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PEC Original Annotations
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Essentiality
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Class
|
essential
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References (PMID)
|
J Bacteriol. 1997;179(20):6228-37.
Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.
Link AJ, Phillips D, Church GM.
(
9335267
)
|
|
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Deletion
|
OCR33,35-4 (AK4-2 / BK5-2)
(D)
,
OCR33-16
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
map
|
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Related strains
|
|
Strains Search
|
Search strains by gene name:
map
Search strains by all related name:
map b0168 ECK0166 JW0163 pepM
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General information (Go to Linear View: )
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Gene Name
|
map
|
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Alternative name
|
b0168,ECK0166,JW0163,pepM
|
|
Location, Length
|
188,712 - 189,506
(
-
)
;
4.07
min
;
795
(bp) ,
264
(aa)
|
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Product
|
methionine aminopeptidase
|
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Operon Name
|
map-glnD-dapD
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Note
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GO_process: GO:0006457 - protein folding
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Function
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enzyme; Proteins - translation and modification
|
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Gene Ontology
|
GO:0004177
;
aminopeptidase activity ( map )
GO:0004239
;
methionyl aminopeptidase activity ( map )
GO:0005515
;
protein binding ( map )
GO:0006508
;
proteolysis ( map )
GO:0008233
;
peptidase activity ( map )
GO:0008235
;
metalloexopeptidase activity ( map )
GO:0016787
;
hydrolase activity ( map )
GO:0046872
;
metal ion binding ( map )
GO:0050897
;
cobalt ion binding ( map )
|
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PID
|
1786364
|
EC number
(KEGG Pathway)
|
3.4.11.18
|
|
Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
|
EcoGene
|
map
(
EG10570
)
|
|
Number of removed
|
1 aa cleaved
|
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SWISS-PROT
(
Show details
)
|
Entry name(Acc.no)
|
AMPM_ECOLI
(
P07906
)
|
|
- Protein name
|
Methionine aminopeptidase
|
|
- Synonyms
|
EC 3.4.11.18, MAP, Peptidase M
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- Gene name
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OrderedLocusNames=b0168, c0203, z0178, ECs0170, SF0158, S0161;
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Homology Analysis
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BLAST
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Bacteria
|
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GTOP
|
map
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
Chain A, E.Coli Methionine Aminopeptidase His79ala Mutant
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Methionine aminopeptidase (MAP) (Peptidase M)
|
|
PSI-BLAST
|
Methionine aminopeptidase (MAP) (Peptidase M)
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nr
(database updated :
2007.02.20
)
|
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BLAST
|
methionine aminopeptidase [Escherichia coli O157:H7 EDL933]
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Pfam 28.0
(database updated :
2015-05
)
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Pfam
|
|
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PROSITE
|
|
PROSITE
|
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
MAP_1
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MMBR References
|
J Bacteriol. 1987;169(2):751-7.
Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure.
Ben-Bassat A, Bauer K, Chang SY, Myambo K, Boosman A, Chang S.
(
3027045
)
|
J Bacteriol. 1989;171(7):4071-2.
Methionine aminopeptidase gene of Escherichia coli is essential for cell growth.
Chang SY, McGary EC, Chang S.
(
2544569
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC
0071 ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV
0141 KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG
0211 KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 tggcgaaatt cagaatgatt ctcaatttgc
-070 ccgggtgtga taccattgac ggcacttaca tatatattgt cggtatcacc gacgctgatg gacagaatta
0001 atggctatct caatcaagac cccagaagat atcgaaaaaa tgcgcgtcgc tggccgactg gctgccgaag
0071 tgctggagat gatcgaaccg tatgttaaac cgggcgtcag caccggcgag ctggatcgca tctgtaatga
0141 ttacattgtt aatgaacaac acgcggtttc tgcctgcctc ggctatcacg gctatccgaa atccgtttgc
0211 atctctatta atgaagtggt gtgccacggt atcccggacg atgctaagct gctgaaagat ggcgatatcg
0281 ttaacattga tgtcaccgta atcaaagatg gtttccacgg cgatacctcg aaaatgttta tcgtcggtaa
0351 gccgaccatc atgggcgaac gtctgtgccg catcacgcaa gaaagcctgt acctggcgct acgcatggta
0421 aaaccaggca ttaatctgcg cgaaatcggt gcggcgattc agaaatttgt cgaagcagaa ggcttctccg
0491 tcgttcgtga atattgcgga cacggtattg gtcgcggctt ccatgaagaa ccgcaggtgc tgcactatga
0561 ctcccgtgaa accaacgtcg tactgaaacc tgggatgacg ttcaccatcg agccaatggt caacgcgggt
0631 aaaaaagaga tccgcaccat gaaagatggc tggacggtaa aaaccaaaga tcgcagcttg tctgcacaat
0701 atgagcatac tattgtggtg actgataacg gctgcgaaat tctgacgcta cgcaaggatg acaccatccc
0771 ggcgataatc tcgcacgacg aataagatga agccggcgaa tgccggcttt tttaatgcga taatttaatc
0841 ttatgggtgg cgcacaatga atacccttcc agaacagtac gcaaacaccg ctctc
|
