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PEC Original Annotations
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Essentiality
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Class
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essential
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References (PMID)
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J Biol Chem. 1994;269(52):32896-903.
An Escherichia coli gene (FabZ) encoding (3R)-hydroxymyristoyl acyl carrier protein dehydrase. Relation to fabA and suppression of mutations in lipid A biosynthesis.
Mohan S, Kelly TM, Eveland SS, Raetz CR, Anderson MS.
(
7806516
)
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Deletion
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OCR33,35-4 (AK4-2 / BK5-2)
(D)
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Related gene (W3110 PEC)
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Gene Search
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Search MG1655 PEC by gene name:
lpxA
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Related strains
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Strains Search
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Search strains by gene name:
lpxA
Search strains by all related name:
lpxA b0181 ECK0180 JW0176
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General information (Go to Linear View: )
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Gene Name
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lpxA
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Alternative name
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b0181,ECK0180,JW0176
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Location, Length
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202,560 - 203,348
(
+
)
;
4.37
min
;
789
(bp) ,
262
(aa)
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Product
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UDP-N-acetylglucosamine acetyltransferase
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Operon Name
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bamA-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE
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Note
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UDP-N-acetylglucosamine acetyltransferase; lipid A biosynthesis; GO_component: GO:0005737 - cytoplasm; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009245 - lipid A biosynthetic process
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Function
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enzyme; Surface polysaccharides & antigens
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Gene Ontology
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GO:0005737
;
cytoplasm ( lpxA )
GO:0008415
;
acyltransferase activity ( lpxA )
GO:0008610
;
lipid biosynthetic process ( lpxA )
GO:0008780
;
acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity ( lpxA )
GO:0009245
;
lipid A biosynthetic process ( lpxA )
GO:0016740
;
transferase activity ( lpxA )
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PID
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1786378
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EC number
(KEGG Pathway)
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2.3.1.129
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SWISS-PROT
(
Show details
[
1
more]
)
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Entry name(Acc.no)
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LPXA_ECOLI
(
P10440
;
P78243
)
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- Protein name
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Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
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- Synonyms
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EC 2.3.1.129, UDP-N-acetylglucosamine acyltransferase
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- Gene name
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Name=lpxA; OrderedLocusNames=b0181, c0218, SF0171, S0174;
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MMBR References
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J Biol Chem. 1990;265:6394-6402
A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis.
Galloway, A., and C. R. H. Raetz.
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J Bacteriol. 1991;173(11):3591-6.
Acyl-acyl carrier protein specificity of UDP-GlcNAc acyltransferases from gram-negative bacteria: relationship to lipid A structure.
Williamson JM, Anderson MS, Raetz CR.
(
1904441
)
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J Bacteriol. 1988;170(3):1268-74.
First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene.
Coleman J, Raetz CRH.
(
3277952
)
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J Bacteriol. 1986;168(1):152-9.
Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.
Crowell DN, Anderson MS, Raetz CRH.
(
3531165
)
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J Bacteriol. 1987;169(12):5727-34.
Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase.
Crowell DN, Reznikoff WS, Raetz CRH.
(
2824445
)
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| Sequences |
Amino acid
FASTA format
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0001 MIDKSAFVHP TAIVEEGASI GANAHIGPFC IVGPHVEIGE GTVLKSHVVV NGHTKIGRDN EIYQFASIGE
0071 VNQDLKYAGE PTRVEIGDRN RIRESVTIHR GTVQGGGLTK VGSDNLLMIN AHIAHDCTVG NRCILANNAT
0141 LAGHVSVDDF AIIGGMTAVH QFCIIGAHVM VGGCSGVAQD VPPYVIAQGN HATPFGVNIE GLKRRGFSRE
0211 AITAIRNAYK LIYRSGKTLD EVKPEIAELA ETYPEVKAFT DFFARSTRGL IR
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Nucleotide
FASTA format
View sequence out neighbor 100bp
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-100 GCGCCGCGGC CTGACCCGTT TTAAAGGGGT
-070 TGCTCTGGTC GATGGTAAAG TAGTTTGCGA AGCAACGATG ATGTGTGCTC GTAGCCGGGA GGCCTGATAC
0001 gtgattgata aatccgcctt tgtgcatcca accgccattg tggaagaggg cgcgtcaatt ggcgcgaacg
0071 cacacattgg tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac tgaaatctca
0141 cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc agttcgcctc catcggcgaa
0211 gttaaccagg atctgaaata tgctggcgaa ccgacccgtg tggaaatcgg cgatcgtaac cgcattcgcg
0281 aaagcgtcac cattcatcgt ggcacagtcc agggcggtgg attgacgaag gtgggcagcg acaacttact
0351 gatgatcaac gcgcacattg cgcacgattg tacggtaggt aaccgctgta ttctcgccaa caacgcaacg
0421 ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca
0491 tcattggtgc gcacgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt atgtcattgc
0561 gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc gccgcggatt cagccgtgag
0631 gcgattaccg ctatccgcaa tgcgtataag ctgatttatc gtagcggtaa aacgctcgat gaagtgaaac
0701 cggaaattgc tgaactggcg gaaacatatc cggaagtgaa agcctttacc gatttctttg cacgctcaac
0771 gcgcggtctg attcgttaaT GACTGAACAG CGTCCATTAA CGATTGCCCT GGTCGCCGGA GAAACCTCCG
0841 GCGATATCCT GGGGGCCGGT TTAATCCGCG CTCTGAAAGA ACATGTGCC
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