttgctgagcg gatgattcag
0841 cacccggcgg tacagcgaat ctgcaatacg gattattctg cgctttttag tccagcggtg cgttaaTCGG
0911 CAGCTCCCCC AAAGTTAAGG TGGGGGAGAT AGATTAGTTG TACATTACCA CGATTTTGAC TCGGCTCATT
0981 ATTTGCCCGC TTGAGACATT GTTTCC
|