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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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Deletion
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OCR30,31-3 (BK2-1 / AK1-1)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
murE
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Related strains
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Strains Search
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Search strains by gene name:
murE
Search strains by all related name:
murE b0085 ECK0086 JW0083
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General information (Go to Linear View: )
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Gene Name
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murE
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Alternative name
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b0085,ECK0086,JW0083
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Location, Length
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93,166 - 94,653
(
+
)
;
2.01
min
;
1488
(bp) ,
495
(aa)
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Product
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UDP-N-acetylmuramoyl-L-alanyl-D-glutamate:meso- diaminopimelate ligase
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Operon Name
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mraZ-rsmH-ftsLI-murEF-mraY-murD-ftsW-murGC-ddlB-ftsQAZ-lpxC
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Note
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GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_process: GO:0009252 - peptidoglycan biosynthetic process
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Function
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enzyme; Murein sacculus, peptidoglycan
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Gene Ontology
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GO:0000166
;
nucleotide binding ( murE )
GO:0005515
;
protein binding ( murE )
GO:0005524
;
ATP binding ( murE )
GO:0005737
;
cytoplasm ( murE )
GO:0007047
;
cell wall organization and biogenesis ( murE )
GO:0007049
;
cell cycle ( murE )
GO:0008360
;
regulation of cell shape ( murE )
GO:0008765
;
UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity ( murE )
GO:0009058
;
biosynthetic process ( murE )
GO:0009252
;
peptidoglycan biosynthetic process ( murE )
GO:0009273
;
peptidoglycan-based cell wall biogenesis ( murE )
GO:0016874
;
ligase activity ( murE )
GO:0016881
;
acid-amino acid ligase activity ( murE )
GO:0051301
;
cell division ( murE )
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PID
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1786273
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EC number
(KEGG Pathway)
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6.3.2.13
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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murE
(
EG10621
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
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MURE_ECOLI
(
P22188
;
O07101
)
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- Protein name
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UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase
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- Synonyms
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EC 6.3.2.13, UDP-N-acetylmuramyl-tripeptide synthetase, Meso- diaminopimelate-adding enzyme, UDP-MurNAc-tripeptide synthetase
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- Gene name
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Name=murE; OrderedLocusNames=b0085;
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MMBR References
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Biochemistry. 1997;36(20):6223-9.
Conditionally lethal Escherichia coli murein mutants contain point defects that map to regions conserved among murein and folyl poly-gamma-glutamate ligases: identification of a ligase superfamily.
Eveland SS, Pompliano DL, Anderson MS.
(
9166795
)
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J Bacteriol. 1989;171(11):6379-82.
Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes.
Jung HK, Ishino F, Matsuhashi M.
(
2553679
)
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J Bacteriol. 1989;171(11):6126-34.
Organization of the murE-murG region of Escherichia coli: identification of the murD gene encoding the D-glutamic-acid-adding enzyme.
Mengin-Lecreulx D, Parquet C, Desviat LR, Pla J, Flouret B, Ayala JA, van Heijenoort J.
(
2681153
)
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Biochem J. 1990;269(1):277-8.
Revised interpretation of the sequence containing the murE gene encoding the UDP-N-acetylmuramyl-tripeptide synthetase of Escherichia coli.
Michaud C, Parquet C, Flouret B, Blanot D, van Heijenoort J.
(
2198024
)
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Can J Microbiol. 1989;35(11):1051-4.
Nucleotide sequence of the murE gene of Escherichia coli.
Tao JS, Ishiguro EE.
