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PEC Original Annotations
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Essentiality
|
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Class
|
essential
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|
References (PMID)
|
Mol Gen Genet. 1983;192(1-2):10-4.
Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli.
Yamagata H, Taguchi N, Daishima K, Mizushima S.
(
6227793
)
|
|
|
Deletion
|
OCR27
(D)
,
OCR27-9
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
lspA
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
lspA
Search strains by all related name:
lspA b0027 ECK0028 JW0025
|
General information (Go to Linear View: )
|
|
Gene Name
|
lspA
|
|
Alternative name
|
b0027,ECK0028,JW0025
|
|
Location, Length
|
25,207 - 25,701
(
+
)
;
0.54
min
;
495
(bp) ,
164
(aa)
|
|
Product
|
prolipoprotein signal peptidase (signal peptidase II)
|
|
Operon Name
|
ribF-ileS-lspA-fkpB-ispH
|
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Note
|
prolipoprotein signal peptidase (SPase II); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane
|
|
Function
|
enzyme; Protein, peptide secretion
|
|
Gene Ontology
|
GO:0004190
;
aspartic-type endopeptidase activity ( lspA )
GO:0005886
;
plasma membrane ( lspA )
GO:0006508
;
proteolysis ( lspA )
GO:0008233
;
peptidase activity ( lspA )
GO:0009005
;
signal peptidase II activity ( lspA )
GO:0016020
;
membrane ( lspA )
GO:0016021
;
integral to membrane ( lspA )
GO:0016787
;
hydrolase activity ( lspA )
|
|
PID
|
1786210
|
EC number
(KEGG Pathway)
|
3.4.23.36
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
LSPA_ECOLI
(
P00804
)
|
|
- Protein name
|
Lipoprotein signal peptidase
|
|
- Synonyms
|
EC 3.4.23.36, Prolipoprotein signal peptidase, Signal peptidase II, SPase II
|
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- Gene name
|
Name=lspA; OrderedLocusNames=b0027;
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Homology Analysis
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BLAST
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Bacteria
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GTOP
|
lspA
(
homologous genes of other bacterias
)
|
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PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
Lipoprotein signal peptidase
|
|
PSI-BLAST
|
Lipoprotein signal peptidase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
signal peptidase II [Escherichia coli K12]
|
|
Pfam 28.0
(database updated :
2015-05
)
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Pfam
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PROSITE
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PROSITE
|
PKC_PHOSPHO_SITE
MYRISTYL
SPASE_II
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MMBR References
|
Proc Natl Acad Sci U S A. 1984;81:3708-3712
Nucleotide sequence of the Escherichia coli prolipoprotein signal peptidase (lsp) gene.
Innis, M. A., M. Tokunaga, M. E. Williams, J. M. Loranger, S.-Y. Chang, S. Chang, and H. C. Wu.
|
J Biol Chem. 1985;260:5616-5620
Characterization of the ileS-lsp operon in Escherichia coli. Identification of an open reading frame upstream of the ileS gene and potential promoter(s) for the ileS-lsp operon.
Kamio, Y., C.-K. Lin, M. Regue, and H. C. Wu.
|
J Biol Chem. 1987;262:389-393
Cotranscription of the isoleucyl-tRNA synthetase (ileS) and prolipoprotein signal peptidase(lsp) genes: fine-structure mapping of the lsp internal promoter.
Miller, K. W., and H. C. Wu.
|
J Bacteriol. 1984;158(2):632-5.
Mapping of the lipoprotein signal peptidase gene (lsp).
Regue M, Remenick J, Tokunaga M, Mackie GA, Wu HC.
(
6373724
)
|
J Biol Chem. 1985;260:5610-5615
Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.
Tokunaga, M., J. M. Loranger, S.-Y. Chang, M. Regue, S. Chang, and H. C. Wu.
|
Nucleic Acids Res. 1991;19(1):180
Nucleotide sequence of the lsp-dapB interval in Escherichia coli.
Bouvier J, Stragier P.
(
2011499
)
|
FEBS Lett. 1984;171(2):245-8.
The gene coding for lipoprotein signal peptidase (lspA) and that for isoleucyl-tRNA synthetase (ileS) constitute a cotranscriptional unit in Escherichia coli.
Yamada H, Kitagawa M, Kawakami M, Mizushima S.
(
6373377
)
|
FEBS Lett. 1983;158(2):301-4.
Cloning and expression of a gene coding for the prolipoprotein signal peptidase of Escherichia coli.
Yamagata H, Daishima K, Mizushima S.
(
6347715
)
|
Mol Gen Genet. 1983;192(1-2):10-4.
Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli.
Yamagata H, Taguchi N, Daishima K, Mizushima S.
(
6227793
)
|
FEBS Lett. 1984;173(1):264-8.
Nucleotide sequence of the lspA gene, the structural gene for lipoprotein signal peptidase of Escherichia coli.
Yu F, Yamada H, Daishima K, Mizushima S.
(
6378662
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MSQSICSTGL RWLWLVVVVL IIDLGSKYLI LQNFALGDTV PLFPSLNLHY ARNYGAAFSF LADSGGWQRW
0071 FFAGIAIGIS VILAVMMYRS KATQKLNNIA YALIIGGALG NLFDRLWHGF VVDMIDFYVG DWHFATFNLA
0141 DTAICVGAAL IVLEGFLPSR AKKQ
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 ACTACACCCA GGATGTCGGC AAGGTGGCGG
-070 AACACGCAGA AATCTGCGGC CGCTGTGTCA GCAACGTCGC CGGTGACGGT GAAAAACGTA AGTTTGCCTG
0001 atgagtcaat cgatctgttc aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc
0071 tgggcagcaa atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc cgtcgcttaa
0141 tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata gcggcggctg gcagcgttgg
0211 ttctttgccg gtattgcgat tggtattagc gtgatcctgg cagtgatgat gtatcgctcg aaggccacgc
0281 agaagctaaa caatatcgct tacgcgctga ttattggcgg cgcgctgggc aacctgttcg accgcctgtg
0351 gcacggcttc gttgtcgata tgatcgactt ctacgtcggc gactggcact tcgccacctt caaccttgcc
0421 gatactgcca tctgtgtcgg tgcggcactg attgtgctgg aaggtttttt gccttctaga gcgaaaaaac
0491 aataaTAAAC CCTGCCGGAT GCGATGCTGA CGCATCTTAT CCGGCCTACA GATTGCTGCG AAATCGTAGG
0561 CCGGATAAGG CGTTTACGCC GCATCCGGCA AAAAT
|
