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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
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OCR45,46-3
(D)
,
OCR46
(D)
,
OCR46-7
(D)
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Related gene (W3110 PEC)
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Gene Search
|
Search MG1655 PEC by gene name:
lipA
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Related strains
|
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Strains Search
|
Search strains by gene name:
lipA
Search strains by all related name:
lipA b0628 ECK0621 JW0623 lip
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General information (Go to Linear View: )
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Gene Name
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lipA
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Alternative name
|
b0628,ECK0621,JW0623,lip
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Location, Length
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658,474 - 659,439
(
-
)
;
14.19
min
;
966
(bp) ,
321
(aa)
|
|
Product
|
lipoate synthase
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Operon Name
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lipA
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Note
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lipoate synthesis, sulfur insertion?; GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009107 - lipoate biosynthetic process
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Function
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putative enzyme; Biosynthesis of cofactors, carriers: Lipoate
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Gene Ontology
|
GO:0003824
;
catalytic activity ( lipA )
GO:0005506
;
iron ion binding ( lipA )
GO:0005737
;
cytoplasm ( lipA )
GO:0008152
;
metabolic process ( lipA )
GO:0009107
;
lipoate biosynthetic process ( lipA )
GO:0016740
;
transferase activity ( lipA )
GO:0016783
;
sulfurtransferase activity ( lipA )
GO:0016992
;
lipoate synthase activity ( lipA )
GO:0046872
;
metal ion binding ( lipA )
GO:0051536
;
iron-sulfur cluster binding ( lipA )
GO:0051539
;
4 iron, 4 sulfur cluster binding ( lipA )
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PID
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1786846
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EC number
(KEGG Pathway)
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2.8.1.-
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
|
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EcoGene
|
lipA
(
EG11306
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
LIPA_ECOLI
(
P60716
;
P25845; P77595
)
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- Protein name
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Lipoyl synthase
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- Synonyms
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EC 2.8.1.-, Lipoic acid synthase, Lipoate synthase, Lipoyl-acyl-carrier protein synthase, Sulfur insertion protein lipA, Lip-syn
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- Gene name
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Name=lipA; Synonyms=lip; OrderedLocusNames=b0628;
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MMBR References
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J Biol Chem. 1992;267:9512-9515
The biosynthesis of lipoic acid. Cloning of lip, a lipoate biosynthetic locus of Escherichia coli.
Hayden, M. A., I. Huang, D. E. Bussiere, and G. W. Ashley.
|
Biochemistry. 1993;32(14):3778-82.
Biosynthesis of lipoic acid: characterization of the lipoic acid auxotrophs Escherichia coli W1485-lip2 and JRG33-lip9.
Hayden MA, Huang IY, Iliopoulos G, Orozco M, Ashley GW.
(
8466915
)
|
J Bacteriol. 1993;175:1325-1336
Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes.
Reed, K. E., and J. E. Cronan, Jr.
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J Bacteriol. 1991;173(20):6411-20.
Lipoic acid metabolism in Escherichia coli: isolation of null mutants defective in lipoic acid biosynthesis, molecular cloning and characterization of the E. coli lip locus, and identification of the lipoylated protein of the glycine cleavage system.
Vanden Boom TJ, Reed KE, Cronan JE Jr.
(
1655709
)
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| Sequences |
Amino acid
FASTA format
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0001 MSKPIVMERG VKYRDADKMA LIPVKNVATE REALLRKPEW MKIKLPADST RIQGIKAAMR KNGLHSVCEE
0071 ASCPNLAECF NHGTATFMIL GAICTRRCPF CDVAHGRPVA PDANEPVKLA QTIADMALRY VVITSVDRDD
0141 LRDGGAQHFA DCITAIREKS PQIKIETLVP DFRGRMDRAL DILTATPPDV FNHNLENVPR IYRQVRPGAD
0211 YNWSLKLLER FKEAHPEIPT KSGLMVGLGE TNEEIIEVMR DLRRHGVTML TLGQYLQPSR HHLPVQRYVS
0281 PDEFDEMKAE ALAMGFTHAA CGPFVRSSYH ADLQAKGMEV K
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 gatatactgc cagtcgttaa ttcaaaaata
-070 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa cacgcacgct
0001 atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg
0071 ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
0141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa
0211 gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
0281 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
0351 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga ccgtgatgac
0421 ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca
0491 aaattgaaac tctggtgccg gatttccgcg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
0561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat
0631 tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
0701 tgatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
0771 gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg ttacgttagc
0841 ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt
0911 ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
0981 cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat cgaacaagat attgagggag
1051 cgtcctgctc gccacg
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