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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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Mol Microbiol. 1992;6(7):817-23.
What is known about the structure and function of the Escherichia coli protein FirA?
Dicker IB, Seetharam S.
(
1602961
)
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Deletion
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OCR33,35-4 (AK4-2 / BK5-2)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lpxD
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Related strains
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Strains Search
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Search strains by gene name:
lpxD
Search strains by all related name:
lpxD b0179 ECK0178 fir firA hlpA JW0174 ompH omsA skp ssc
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General information (Go to Linear View: )
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Gene Name
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lpxD
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Alternative name
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b0179,ECK0178,fir,firA,hlpA,JW0174,ompH,omsA,skp,ssc
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Location, Length
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200,971 - 201,996
(
+
)
;
4.33
min
;
1026
(bp) ,
341
(aa)
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Product
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UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase
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Operon Name
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bamA-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE
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Note
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GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009245 - lipid A biosynthetic process
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Function
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enzyme; Surface polysaccharides & antigens
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Gene Ontology
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GO:0005515
;
protein binding ( skp )
GO:0008415
;
acyltransferase activity ( lpxD )
GO:0008610
;
lipid biosynthetic process ( lpxD )
GO:0009245
;
lipid A biosynthetic process ( lpxD )
GO:0016740
;
transferase activity ( lpxD )
GO:0016747
;
transferase activity, transferring groups other than amino-acyl groups ( lpxD )
GO:0042597
;
periplasmic space ( skp )
GO:0046677
;
response to antibiotic ( lpxD )
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PID
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1786376
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EC number
(KEGG Pathway)
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2.3.1.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
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lpxD
(
EG10316
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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LPXD_ECOLI
(
P21645
)
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- Protein name
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UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
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- Synonyms
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EC 2.3.1.-, FirA protein, Rifampicin resistance protein
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- Gene name
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Name=lpxD; Synonyms=firA, omsA; OrderedLocusNames=b0179;
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MMBR References
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J Bacteriol. 1988;170(12):5916-8.
Identity of the 17-kilodalton protein, a DNA-binding protein from Escherichia coli, and the firA gene product.
Aasland R, Coleman J, Holck AL, Smith CL, Raetz CR, Kleppe K.
(
3056922
)
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J Bacteriol. 1991;173(1):334-44.
Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli.
Dicker IB, Seetharam S.
(
1987124
)
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Eur J Biochem. 1993;212:363-369
Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli.
Helander, I. M., B. Lindner, U. Seydel, and M. Vaara.
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FEBS Lett. 1990;262(1):123-6.
Bacterial 'histone-like protein I' (HLP-I) is an outer membrane constituent?
Hirvas L, Coleman J, Koski P, Vaara M.
(
2318304
)
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J Bacteriol. 1991;173(3):1223-9.
The ompH gene of Yersinia enterocolitica: cloning, sequencing, expression, and comparison with known enterobacterial ompH sequences.
Hirvas L, Koski P, Vaara M.
(
1991717
)
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J Biol Chem. 1993;268:19866-19874
The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis.
Kelly, T. M., S. A. Stachula, C. R. H. Raetz, and M. S. Anderson.
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J Bacteriol. 1994;176:1639-1646
Mutations in firA, encoding the second acyltransferase in lipopolysaccharide biosynthesis, affect multiple steps in lipopolysaccharide biosynthesis.
Roy, A. M., and J. Coleman.
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Microbiol. Rev. 1993;57:655-682
Genetics of lipopolysaccharide biosynthesis in enteric bacteria.
Schnaitman, C. A., and J. D. Klena.
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J Bacteriol. 1988;170(3):1268-74.
First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.
Coleman J, Raetz CRH.
(
3277952
)
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| Sequences |
Amino acid
FASTA format
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0001 MPSIRLADLA QQLDAELHGD GDIVITGVAS MQSAQTGHIT FMVNPKYREH LGLCQASAVV MTQDDLPFAK
0071 SAALVVKNPY LTYARMAQIL DTTPQPAQNI APSAVIDATA KLGNNVSIGA NAVIESGVEL GDNVIIGAGC
0141 FVGKNSKIGA GSRLWANVTI YHEIQIGQNC LIQSGTVVGA DGFGYANDRG NWVKIPQIGR VIIGDRVEIG
0211 ACTTIDRGAL DDTIIGNGVI IDNQCQIAHN VVIGDNTAVA GGVIMAGSLK IGRYCMIGGA SVINGHMEIC
0281 DKVTVTGMGM VMRPITEPGV YSSGIPLQPN KVWRKTAALV MNIDDMSKRL KSLERKVNQQ D
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 CCAGGATATC GATCTGGTTG TTGATGCAAA
-070 CGCCGTTGCT TACAACAGCA GCGATGTAAA AGACATCACT GCCGACGTAC TGAAACAGGT TAAATAAGTA
0001 atgccttcaa ttcgactggc tgatttagcg cagcagttgg atgcagaact acacggtgat ggcgatatcg
0071 tcatcaccgg cgttgcgtcc atgcaatctg cacaaacagg tcacattacg ttcatggtta acccaaaata
0141 ccgtgagcat ttaggcttgt gccaggcgtc cgcggttgtc atgacccagg acgatcttcc tttcgcgaaa
0211 agtgccgcac tggtagtgaa gaatccctac ctgacttacg cgcgcatggc gcaaatttta gataccacgc
0281 cgcagcccgc gcagaacatt gcacccagtg cggtgatcga cgcgacggcg aagctgggta acaacgtatc
0351 gattggcgct aacgcggtga ttgagtccgg cgttgaactg ggcgataacg tgattatcgg tgccggttgc
0421 ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc tctgggcgaa cgtaaccatt taccatgaga
0491 tccagatcgg tcagaattgc ctgatccagt ccggaacagt ggtaggcgca gacggctttg gttatgccaa
0561 cgatcgtggt aactgggtga agatcccaca gattggtcgc gtaattattg gcgatcgcgt ggagatcggt
0631 gcctgcacaa ccatcgatcg cggcgcgctg gatgacacta ttattggcaa tggcgtgatc attgataacc
0701 agtgccagat tgcacataac gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca ttatggcggg
0771 cagcctgaaa attggtcgtt actgcatgat cggcggagcc agcgtaatca acgggcatat ggaaatatgc
0841 gacaaagtga cggttacggg catgggtatg gtgatgcgtc ccatcactga accaggcgtc tattcctcag
0911 gcattccgct gcaacccaac aaagtctggc gcaaaaccgc tgcactggtg atgaacattg atgacatgag
0981 caagcgtctg aaatcgcttg agcgcaaggt taatcaacaa gactaaCGTT CCATCTTTTG TTCGCCAAAC
1051 TTTACGGCCT GTCTCATTCT TACGATTGCG GCAGGCCGTG TTATTATTGT CGTTTCTTAT ATTTTGACAG
1121 GAAGAG
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