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PEC Original Annotations
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Essentiality
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Class
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non-essential
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References (PMID)
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Escherichia coli and Salmonella, Cellular and Molecular Biology Second Edition. ASM Press. 1996
Neidhart, F.C.
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Deletion
|
OCR30
(D)
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|
Related gene (W3110 PEC)
|
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Gene Search
|
Search MG1655 PEC by gene name:
leuA
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Related strains
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Strains Search
|
Search strains by gene name:
leuA
Search strains by all related name:
leuA b0074 ECK0076 JW0073
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General information (Go to Linear View: )
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Gene Name
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leuA
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Alternative name
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b0074,ECK0076,JW0073
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Location, Length
|
81,958 - 83,529
(
-
)
;
1.77
min
;
1572
(bp) ,
523
(aa)
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Product
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2-isopropylmalate synthase
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Operon Name
|
leuLABCD
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Note
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GO_component: GO:0005737 - cytoplasm; GO_process: GO:0009098 - leucine biosynthetic process
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Function
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enzyme; Amino acid biosynthesis: Leucine
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Gene Ontology
|
GO:0003824
;
catalytic activity ( leuA )
GO:0003852
;
2-isopropylmalate synthase activity ( leuA )
GO:0008152
;
metabolic process ( leuA )
GO:0008652
;
amino acid biosynthetic process ( leuA )
GO:0009082
;
branched chain family amino acid biosynthetic process ( leuA )
GO:0009098
;
leucine biosynthetic process ( leuA )
GO:0016740
;
transferase activity ( leuA )
GO:0019752
;
carboxylic acid metabolic process ( leuA )
GO:0046912
;
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer ( leuA )
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PID
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1786261
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EC number
(KEGG Pathway)
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2.3.3.13
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Verified Protein Starts
(data
compiled from literature and appropriate citations are available from EcoGene)
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EcoGene
|
leuA
(
EG11226
)
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Number of removed
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1 aa cleaved
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SWISS-PROT
(
Show details
[
2
more]
)
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Entry name(Acc.no)
|
LEU1_ECOLI
(
P09151
;
P78044
)
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- Protein name
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2-isopropylmalate synthase
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- Synonyms
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EC 2.3.3.13, Alpha-isopropylmalate synthase, Alpha-IPM synthetase
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- Gene name
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Name=leuA; OrderedLocusNames=b0074;
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MMBR References
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J Mol Biol. 1983;170(1):39-59.
Transcription initiation sites of the leucine operons of Salmonella typhimurium and Escherichia coli.
Gemmill RM, Jones JW, Haughn GW, Calvo JM.
(
6195343
)
|
J Bacteriol. 1991;173(5):1634-41.
Transcription attenuation-mediated control of leu operon expression: influence of the number of Leu control codons.
Bartkus JM, Tyler B, Calvo JM.
(
1999384
)
|
J Mol Biol. 1981;149(4):579-97.
Control of leu operon expression in Escherichia coli by a transcription attenuation mechanism.
Wessler SR, Calvo JM.
(
6171647
)
|
J Bacteriol. 1977;129(2):1078-90.
Isolation and characterization of lambda pleu bacteriophages.
Davis MG, Calvo JM.
(
320178
)
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| Sequences |
Amino acid
FASTA format
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0001 MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DVMEVGFPVS SPGDFESVQT IARQVKNSRV
0071 CALARCVEKD IDVAAESLKV AEAFRIHTFI ATSPMHIATK LRSTLDEVIE RAIYMVKRAR NYTDDVEFSC
0141 EDAGRTPIAD LARVVEAAIN AGATTINIPD TVGYTMPFEF AGIISGLYER VPNIDKAIIS VHTHDDLGLA
0211 VGNSLAAVHA GARQVEGAMN GIGERAGNCS LEEVIMAIKV RKDILNVHTA INHQEIWRTS QLVSQICNMP
0281 IPANKAIVGS GAFAHSSGIH QDGVLKNREN YEIMTPESIG LNQIQLNLTS RSGRAAVKHR MDEMGYKESE
0351 YNLDNLYDAF LKLADKKGQV FDYDLEALAF IGKQQEEPEH FRLDYFSVQS GSNDIATAAV KLACGEEVKA
0421 EAANGNGPVD AVYQAINRIT EYNVELVKYS LTAKGHGKDA LGQVDIVANY NGRRFHGVGL ATDIVESSAK
0491 AMVHVLNNIW RAAEVEKELQ RKAQHNENNK ETV
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Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 agcattaagc cagcacgcag tcaaacaaaa
-070 aacccgcgcc attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc
0001 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga
0071 gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt
0141 ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc atcgcccgcc aggttaaaaa cagccgcgta
0211 tgtgcgttag ctcgctgcgt ggaaaaagat atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct
0281 tccgtattca tacctttatt gccacttcgc caatgcacat cgccaccaag ctgcgcagca cgctggacga
0351 ggtgatcgaa cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc
0421 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca
0491 ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggcct
0561 gtatgaacgc gtgcctaaca tcgacaaagc cattatctcc gtacataccc acgacgattt gggcctggcg
0631 gtcggaaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg cgcaatgaac gggatcggcg
0701 agcgtgccgg aaactgttcc ctggaagaag tcatcatggc gatcaaagtt cgtaaggata ttctcaacgt
0771 ccacaccgcc attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg
0841 atcccggcaa acaaagccat tgttggcagc ggcgcattcg cacactcctc cggtatacac caggatggcg
0911 tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
0981 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa agaaagtgaa
1051 tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg
1121 atctggaggc gctggccttc atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag
1191 cgtgcagtct ggctctaacg atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca
1261 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact gaatataacg
1331 tcgaactggt gaaatacagc ctgaccgcca aaggccacgg taaagatgcg ctgggtcagg tggatatcgt
1401 cgctaactac aacggtcgcc gcttccacgg cgtcggcctg gctaccgata ttgtcgagtc atctgccaaa
1471 gccatggtgc acgttctgaa caatatctgg cgtgccgcag aagtcgaaaa agagttgcaa cgcaaagctc
1541 aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac
1611 ggtattggtc cggaagtgat gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tt
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