|
PEC Original Annotations
|
|
Essentiality
|
|
Class
|
essential
|
|
References (PMID)
|
J Gen Microbiol. 1984;130(6):1315-23.
Temperature-sensitive beta-lactam-tolerant mutants of Escherichia coli.
Shimmin LC, Vanderwel D, Harkness RE, Currie BR, Galloway CA, Ishiguro EE.
(
6384423
)
|
|
|
Deletion
|
OCR27
(D)
,
OCR27-9
(D)
|
|
Related gene (W3110 PEC)
|
|
Gene Search
|
Search MG1655 PEC by gene name:
ispH
|
|
Related strains
|
|
Strains Search
|
Search strains by gene name:
ispH
Search strains by all related name:
ispH b0029 ECK0030 JW0027 lytB yaaE
|
General information (Go to Linear View: )
|
|
Gene Name
|
ispH
|
|
Alternative name
|
b0029,ECK0030,JW0027,lytB,yaaE
|
|
Location, Length
|
26,277 - 27,227
(
+
)
;
0.57
min
;
951
(bp) ,
316
(aa)
|
|
Product
|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein
|
|
Operon Name
|
ribF-ileS-lspA-fkpB-ispH
|
|
Note
|
GO_component: GO:0005737 - cytoplasm; GO_process: GO:0042493 - response to drug
|
|
Function
|
|
|
Gene Ontology
|
GO:0005506
;
iron ion binding ( ispH )
GO:0005515
;
protein binding ( ispH )
GO:0008299
;
isoprenoid biosynthetic process ( ispH )
GO:0016114
;
terpenoid biosynthetic process ( ispH )
GO:0016491
;
oxidoreductase activity ( ispH )
GO:0019288
;
isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway ( ispH )
GO:0046872
;
metal ion binding ( ispH )
GO:0051536
;
iron-sulfur cluster binding ( ispH )
GO:0051538
;
3 iron, 4 sulfur cluster binding ( ispH )
GO:0051745
;
4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity ( ispH )
|
|
PID
|
1786212
|
EC number
(KEGG Pathway)
|
1.17.1.2
|
|
SWISS-PROT
(
Show details
[
2
more]
)
|
Entry name(Acc.no)
|
ISPH_ECOLI
(
P62623
;
P22565
)
|
|
- Protein name
|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
|
|
- Synonyms
|
EC 1.17.1.2
|
|
- Gene name
|
Name=ispH; Synonyms=lytB; OrderedLocusNames=b0029;
|
|
Homology Analysis
|
|
BLAST
|
|
Bacteria
|
|
GTOP
|
ispH
(
homologous genes of other bacterias
)
|
|
PDB
(database updated :
2007.02.20
)
|
|
PSI-BLAST
|
|
|
SWISS-PROT
(database updated :
2007.02.20
)
|
|
BLAST
|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
|
|
PSI-BLAST
|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
|
|
nr
(database updated :
2007.02.20
)
|
|
BLAST
|
4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli CFT073]
|
|
Pfam 28.0
(database updated :
2015-05
)
|
|
Pfam
|
|
|
PROSITE
|
|
PROSITE
|
ASN_GLYCOSYLATION
PKC_PHOSPHO_SITE
CK2_PHOSPHO_SITE
TYR_PHOSPHO_SITE
MYRISTYL
AMIDATION
|
|
MMBR References
|
J Bacteriol. 1993;175(4):1203-5.
Identification of the Escherichia coli lytB gene, which is involved in penicillin tolerance and control of the stringent response.
Gustafson CE, Kaul S, Ishiguro EE.
(
8432714
)
|
Can J Microbiol. 1992;38(9):975-8.
Genetic mapping of the lytA and lytB loci of Escherichia coli, which are involved in penicillin tolerance and control of the stringent response.
Harkness RE, Kusser W, Qi BJ, Ishiguro EE.
(
1464070
)
|
J Bacteriol. 1987;169(9):4396-8.
Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli.
Kusser W, Ishiguro EE.
(
3305487
)
|
J Bacteriol. 1998;180:1959-1961
Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species.
Potter, S., X. Yang, M. J. Boulanger, and E. E. Ishiguro.
|
Nucleic Acids Res. 1991;19(1):180
Nucleotide sequence of the lsp-dapB interval in Escherichia coli.
Bouvier J, Stragier P.
(
2011499
)
|
| Sequences |
Amino acid
FASTA format
|
0001 MQILLANPRG FCAGVDRAIS IVENALAIYG APIYVRHEVV HNRYVVDSLR ERGAIFIEQI SEVPDGAILI
0071 FSAHGVSQAV RNEAKSRDLT VFDATCPLVT KVHMEVARAS RRGEESILIG HAGHPEVEGT MGQYSNPEGG
0141 MYLVESPDDV WKLTVKNEEK LSFMTQTTLS VDDTSDVIDA LRKRFPKIVG PRKDDICYAT TNRQEAVRAL
0211 AEQAEVVLVV GSKNSSNSNR LAELAQRMGK RAFLIDDAKD IQEEWVKEVK CVGVTAGASA PDILVQNVVA
0281 RLQQLGGGEA IPLEGREENI VFEVPKELRV DIREVD
|
Nucleotide
FASTA format
View sequence out neighbor 100bp
|
-100 GGCGACTCCA TTACCGTTGA TTTCAACCAT
-070 CCGCTGGCCG GGCAGACCGT TCATTTTGAT ATTGAAGTGC TGGAAATCGA TCCGGCACTG GAGGCGTAAC
0001 atgcagatcc tgttggccaa cccgcgtggt ttttgtgccg gggtagaccg cgctatcagc attgttgaaa
0071 acgcgctggc catttacggc gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga
0141 tagcttgcgt gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc gatcctgatt
0211 ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagtcg cgatttgacg gtgtttgatg
0281 ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc ccgcgccagt cgccgtggcg aagaatctat
0351 tctcatcggt cacgccgggc acccggaagt ggaagggaca atgggccagt acagtaaccc ggaaggggga
0421 atgtatctgg tcgaatcgcc ggacgatgtg tggaaactga cggtcaaaaa cgaagagaag ctctccttta
0491 tgacccagac cacgctgtcg gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa
0561 aattgtcggt ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt acgcgccctg
0631 gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa ctccaaccgt ctggcggagc
0701 tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga tgcgaaagac atccaggaag agtgggtgaa
0771 agaggttaaa tgcgtcggcg tgactgcggg cgcatcggct ccggatattc tggtgcagaa tgtggtggca
0841 cgtttgcagc agctgggcgg tggtgaagcc attccgctgg aaggccgtga agaaaacatt gttttcgaag
0911 tgccgaaaga gctgcgtgtc gatattcgtg aagtcgatta aGTCATTAGC AGCCTAAGTT ATGCGAAAAT
0981 GCCGGTCTTG TTACCGGCAT TTTTTATGGA GAAAACATGC GTTTACCTAT CTTCCTCGAT ACTGACCCCG
1051 G
|