(
2692800
)
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| Sequences |
Amino acid
FASTA format
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0001 MADRNLRDLL APWVPDAPSR ALREMTLDSR VAAAGDLFVA VVGHQADGRR YIPQAIAQGV AAIIAEAKDE
0071 ATDGEIREMH GVPVIYLSQL NERLSALAGR FYHEPSDNLR LVGVTGTNGK TTTTQLLAQW SQLLGEISAV
0141 MGTVGNGLLG KVIPTENTTG SAVDVQHELA GLVDQGATFC AMEVSSHGLV QHRVAALKFA ASVFTNLSRD
0211 HLDYHGDMEH YEAAKWLLYS EHHCGQAIIN ADDEVGRRWL AKLPDAVAVS MEDHINPNCH GRWLKATEVN
0281 YHDSGATIRF SSSWGDGEIE SHLMGAFNVS NLLLALATLL ALGYPLADLL KTAARLQPVC GRMEVFTAPG
0351 KPTVVVDYAH TPDALEKALQ AARLHCAGKL WCVFGCGGDR DKGKRPLMGA IAEEFADVAV VTDDNPRTEE
0421 PRAIINDILA GMLDAGHAKV MEGRAEAVTC AVMQAKENDV VLVAGKGHED YQIVGNQRLD YSDRVTVARL
0491 LGVIA
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GCCATCATGG GCGGCGTATT GCGTACCATG
-070 AACATCGAGC CGGATGCGCT GACAACGGGC GATAAAAATG AATTTGTGAT TAATCAAGGC GAGGGGACAG
0001 gtggcagatc gtaatttgcg cgaccttctt gctccgtggg tgccagacgc accttcgcga gcactgcgag
0071 agatgacact cgacagccgt gtggctgcgg cgggcgatct ctttgtagct gtagtaggtc atcaggcgga
0141 cgggcgtcga tatatcccgc aggcgatagc gcaaggtgtg gctgccatta ttgcagaggc gaaagatgag
0211 gcgaccgatg gtgaaatccg tgaaatgcac ggcgtaccgg tcatctatct cagccagctc aacgagcgtt
0281 tatctgcact ggcgggccgc ttttaccatg aaccctctga caatttacgt ctcgtgggcg taacgggcac
0351 caacggcaaa accacgacta cccagctgtt ggcgcagtgg agccaactgc ttggcgaaat cagcgcggta
0421 atgggcaccg ttggtaacgg cctgctgggg aaagtgatcc cgacagaaaa tacaaccggt tcggcagtcg
0491 atgttcagca tgagctggcg gggctggtgg atcagggcgc gacgttttgc gcaatggaag tttcctccca
0561 cgggctggta cagcaccgtg tggcggcatt gaaatttgcg gcgtcggtct ttaccaactt aagccgcgat
0631 caccttgatt atcatggtga tatggaacac tacgaagccg cgaaatggct gctttattct gagcatcatt
0701 gcggtcaggc gattattaac gccgacgatg aagtgggccg ccgctggctg gcaaaactgc cggacgcggt
0771 tgcggtatca atggaagatc atattaatcc gaactgtcac ggacgctggt tgaaagcgac cgaagtgaac
0841 tatcacgaca gcggtgcgac gattcgcttt agctcaagtt ggggcgatgg cgaaattgaa agccatctga
0911 tgggcgcttt taacgtcagc aacctgctgc tcgcgctggc gacactgttg gcactcggct atccactggc
0981 tgatctgctg aaaaccgccg cgcgtctgca accggtttgc ggacgtatgg aagtgttcac tgcgccaggc
1051 aaaccgacgg tggtggtgga ttacgcgcat acgccggatg cactggaaaa agccttacag gcggcgcgtc
1121 tgcactgtgc gggcaagctg tggtgtgtct ttggctgtgg tggcgatcgc gataaaggta agcgtccact
1191 gatgggcgca attgccgaag agtttgctga cgtggcggtg gtgacggacg ataacccgcg taccgaagaa
1261 ccgcgtgcca tcatcaacga tattctggcg ggaatgttag atgccggaca tgccaaagtg atggaaggcc
1331 gtgctgaagc ggtgacttgc gccgttatgc aggctaaaga gaatgatgtg gtactggtcg cgggcaaagg
1401 ccatgaagat taccagattg ttggcaatca gcgtctggac tactccgatc gcgtcacggt ggcgcgtctg
1471 ctgggggtga ttgcatgaTT AGCGTAACCC TTAGCCAACT TACCGACATT CTCAACGGTG AACTGCAAGG
1541 TGCAGATATC ACCCTTGATG CTGTAACCAC TGATACCCGA AAACTGAC
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